Restriction Polymorphisms in Tyrosine Kinase Genes in Cells Transformed by Reactive Oxidants: Evidence for Concerted Methylation Changes in a Family of Genes

  • C. J. Schmeichel
  • S. T. Satek
  • S. A. Weitzman
Part of the Developments in Oncology book series (DION, volume 71)


The restriction map of the tyrosine kinase gene c-abl is altered at specific CCGG sites in DNA from oxidant-transformed 10T1/2 mouse fibroblasts (1,2). Similar polymorphisms, which appear to be related to an unusual type of cytosine methylation, have now been detected in other non-receptor tyrosine kinase genes c-yes, c-src, and c-fyn. Such polymorphisms were not seen in other tyrosine kinase genes fc-fes, c-lyn. c-hck. erbB2), nor in other non-kinase oncogenes. These studies suggest that reactive oxidants may produce a concerted, covalent modification in susceptible regions of function- and sequence-related genes which are important in normal tissue development.


Cytosine Methylation CCGG Site Restriction Polymorphism Tyrosine Kinase Gene Ured Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Weitzman, S.A., Lee, R.M. and Ouellette, A.J. Biochem. Biophys. Res. Comm. 158:24–30, 1989.PubMedCrossRefGoogle Scholar
  2. 2.
    Schmeichel, C.J. and Weitzman, S.A. Free Radical Res. Comm. 12:761–769, 1991.CrossRefGoogle Scholar
  3. 3.
    Cerutti, P.A., Trump, B.F. Cancer Cells 3:1–7, 1991.PubMedGoogle Scholar
  4. 4.
    Goldstein, B.D., Witz, G. Free Radical Res. Comm. 11:3–10, 1990.CrossRefGoogle Scholar
  5. 5.
    Weitzman, S.A., Gordon, L.I. Blood 76:655–663, 1990.PubMedGoogle Scholar
  6. 6.
    Kensler, T.W., Egner, P.A., Taffe, B.G., Trush, M.A. Prog. Clin. Biol. Res 298:233–48, 1989.PubMedGoogle Scholar
  7. 7.
    Halliwell, B., Aruoma, O.I. FEBS Lett. 281:9–19, 1991.PubMedCrossRefGoogle Scholar
  8. 8.
    Weitzman, S.A., Weitberg, A.B., Clark, E.P., Stossel. T.P. Science 227:1231–1233, 1985.PubMedCrossRefGoogle Scholar
  9. 9.
    Ausubel, F.M., et al, eds.: Current Protocols in Molecular Biology. New York, Wiley lntrscience, 1987.Google Scholar
  10. 10.
    Cedar, H., Razin, A. Biochem. Biophys. Acta 1049:1–8, 1990.PubMedCrossRefGoogle Scholar
  11. 11.
    Smith, S.S., Tolberg, M.E. in: Biochemistry and Biology of DNA methylation (G.L. Cantoni and A. Razin, eds.) New York, Alan R. Liss Inc., 1985, pp 11–22.Google Scholar
  12. 12.
    Evans, M.J., Scarpulla, R.C. Mol. Cell. Biol. 8:35–41, 1988.PubMedGoogle Scholar
  13. 13.
    Prendergast, G.C., Ziff, E.B. Science 251:186–189, 1991.PubMedCrossRefGoogle Scholar
  14. 14.
    Cantley, L.C., Auger, K.R., Carpenter, C., et al. Cell 64:281–302, 1991.PubMedCrossRefGoogle Scholar
  15. 15.
    Hunter, T. Cell 64:249–270, 1991.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • C. J. Schmeichel
    • 1
  • S. T. Satek
    • 1
  • S. A. Weitzman
    • 1
  1. 1.Division of Hematology/Oncology, Department of Medicine and Cancer CenterNorthwestern University Medical SchoolChicagoUSA

Personalised recommendations