Release of heart fatty acid-binding protein into plasma after acute myocardial infarction in man

  • Appie H. Kleine
  • Jan F. C. Glatz
  • Frans A. Van Nieuwenhoven
  • Ger J. Van der Vusse
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 8)


The release of cytoplasmic heart fatty acid-binding protein (H-FABP) into the plasma of cardiac patients up to 38 hr after the onset of the first clinical symptoms of acute myocardial infarction (AMI) was studied, using a sensitive direct and noncompetitive Enzyme Linked Immunosorbent Assay of the antigen capture type (sandwich ELISA), newly developed for the measurement of small amounts of human H-FABP in plasma samples. Plasma levels of H-FABP were compared with plasma activity levels of the myocardial cytoplasmic enzymes creatine kinase MB (CK-MB) and alpha-hydroxybutyrate dehydrogenase (α-HBDH). Upper normal levels of H-FABP (19μg/l), CK-MB (10 U/1) and α-HBDH (160 U/l) as determined in plasma from 72 blood donors served as threshold levels. H-FABP levels were significantly elevated above their threshold level within 3 hr after AMI. Peak levels of H-FABP, CK-MB and α-HBDH were reached 4.1 ± 0.9 hr, 8.4 ± 1.4 hr and 25.0 ± 9.5 hr (means ± S.D., n= 10) after acute myocardial infarction, respectively. Serial time curves of the plasma contents of H-FABP reveal that after myocardial infarction H-FABP is released in substantial amounts from human hearts. In 18 out of 22 patients with established AMI the plasma FABP level was at or above the threshold level in blood-samples taken within 3.5 hr after the first onset of symptoms of AMI, while for CK-MB this applied to 9 patients and for α-HBDH to 6 patients. These findings suggest that for an early indication of acute myocardial infarction in man cytoplasmic heart fatty acid-binding protein is more suitable than heart type creatine kinase MB and/or alpha-hydroxybutyrate dehydrogenase. (Mol Cell Biochem 116: 155–162, 1992)

Key words

fatty acid-binding protein ELISA acute myocardial infarction 


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  1. 1.
    Glatz JFC, Van der Vusse GJ: Cellular fatty acid-binding proteins: Current concepts and future directions. Mol Cell Biochem 98: 237–251, 1990PubMedGoogle Scholar
  2. 2.
    Bass NM: The cellular fatty acid binding proteins: Aspects of structure, regulation, and function. Int Rev Cytol 111: 143–184, 1988PubMedCrossRefGoogle Scholar
  3. 3.
    Veerkamp JH, Peeters RA, Maatman RGHJ: Structural and functional features of different types of cytoplasmic fatty acid-binding proteins. Biochim Biophys Acta 1081: 1–24, 1991PubMedCrossRefGoogle Scholar
  4. 4.
    Glatz JFC, van Bilsen M, Paulussen RJA, Veerkamp JH, van der Vusse GJ, Reneman RS: Release of fatty acid-binding protein from isolated rat heart subjected to ischemia and reperfusion or to the calcium paradox. Biochim Biophys Acta 961: 148–152, 1988PubMedCrossRefGoogle Scholar
  5. 5.
    Knowlton AA, Apstein CS, Saouf R, Brecher P: Leakage of heart fatty acid binding protein with ischemia and reperfusion in the rat. J Mol Cell Cardiol 21: 577–583, 1989PubMedCrossRefGoogle Scholar
  6. 6.
    Knowlton AA, Burner RE, Brecher P: Rabbit heart fatty acid-binding protein: Isolation, characterization, and application of a monoclonal antibody. Circ Res 65: 981–998, 1989PubMedCrossRefGoogle Scholar
  7. 7.
    Tanaka T, Hirota Y, Sohmiya K-I, Nishimura S, Kawamura K: Serum and urinary human heart fatty acid-binding protein in acute myocardial infarction. Clin Biochem 24: 195–201, 1991PubMedCrossRefGoogle Scholar
  8. 8.
    Ingelfinger JA, Mosteller F, Thibodeau LA, Ware JH. In: Bio-statistics in clinical medicine. MacMillan Publishing Co., Inc., New York, 1983, pp 85–113Google Scholar
  9. 9.
    Kleine AH, Glatz JFC, van Nieuwenhoven FA, Vallinga MIJ, Salden MHL, Bosman FT, Boersma WJA, Zegers ND, Van der Vusse GJ: Type-specific immunodetection of human heart fatty acid-binding protein with polyclonal anti-peptide antibodies. Mol Cell Biochem 98: 41–48, 1990PubMedCrossRefGoogle Scholar
  10. 10.
    Glatz JFC, Veerkamp JH: A radiochemical procedure for the assay of fatty acid binding by proteins. Anal Biochem 132: 89–95, 1983PubMedCrossRefGoogle Scholar
  11. 11.
    Wilson MB, Nakane PK. In: W Knapp, K. Holubar, G Wick (eds) Immunofluorescence and Related Techniques. Elsevier/North-Holland, Amsterdam, 1978, pp 215–224Google Scholar
  12. 12.
    Börchers T, Unterberg C, Riidel H, Robenek H, Spener F: Subcellular distribution of cardiac fatty acid-binding protein in bovine heart muscle and quantitation with an enzyme-linked immunosorbent assay. Biochim Biophys Acta 1002: 54–61, 1989PubMedCrossRefGoogle Scholar
  13. 13.
    Rosalki SB, Wilkinson JH: Reduction of alpha-ketobutyrate by human serum. Nature 188: 1110–1111, 1960PubMedCrossRefGoogle Scholar
  14. 14.
    Peeters RA, Veerkamp JH, Kanda T, Ono T, Van Kessel AD: Cloning of the cDNA encoding human skeletal muscle fatty acid-binding protein, its peptide sequence and chromosomal localization. Biochem J 276: 203–207, 1991PubMedGoogle Scholar
  15. 15.
    Waldman TA, Strauber W, Mogielnicki RP: The renal handling of low molecular weight proteins. II. Disorders of serum protein catabolism in patients with proteinuria, the nephrotic syndrome, or uremia. J Clin Invest 51: 2162–2174, 1972Google Scholar
  16. 16.
    Hall CL, Hardwicke J: Low molecular weight proteinuria. Ann Rev Med 30: 199–211, 1979PubMedCrossRefGoogle Scholar
  17. 17.
    Klocke FJ, Copley DP, Krawczyk JA, Reichlin M: Rapid renal clearance of immunoreactive canine plasma myoglobin. Circulation 65: 1522–1528, 1982PubMedCrossRefGoogle Scholar
  18. 18.
    Stone MJ, Waterman MR, Harimoto D, Murray G, Wilson N, Platt MR, Blomqvist G, Willerson JT: Serum myoglobin level as diagnostic test in patients with acute myocardial infarction. Br Heart J 39: 375–380, 1977PubMedCrossRefGoogle Scholar
  19. 19.
    Varki AP, Roby DS, Watts H, Zatuchni J: Serum myoglobin in acute myocardial infarction: A clinical study and review of the literature. Am Heart J 96: 680–688, 1978PubMedCrossRefGoogle Scholar
  20. 20.
    Drexel H, Dworzak E, Kirchmair W, Milz MM, Puschendorf B, Dienstl F: Myoglobinemia in the early phase of acute myocardial infarction. Am Heart J 105: 642–651, 1983PubMedCrossRefGoogle Scholar
  21. 21.
    McComb JM, McMaster EA, MacKenzie G, Adgey AAJ: Myoglobin and creatine kinase in acute myocardial infarction. Br Heart J 51: 189–194, 1984PubMedCrossRefGoogle Scholar
  22. 22.
    Isakov A, Shapira I, Burke M, Almog C: Serum myoglobin levels in patients with ischemic myocardial insult. Arch Intern Med 148: 1762–1765, 1988PubMedCrossRefGoogle Scholar
  23. 23.
    Ellis AK, Saran BR: Kinetics of myoglobin release and prediction of myocardial depletion after coronary artery reperfusion. Circulation 80: 676–683, 1989PubMedCrossRefGoogle Scholar
  24. 24.
    Ohman EM, Casey C, Bengtson JR, Pryor D, Tormey W, Horgan JH: Early detection of acute myocardial infarction: additional diagnostic information from serum concentrations of myoglobin in patients without ST elevation. Br Heart J 63: 335–338, 1990PubMedCrossRefGoogle Scholar
  25. 25.
    Driessen-Kletter MF, Amelink GJ, Bar PR, van Gijn J: Myoglobin is a sensitive marker of increased muscle membrane vulnerability. J Neurol 237: 23–238, 1990CrossRefGoogle Scholar
  26. 26.
    Sylvén C, Jansson E, Böör K: Myoglobin content in human skeletal muscle and myocardium: relation to fibre size and oxidative capacity. Cardiovasc Res 18: 443–446, 1984PubMedCrossRefGoogle Scholar
  27. 27.
    Lin L, Sylvén C, Sotonyi P, Somogyi E, Kaijser L, Jansson E: Myoglobin content and citrate synthase activity in different parts of the normal human heart. J Appl Physiol 69: 899–901, 1990PubMedGoogle Scholar
  28. 28.
    Paulussen RJA, Van Moerkerk HTB, Veerkamp JH: Immunochemical quantitation of fatty acid-binding proteins. Tissue distribution of liver and heart FABP types in human and porcine tissues. Int J Biochem 22: 393–398, 1990Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1992

Authors and Affiliations

  • Appie H. Kleine
    • 1
  • Jan F. C. Glatz
    • 1
  • Frans A. Van Nieuwenhoven
    • 1
  • Ger J. Van der Vusse
    • 1
  1. 1.Department of Physiology, Cardiovascular Research Institute MaastrichtUniversity of LimburgMaastrichtThe Netherlands

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