Factor IX: Gene Structure and Protein Synthesis

  • D. B. C. Ritchie
  • D. L. Robertson
  • R. T. A. MacGillivray


The development of recombinant DNA techniques during the past ten years has led to an explosion of the field of molecular genetics. Using these techniques, DNA fragments can be cloned and characterized at the molecular level. This in turn led to the discovery of intervening sequences in some eukaryotic genes, the identification of promoter elements, and to the production of recombinant proteins. As with many other fields, the field of thrombosis and hemostasis has also been changed by the application of recombinant DNA techniques. In this manuscript, we will review the structure and expression of the human factor IX gene, and discuss the various approaches to producing recombinant factor IX as a pharmaceutical. The molecular genetics of factor IX deficiency (hemophilia B) are discussed elsewhere in this book. The molecular biology and molecular genetics of blood coagulation have been reviewed recently1, 2.


Transcription Start Site Human Factor Long Terminal Repeat Chloramphenicol Acetyl Transferase Major Late Promoter 
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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • D. B. C. Ritchie
    • 1
  • D. L. Robertson
    • 1
  • R. T. A. MacGillivray
    • 1
  1. 1.Department of BiochemistryUniversity of British ColumbiaVancouverCanada

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