Estrogen Dependent Growth of a Rat Pituitary Tumor (MtT/F84)

  • A. Ito
  • N. Fujimoto
  • B. Roy
  • K. Inoue


An estrogen dependent transplantable pituitary tumor, designated MtT/F84, was induced in an estrogenized female F344 rat and has been serially passaged in 17 β-estradiol treated female rats. It grew well in rats treated with estrone, 17 β-estradiol (E2) or estriol but not in ovariectomized females or in rats given progesterone or testosterone. Growth of MtT cells about 105 when mixed with 1.1 or 11 μg grafted site of E2 in vitro and grafted into s.c., were much enhanced of their growth compared with the same number of MtT cells similarly grafted without E2 on the other side of breast chain in same ovariectomized rat. This local growth promoting effect of E2 on MtT was inhibited by the subsequent injection of tamoxifen at grafted site. Nuclear estrogen receptor (ER) levels correlatively increased according to the administered doses of E2, but cytosolic ER reversely decreased with the increase of E2 administration.


Estrogen Receptor Pituitary Tumor Clonal Cell Line Estrogen Receptor Level Nuclear Estrogen Receptor 
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  1. 1.
    F. Vignon, F. Capony, M. Chambon, G. Freias, M. Garcia, and H. Rochefort, Autocrine growth stimulation of MCF-7 breast cancer cells by the estrogen regulated 52 K protein, Endocrinology 118: 1537 (1986).PubMedCrossRefGoogle Scholar
  2. 2.
    L. J. Murphy, L. C. Murphy, and H. G. Friesen, Estrogen induction of N-myc and C-myc proto-oncogene expression in the rat uterus, Endocrinology 120: 1882 (1987).PubMedCrossRefGoogle Scholar
  3. 3.
    H. D. Soule, and C. M. McGrath, Erstrogen responsive proliferation of clonal human breast carcinoma in athymic mice, Cancer Lett. 10: 177 (1980).PubMedCrossRefGoogle Scholar
  4. 4.
    A. Manni, P. Wright, L. Feil, L. Baranao, L. Demers, M. Garcia, and H. Rochefort, Autocrine stimulation by estradiol regulated growth factors of rat hormone responsive mammary cancer: induction with polyamine pathway, Cancer Res. 46: 1594 (1986).PubMedGoogle Scholar
  5. 5.
    A. Ito, M. Naito, H. Watanabe, K. Yokoro, Prolactin and aging: X-irradiated and estrogen-induced rat mammary tumorigenesis, J. Natl. Cancer Inst. 107: 405 (1984).Google Scholar
  6. 6.
    A. Ito, K. Kawashima, N. Fujimoto, H. Watanabe, and M. Naito, Inhibition by 2-bromo-α-ergocriptine and tamoxifen of the growth of an estrogen-dependent transplantable pituitary tumor (MtT/F84) in F344 rats, Cancer Res. 45: 6436 (1985).PubMedGoogle Scholar
  7. 7.
    M. N. Gould, W. F. Biel, and K. H. Clifton, Morphological and quantitative studies of gland formation from inocula of monodispersed rat mammary cells, Exp. Cell Res. 107: 405 (1977).PubMedCrossRefGoogle Scholar
  8. 8.
    M. Ginsburg, B. D. Greenstein, N. J. MacLusky, I. D. Morris, and P. J. Thomas, An improved method for the study of high-affinity steroid binding: oestradiol binding in brain and pituitary, Steroids 23: 773 (1974).PubMedGoogle Scholar
  9. 9.
    K. Inoue, A. Hattori, T. Sasaki, S. Inukai, N. Fujimoyo, and A. Ito, Establishment of a series of pituitary clonal cell lines differing in morphology, hormone secretion, and response to estrogen, Endocrinology 126: 2313–2320, (1990).PubMedCrossRefGoogle Scholar
  10. 10.
    T. Kakunaga, and H. Yamasaki, Tranformation assay of established cell lines: Mechanisms and application, World Health Organization 67: 207 (1985).Google Scholar
  11. 11.
    K. H. Clifton, and R. Meyer, Mechanism of anterior pituitary tumor induction by estrogen, Anat. Res. 125: 65 (1956).Google Scholar
  12. 12.
    M. Mori, M. Naito, and A. Ito, Effects of sex hormone on MNU-induced thyroid tumors in rats, Proc. Jap. Cancer Ass. 47: 126 (1988).Google Scholar
  13. 13.
    A. H. Tashijan, Y. Yasumura, L. Levine, G. H. Sato, and M. L. Parker, Establishment of clonal strains of rat pituitary tumor cells that secrete growth hormone, Endocrinology 82: 342 (1968).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • A. Ito
    • 2
  • N. Fujimoto
    • 2
  • B. Roy
    • 2
  • K. Inoue
    • 1
  1. 1.Department of Cancer Research, Research Inst. for Nuclear Medicine & BiologyHiroshima Univ.HiroshimaJapan
  2. 2.Department of MorphologyInst. of Endocrinology Gunma Univ.MaebashiJapan

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