Long term Incubation of Cardiac Myocytes with Oleic Acid and Very-low Density Lipoprotein Reduces Heparin-releasable Lipoprotein Lipase Activity

  • Brian Rodrigues
  • Michael R. Spooner
  • David L. Severson
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 8)


An exogenous [3H]triolein emulsion was hydrolyzed by intact cardiac myocytes with functional LPL located on the cell surface. This surface-bound LPL could be released into the medium when cardiac myocytes were incubated with heparin. Incubation of cardiac myocytes with VLDL, or the products of TG breakdown, oleic acid or 2-monoolein, did not increase LPL activity in the medium. However, incubation of cardiac myocytes with either VLDL or oleic acid for > 60 min did reduce heparin-releasable LPL activity. In the heart, this inhibitory effect of FF A could regulate the translocation of LPL from its site of synthesis in the cardiac myocyte to its functional site at the capillary endothelium. (Mol Cell Biochem 116: 33–37, 1992).


lipoprotein lipase cardiac myocytes free fatty acids 


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Copyright information

© Springer Science+Business Media Dordrecht 1992

Authors and Affiliations

  • Brian Rodrigues
    • 1
  • Michael R. Spooner
    • 1
  • David L. Severson
    • 1
  1. 1.Department of Pharmacology & Therapeutics Faculty of MedicineThe University of CalgaryCalgaryCanada

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