Protein C: Gene Structure and Protein Synthesis
Protein C is a vitamin K-dependent plasma glycoprotein that, after activation by thrombin-thrombomodulin upon the endothelial cell surface, serves as a feedback down-regulator of the coagulation cascade by specifically degrading the protein cofactors VIIIa and Va (Figure 1). The biological role of protein C and the vascular endothelium has been recently reviewed in a succinct fashion1. Protein C in a less understood manner also enhances the process of fibrinolysis. Because of its antithrombotic as well as pro-fibrinolytic properties, protein C is considered to be a potential agent for antithrombotic therapy and a candidate for production by recombinant DNA technologies. The scope of this paper is to review: (i) the organization of the human protein C gene, particularly as it relates to the genes for homologous vitamin K-dependent proteins and to genetic protein C deficiency; and (ii) the biosynthesis of recombinant protein C in forms that are properly processed and biologically active.
KeywordsHuman Protein Baby Hamster Kidney Amidolytic Activity Serine Protease Domain Genetic Protein
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