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cDNA Cloning, Antibody Production and Immunohistochemical Localization of the Androgen Receptor

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Molecular and Cellular Biology of Prostate Cancer

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Summary

We describe the isolation and cloning of cDNAs encoding human and rat androgen receptors (ARs) and characterization of AR proteins expressed from the cDNAs. In addition we describe the production of trpE-AR fusion proteins and their use as antigens in the production of polyclonal and monoclonal anti-AR antibodies. Immunohistochemical staining of mouse, rat and human tissues revealed widespread tissue distribution of AR and subcellular localization of AR in the cell nucleus.

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Author information

Authors and Affiliations

  1. Department of Surgery/Urology, Chicago, Illinois, USA

    Chawnshang Chang, Hiroyuki Takeda & John Kokontis

  2. The Ben May Institute, The University of Chicago, Chicago, Illinois, USA

    Chawnshang Chang & John Kokontis

Authors
  1. Chawnshang Chang
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  2. Hiroyuki Takeda
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  3. John Kokontis
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Editor information

Editors and Affiliations

  1. Office of Scientific Administration, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY, 14263, USA

    James P. Karr Ph.D. (Chief) (Chief)

  2. The Johns Hopkins Hospital and University, Marburg Building, 600 North Wolfe Street, Baltimore, MD, 21205, USA

    Donald S. Coffey Ph.D. (Professor of Urology, Oncology and Pharmacology) (Professor of Urology, Oncology and Pharmacology)

  3. Department of Growth Biochemistry and Physiology, Merck, Sharpe and Dohme Research Lab, 126 East Lincoln Avenue, Rahway, NJ, 07065, USA

    Roy G. Smith Ph.D.

  4. Mayo Clinic, Guggenheim-4 200 First Street, SW, Rochester, MN, 55905, USA

    Donald J. Tindall Ph.D. (Professor of Urologic Research) (Professor of Urologic Research)

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© 1991 Springer Science+Business Media New York

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Chang, C., Takeda, H., Kokontis, J. (1991). cDNA Cloning, Antibody Production and Immunohistochemical Localization of the Androgen Receptor. In: Karr, J.P., Coffey, D.S., Smith, R.G., Tindall, D.J. (eds) Molecular and Cellular Biology of Prostate Cancer. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3704-5_21

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  • DOI: https://doi.org/10.1007/978-1-4615-3704-5_21

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6647-8

  • Online ISBN: 978-1-4615-3704-5

  • eBook Packages: Springer Book Archive

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