Restriction Polymorphisms in Tyrosine Kinase Genes in Cells Transformed by Reactive Oxidants: Evidence for Concerted Methylation Changes in a Family of Genes
The restriction map of the tyrosine kinase gene c-abl is altered at specific CCGG sites in DNA from oxidant-transformed 10T1/2 mouse fibroblasts (1,2). Similar polymorphisms, which appear to be related to an unusual type of cytosine methylation, have now been detected in other non-receptor tyrosine kinase genes c-yes, c-src, and c-fyn. Such polymorphisms were not seen in other tyrosine kinase genes fc-fes, c-lyn. c-hck. erbB2), nor in other non-kinase oncogenes. These studies suggest that reactive oxidants may produce a concerted, covalent modification in susceptible regions of function- and sequence-related genes which are important in normal tissue development.
KeywordsCytosine Methylation CCGG Site Restriction Polymorphism Tyrosine Kinase Gene Ured Cell
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