Abstract
Androgenic hormones regulate the development and growth of many normal and tumor cells. It is clear that the androgen receptor (AR) plays a key role in the mechanism of androgen action. Activation of the androgen receptor by binding of the male hormone (either testosterone or dihydrotestosterone) results in the differential regulation of both mRNA and protein synthesis in target cells (1-5). Cloning of the androgen receptor cDNA, (the last of the known steroid receptor genes) has been achieved in several laboratories, including our own (6-10). Sequence comparison with the other steroid hormone receptor genes has revealed that the androgen receptor gene contains two highly conserved regions. Functional analysis of the estrogen- (11), progesterone- (12), and glucocorticoid receptor cDNA’s (13) has demonstrated that these two discrete regions correspond to DNA-binding and steroid-binding, respectively.
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References
Compere S.J., McKnight G.S., Palmiter R.D. Androgens regulate ovomucoid and ovalbumin gene expression independently of estrogen. J. Biol. Chem. 256:6341–6347, 1981.
Derman E. Isolation of a cDNA clone for mouse urinary proteins: age-and sex-related expression of mouse urinary protein genes is transcriptionally controlled. Proc. Natl.Acad. Sci. USA 78:5425–5429, 1981.
Berger F.G., Loose D., Meisner H., Watson G. Androgen induction of messenger RNA concentrations in mouse kidney is posttranscriptional. Biochemistry 25:1170–1175, 1986.
Catterall J.F., Janne O.A. Androgen action in mouse kidney: Structure and regulation of the β-glucuronidase and ornithine decarboxylase genes. Modern Cell Biol. 6:1–28, 1988.
Allison J., Zhang Y.-L., Parker M.G. Tissue-specific and hormonal regulation of the gene for rat prostatic steroid binding protein in transgenic mice. Mol. Cell Biol. 9:2254–2257, 1989.
Chang C., Kokontis J., Liao S. Structural analysis of complementary DNA and amino acid sequences of human and rat androgen receptors. Proc. Natl. Acad. Sci. USA 85:7211–7215, 1988.
Lubahn D.E., Joseph D.R., Sar M., Tan J., Higgs H.N., Larson R.E., French F.S., Wilson E.M. The human androgen receptors: Complementary deoxyribonucleic acid cloning, sequence analysis and gene expression in prostate. Mol. Endocrinol. 2:120651275, 1988.
Tilley W.D., Marcelli M., Wilson J.D., McPhaul M.J. Characterization and expression of a cDNA encoding the human androgen receptor. Proc. Natl. Acad. Sci. USA 86:327–331, 1989.
Trapman J., Klaaseen P., Kuiper G.G.J.M., van der Korput J.A.G.M., Faber P.W., van Rooij H.C.J., Geurts van Kessel A., Voerhorst M.M., Mulder E., Brinkmann A.O. Cloning, structure and expression of a cDNA encoding the human androgen receptor. Biochem. Biophys. Res. Com. 153:241–248, 1988.
Young C.Y.F., Johnson M.P., Prescott J., Heydrick K., Murthy L., Tindall D.J. Isolation of putative androgen receptor cDNA clones from a lambda gtll library constructed from the androgen receptor-rich cell line, LNCaP, using a steroid receptor DNA-binding consensus probe. Program of the 70th Annual Meeting of the Endocrine Society, New Orleans, LA, 1988, p. 93 (abstract), 1988.
Kumar V., Green S., Staub A., Chambon P. Localization of the oestradiol-binding and putative DNA-binding domains of the human oestrogen receptor. EMBO J 5:2231–2236, 1986.
Gronemeyer H., Tureotte B., Quirin-Stricker C., Bocquel M.T., Meyer M.E., Krozowski Z., Jeltsch J.M., Lerouge T., Garnier J.M., Chambon P. The chicken progesterone receptor sequence, expression and functional analysis. EMBO J 6:3985–3994, 1987.
Rusconi S., Yamamoto K.R. Functional dissection of the hormone and DNA binding activities of the glucocorticoid receptor. EMBO J 6:1309–1315, 1987.
Harris T.J. Expression of eukaryotic genes in E. coli. Genetic Engineering 4:127–185, 1983.
Belter M., Chang C.P., Robinson R.R., Horwitz A.H. Escherichia coli secretion of an active chimeric antibody fragment. Science 240:1041–1043, 1988.
Skerra A., Pluckthum A. Assembly of a functional immunoglobin Fv fragment in Escherichia coli. Science 260:1038–1104, 1988.
Freedman L.P., Luisi B.F., Korszum Z.R., Basavappa R., Sigler P.B., Yamamoto K.R. The function and structure of the metal coordination sites within the glucocorticoid receptor DNA binding region. Nature 334:563–546, 1988.
Eul J., Meyer M.E., Tora L., Bocquel M.T., Quirin-Stricker C., Chambon P., Gronemeyer H. Expression of active hormone and DNA-binding domains of the chicken progesterone receptor in E. coli. EMBO J 8:83–90, 1989.
Young C.Y.F., Murthy L.R., Prescott J.L., Johnson M.P., Rowley D.R., Cunningham G.R., Killian C.S., Scardino P.T., von Eschenbach A., Tindall D.J. Monoclonal antibodies against the androgenreceptor: Recognition of human and other mammalian androgen receptors. Endocrinology 123:601–610, 1988.
Scholtissek S., Grosse F. A plasmid vector system for the expression of a triprotein consisting of βgalactosidase, a collagenase recognition site and a foreign gene product. Gene 62:55–64, 1988.
Chang C.H., Rowley D.R., Tindall D.J. Purification and characterization of the androgen receptor from rat ventral prostate. Biochemistry 22:6170–6175, 1983.
Chang C.H., Rowley D.R., Lobi T.J., Tindall D.J. Purification and characterization of androgen receptors from steer seminal vesicle. Biochemistry 21:4102–4109, 1982.
Leff S.E., Rosenfeld M.G., Evans R.M. Complex transcriptional units: Diversity in gene expression by alternative RNA processing. Ann. Rev. Biochem. 55:1091–1117, 1986.
Hollenberg S.M., Weinberger C., Ong E.S., Cerelli G., Oro A., Lebo R., Thompson E.B., Rosenfeld M.G., Evans R.M. Primary structure and expression of a functional human, glucocorticoid receptor cDNA. Nature 318:635–641, 1985.
Walter P., Greene S., Green G., Kurst A., Bornert J.-M., Jeltsch J.M., Staub A., Jensen E., Scrace G., Waterfield M., Chambon P. Cloning of the human estrogen receptor cDNA. Proc. Natl. Acad. Sci. USA 82:7889–7893, 1985.
Arrija J.L., Weinberger C., Cerelli G., Glaser T.M., Handelin B.L., Housman D.E., Evans R.M. Cloning of human minerelocorticoid receptor complementary DNA: Structural and functional kinship with the glucocorticoid receptor. Science 237:268–275, 1987.
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Young, C.YF., Qiu, SD., Prescott, J.L., Tindall, D.J. (1991). Molecular Biology of Androgen Receptors in Prostate Cancer Cells. In: Karr, J.P., Coffey, D.S., Smith, R.G., Tindall, D.J. (eds) Molecular and Cellular Biology of Prostate Cancer. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3704-5_39
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DOI: https://doi.org/10.1007/978-1-4615-3704-5_39
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