Long term Incubation of Cardiac Myocytes with Oleic Acid and Very-low Density Lipoprotein Reduces Heparin-releasable Lipoprotein Lipase Activity

  • Brian Rodrigues
  • Michael R. Spooner
  • David L. Severson
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 8)

Abstract

An exogenous [3H]triolein emulsion was hydrolyzed by intact cardiac myocytes with functional LPL located on the cell surface. This surface-bound LPL could be released into the medium when cardiac myocytes were incubated with heparin. Incubation of cardiac myocytes with VLDL, or the products of TG breakdown, oleic acid or 2-monoolein, did not increase LPL activity in the medium. However, incubation of cardiac myocytes with either VLDL or oleic acid for > 60 min did reduce heparin-releasable LPL activity. In the heart, this inhibitory effect of FF A could regulate the translocation of LPL from its site of synthesis in the cardiac myocyte to its functional site at the capillary endothelium. (Mol Cell Biochem 116: 33–37, 1992).

Keywords

lipoprotein lipase cardiac myocytes free fatty acids 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Eckel RH: Lipoprotein lipase. A multifunctional enzyme relevant to common metabolic diseases. New Engl J Med 320: 1060–1068, 1989Google Scholar
  2. 2.
    Camp L, Reina M, Llobera M, Vilaro S, Olivecrona T: Lipoprotein lipase: cellular origin and functional distribution. Am J Physiol 258: C673–C681, 1990Google Scholar
  3. 3.
    Blanchette-Mackie EJ, Masuno H, Dwyer NK, Olivecrona T, Scow RO: Lipoprotein lipase in myocytes and capillary endothelium of heart: immunocytochemical study. Am J Physiol 256: E818–E828, 1989PubMedGoogle Scholar
  4. 4.
    Ben-Zeev O, Schwalb H, Schotz MC: Heparin-releasable and nonreleasable lipoprotein lipase in the perfused rat heart. J Biol Chem 256: 10550–10554, 1981PubMedGoogle Scholar
  5. 5.
    Severson DL, Lee M, Carroll R: Secretion of lipoprotein lipase from myocardial cells isolated from adult rat hearts. Mol Cell Biochem 79: 17–24, 1988PubMedCrossRefGoogle Scholar
  6. 6.
    Braun JEA, Severson DL: Diabetes reduces heparin-and phospholipase C-releasable lipoprotein lipase from cardiomyocytes. Am J Physiol 260: E477–E485, 1991PubMedGoogle Scholar
  7. 7.
    Severson DL, Carroll R: Effect of taxol on the heparin-induced secretion of lipoprotein lipase from cardiac myocytes. Mol Cell Biochem 88: 17–22, 1989PubMedCrossRefGoogle Scholar
  8. 8.
    Saxena U, Witte LD, Goldberg IJ: Release of endothelial cell lipoprotein lipase by plasma lipoproteins and free fatty acids. J Biol Chem 264: 4349–4355, 1989PubMedGoogle Scholar
  9. 9.
    Saxena U, Goldberg IJ: Interaction of lipoprotein lipase with glycosaminoglycans and apolipoprotein CII: effects of free fatty acids. Biochim Biophys Acta 1043: 161–168, 1990PubMedCrossRefGoogle Scholar
  10. 10.
    Saxena U, Witte LD, Goldberg IJ: Tumor necrosis factor induced release of endothelial cell lipoprotein lipase. Arteriosclerosis 10: 470–476, 1990Google Scholar
  11. 11.
    Peterson J, Bihain BE, Bengtsson-Olivecrona G, Deckelbaum RJ, Carpentier YA, Olivecrona T: Fatty acid control of lipoprotein lipase: a link between energy metabolism and lipid transport. Proc Natl Acad Sci USA 87: 909–913, 1990PubMedCrossRefGoogle Scholar
  12. 12.
    Kryski A, Kenno KA, Severson DL: Stimulation of lipolysis in rat heart myocytes by isoproterenol. Am J Physiol 248: H208–H216, 1985PubMedGoogle Scholar
  13. 13.
    Ramirez I, Kryski AJ, Ben-Zeev O, Schotz MC, Severson DL: Characterization of triacylglycerol hydrolase activities in isolated myocardial cells from rat heart. Biochem J 232: 229–236, 1985PubMedGoogle Scholar
  14. 14.
    Shimada K, Gill PJ, Silbert JE, Douglas WHJ, Fanburg BL: Involvement of cell heparan sulfate in the binding of lipoprotein lipase to cultured bovine endothelial cells. J Clin Invest 68: 995–1002, 1981PubMedCrossRefGoogle Scholar
  15. 15.
    Cisar LA, Hoogerwerf AJ, Cupp M, Rapport CA, Bensadoun A: Secretion and degradation of lipoprotein lipase in cultured adipocytes. J Biol Chem 264: 1767–1774, 1989PubMedGoogle Scholar
  16. 16.
    Chajek T, Stein O, Stein Y: Lipoprotein lipase of cultured mesenchymal rat heart cells. Hydrolysis of labelled very low density lipoprotein triacylglycerol by membrane-supported enzyme. Biochim Biophys Acta 528: 466–474, 1978Google Scholar
  17. 17.
    Friedman G, Chajek-Saul T, Stein O, Noe L, Etienne J, Stein Y: Beta-adrenergic stimulation enhanced translocation, processing and synthesis of lipoprotein lipase in rat heart cells. Biochim Biophys Acta 877: 112–120, 1986PubMedCrossRefGoogle Scholar
  18. 18.
    Pradines-Figueres, Vannier C, Ailhaud G: Lipoprotein lipase stored in adipocytes and muscle cells is a cryptic enzyme. J Lipid Res 31: 1467–1476, 1990PubMedGoogle Scholar
  19. 19.
    Friedman G, Stein O, Stein Y: Lipoprotein lipase of cultured mesenchymal rat heart cells. Modulation of enzyme activity by VLDL added to the culture medium. Biochim Biophys Acta 573: 521–534, 1979Google Scholar
  20. 20.
    Bengtsson G, Olivecrona T: Lipoprotein lipase. Mechanism of product inhibition. Eur J Biochem 106: 557–562, 19801980PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1992

Authors and Affiliations

  • Brian Rodrigues
    • 1
  • Michael R. Spooner
    • 1
  • David L. Severson
    • 1
  1. 1.Department of Pharmacology & Therapeutics Faculty of MedicineThe University of CalgaryCalgaryCanada

Personalised recommendations