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Ionic Relations and Polyol Metabolism of Marine Fungi in Relation to Their Environment

  • D. H. Jennings
Part of the NATO ASI Series book series (NSSA, volume 201)

Abstract

The filamentous Hyphomycete Dendryphiella salina is the most extensively studied marine fungus. It has been shown by radiotracer flux analysis and X-ray microanalysis that the cytoplasmic concentrations of K, Na and Cl in mycelium gowing in 500 mM NaCl is of the order of 51–88, 74–139 and 160 mM respectively. There appears to be no accumulation of salt in vacuoles. In vivo studies of the effect of K and NaCl on enzymes are in keeping with the above values. Polyols make a major contribution to the osmotic ballast. The total concentration at any one external water potential is relatively constant. However the proportions of the four individual polyols, glycerol, erythritol, arabitol and mannitol may differ according to the solute generating the water potential. Growth of the marine yeast Debaryomyces hansenii in continuous culture suggests that polyols as well as producing compatible osmotic ballast are also, through their metabolism, involved in energy dissipation via futile cycles under conditions when growth becomes limited by conditions other than carbon supply. Primary and secondary transport in marine fungi is proton-and not sodium-based but a large volume cell wall may be involved in maintaining the appropriate proton electrochemical potential gradient.

Keywords

Osmotic Potential Soluble Carbohydrate Marine Fungus Marine Yeast Debaryomyces Hansenii 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. [1]
    J. Kohlmeyer and E. Kohlmeyer, “Marine Mycology”, Academic Press, New York (1979)Google Scholar
  2. [2]
    D. H. Jennings, Some aspects of the physiology and biochemistry of marine fungi. Biol. Rev.58: 423 (1983)CrossRefGoogle Scholar
  3. [3]
    A. H. L. Chamberlain and S. T. Moss, The Thraustochytrids: a protist group with mixed affinities. Bio Systems. 21: 341 (1988)PubMedCrossRefGoogle Scholar
  4. [4]
    J. Kohlmeyer, Taxonomic studies of the marine Ascomycotina, in“The biology of marine fungi”, S. T. Moss, ed. Cambridge University Press, Cambridge (1986)Google Scholar
  5. [5]
    J. M. Wethered, E. C. Metcalf and D. H. Jennings, Carbohydrate metabolism in the fungus Dendryphiella satina. VIII. The contribution of polyols and ions to the mycelia] solute potential in relation to the external osmoticum. New Phytol. 101: 631 (1985)CrossRefGoogle Scholar
  6. [6]
    D. H. Jennings, Cations and filamentous fungi: invasion of the sea and hyphal functioning, in“Ion transport in plants”, W. P. Anderson, ed. Academic Press, London (1973)Google Scholar
  7. [7]
    J. C. B. MacDermott and D. H. Jennings, The relationship between the uptake of glucose and 3–0-methyl glucose and soluble carbohydrate and polysaccharide in the fungus Dendryphiella salina. J. Gen. Microbiol. 97: 193 (1976)CrossRefGoogle Scholar
  8. [8]
    D. H. Jennings, Polyol metabolism in fungi, Adv. Microbial Physiol., 25: 150 (1984)Google Scholar
  9. [9]
    N. J. W. Clipson and D. H. Jennings, The role of sodium and potassium in the generation of the osmotic potential of the marine fungus Dendryphiella satina. Mycol. Res. (in press) (1990)Google Scholar
  10. [10]
    N. J. W. Clipson, M. A. Hajibagheri and D. H. Jennings, Ion compartmentation in the marine fungus Dendryphiella satinain response to salinity: X-ray microanalysis. J. Exp. Bot.41: 199 (1990)CrossRefGoogle Scholar
  11. [11]
    N. J. W. Clipson, D. H. Jennings and J. L. Smith, The response to salinity at the microscopic level of the marine fungus Dendryphiella saliraNicot and Pugh as investigated stereologically. New Phytol. 113: 21 (1989)CrossRefGoogle Scholar
  12. [12]
    F. M. Paton and D. H. Jennings, Effect of sodium and potassium chloride and polyols on malate and glucose 6-phosphate dehydrogenases from the marine fungus Dendryphiella satina. Mycol. Res. 92: 470 (1988)CrossRefGoogle Scholar
  13. [13]
    J. M. Davies, C. Brownlee and D. H. Jennings, Electrophysiological evidence for an electrogenic proton pump and proton symport of glucose in the marine fungus Dendryphiella satina. J. Exp. Bot. 41: 449 (1990)CrossRefGoogle Scholar
  14. [14]
    A. Garrill and D. H. Jennings, unpublished data (1989)Google Scholar
  15. [15]
    M. F. J. Galpin and D. H. Jennings, A plasma-membrane ATPase from Dendryphiella satina: cation specificity and interaction with fusicoccin and cyclicAMP. Trans. Br., vtol. Soc.75: 37 (1980)CrossRefGoogle Scholar
  16. [16]
    E. B. G. Jones and D. H. Jennings, The effect of cations on the growth of fungi. New Phytol. 64: 85 (1965)Google Scholar
  17. [17]
    J. M. Davies, Ion transport in the marine fungus Dendryphiella satina, Ph.D. thesis, University of Liverpool (1989)Google Scholar
  18. [18]
    D. H. Jennings and J. S. Aynsley, Compartmentation and low temperature fluxes of potassium in mycelium of Dendryphiella satina. New Phytol. 70: 713 (1971)CrossRefGoogle Scholar
  19. [19]
    C. W. Slayman and C. L. Slayman, Potassium transport in Neurospora. Evidence for a multisite carrier at high pH. J. Gen. Physiol.55: 758 (1970)PubMedCrossRefGoogle Scholar
  20. [20]
    J. G. Commerford, P. T. N. Spencer-Phillips and D. H. Jennings, Membrane-bound ATPase activity, the properties of which are altered by growth in saline conditions isolated from the marine yeast Deharyompces hansenü. Trans. Br. Mycol. Soc. 85: 431 (1985)CrossRefGoogle Scholar
  21. [21]
    R. M. Burke and D. H. Jennings, The effect of sodium chloride on the growth characteristics of the marine yeast Debaryomyces hansenii in batch and continuous culture under carbon limitation and under potassium limitation. Mycol. Res. (in press)(1990)Google Scholar
  22. [22]
    D. W. Tempest and O. M. Neijssel, Growth yield values in relation to respiration, in“Diversity of bacterial respiratory systems, Vol. 1.” C. J. Knowles, ed. C.R.C. Press, Boca Raton, (1980)Google Scholar
  23. [23]
    D. W. Tempest and O. M. Neijssel, The status of YATPand maintenance energy as biologically interpretable phenomena. Ann. Rev. Microhiol.38: 459 (1984)CrossRefGoogle Scholar
  24. [24]
    E. A. Newsholme and B. Crabtree, Substrate cycles in metabolic regulation and in heat generation. Biochem. Soc. Symp.41: 61 (1976)PubMedGoogle Scholar
  25. [25]
    D. H. Jennings and R. M. Burke, Compatible solutes - the mycological dimension and their role as physiological buffering agents. New Phytol. submitted. (1990)Google Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • D. H. Jennings
    • 1
  1. 1.Department of Genetics & MicrobiologyThe UniversityLiverpoolUK

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