Characterization of Beta-Adrenoceptors in Sinoatrial Node and Left Ventricular Myocardium of Diabetic Rat Hearts by Quantitative Autoradiography

  • K. Saito
  • A. Kuroda
  • H. Tanaka
Part of the Developments in Cardiovascular Medicine book series (DICM, volume 130)


Although it has been reported that the response of catecholamines to myocardial contractility and heart rate are reduced in diabetic animals (1–6), very little is known about the beta-adrenoceptors in the conduction system of diabetic animals (7). We characterized beta-adrenoceptors in sinoatrial node (SA) and left ventricle of diabetic rat hearts by quantitiative autoradiography.


Diabetic Animal Diabetic Heart Sinoatrial Node Quantitative Autoradiography Autoradiographic Localization 
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  1. 1.
    Foy JM and Lucas PD. Effect of experimental diabetes, food deprivation and genetic obesity on the sensitivity of pithed rats to autonomic agents. Br J Pharmacol 1976:57:229–234.PubMedCrossRefGoogle Scholar
  2. 2.
    Fein FS, Kornstein LB, Strobeck JE, Capasso JM and Sonnenblick EH. Altered myocardial mechanics in diabetic rats. Circ Res 1980;47:922–933.PubMedCrossRefGoogle Scholar
  3. 3.
    Penpargkul S, Schaible T, Yipintsoi T and Scheuer J. The effect of diabetes on performance and metabolism of rat hearts. Circ Res 1980;47:911–921.PubMedCrossRefGoogle Scholar
  4. 4.
    Heyliger C, Pierce G, Singal P, Beamish R and Dhalla N. Cardiac alpha-and beta-adrenergic receptor alterations in diabetic cardiomyopathy. Basic Res Cardiol 1982;77:610–618.PubMedCrossRefGoogle Scholar
  5. 5.
    Vadlamudi R, Rodgers R and McNeill J. The effect of chronic alloxan and streptozotocin-induced diabetes on isolated rat heart performance. Can J Physiol Pharmacol 1982;60:902–911.PubMedCrossRefGoogle Scholar
  6. 6.
    Sunagawa R, Murakami K and Mimura G. Effects of adrenergic drugs on isolated and perfused hearts of streptozotocin-induced diabetic rats. Japan J Pharmacol 1987;44:233–240.CrossRefGoogle Scholar
  7. 7.
    Saito K, Kuroda A and Tanaka H. Characterization of beta-1 and beta-2-adrenoceptor subtypes in the atrioventricular node of diabetic rat hearts by quantitative autoradiography. Submitted for publication.Google Scholar
  8. 8.
    Saito K, Torda T, Potter WZ and Saavedra JM. Characterization of beta-1-and beta-2-adrenoceptor subtypes in the rat sinoatrial and stellate ganglia by quantitative autoradiography. Neuroscience Lett 1989;96:35–41.CrossRefGoogle Scholar
  9. 9.
    Karnovsky MJ. The localization of cholinesterase activity in rat cardiac muscle by electron microscopy. J Cell Biol 1964;23:217–223.PubMedCrossRefGoogle Scholar
  10. 10.
    Lipe S and Summers RJ. Autoradiographic analysis of the distribution of beta-adrenoceptors in the dog splenic vasculature. Br J Pharmac 1986;87:603–609.CrossRefGoogle Scholar
  11. 11.
    Saito K, Kurihara M, Cruciani R, Potter WZ and Saavedra JM. Characterization beta1-and beta2-adrenoceptor subtypes in the rat atrioventricular node by quantitative autoradiography. Cir Res 1988;62:173–177.CrossRefGoogle Scholar
  12. 12.
    Dooley DJ, Bittiger H and Reymann NC CGP 20712 A: a useful tool for quantitating beta1-and beta2-adrenoceptors. Eur J Pharmacol 1986;130:137–139.PubMedCrossRefGoogle Scholar
  13. 13.
    Molennar P, Canale E and Summers RJ. Autoradiographic localization of beta-1 and beta-2 adrenoceptors in guinea pig atrium and regions of the conducting system. J Pharmacol Exp Ther 1987;241:1048–1064.Google Scholar
  14. 14.
    Saito K, Potter WZ and Saavedra JM. Quantitative autoradiography of beta-adrenoceptors in the cardiac vagus ganglia of the rat. Eur J Pharmacol 1988;153:289–293.PubMedCrossRefGoogle Scholar
  15. 15.
    Pinto JEB, Nazarali AJ, Torda T and Saavedra JM. Autoradiographic characterization of beta-adrenoceptors in rat heart valve leaflets. Am J Physiol 1989;256:H821–827.Google Scholar
  16. 16.
    Savarese JJ and Berkowitz BA. Beta-adrenergic receptor decrease in diabetic rat hearts. Life Sci 1979;25:2075–2078.PubMedCrossRefGoogle Scholar
  17. 17.
    Williams RS, Schaible TF, Scheuer J and Kennedy R. Effects of experimental diabetes of adrenergic and cholinergic receptors of rat myocardium. Diabetes 1983;32:881–886.PubMedCrossRefGoogle Scholar
  18. 18.
    Sundaresan PR, Sharma VK, Gingold SI and Banerjee SP. Decreased beta-adrenergic receptors in rat streptozotocin-induced diabetes: Role of thyroid hormone. Endocrinology 1984;114:1358–1363.PubMedCrossRefGoogle Scholar
  19. 19.
    Atkins FL, Dowell RT and Love S. Beta-adrenergic receptors, adenylate cyclase activity and cardiac dysfunction in the diabetic rat. J Cardiovasc Pharmacol 1985;7:66–70.PubMedCrossRefGoogle Scholar
  20. 20.
    Nishio Y, Kashiwagi A, Kida Y, Kodama M, Abe N, Saeki Y and Shigeta Y. Deficiency of cardiac beta-adrenergic receptor in streptozotocin-induced diabetic rats. Diabetes 1988;1181–1187.Google Scholar
  21. 21.
    Senges J, Brachmann J, Pelzer D, Hasslacher C, Weiher E and Kubler W. Altered cardiac automaticity and conduction in experimental diabetes mellitus. J Mol Cell Cardiol 1980;12:1341–1351.PubMedCrossRefGoogle Scholar
  22. 22.
    Kuroda A, Saito K and Tanaka H. Histochemical studies of conduction system of diabetic rat hearts. Arch Histol Cytol. In press.Google Scholar

Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • K. Saito
    • 1
  • A. Kuroda
    • 2
  • H. Tanaka
    • 2
  1. 1.Health Service Center of National Institute of Fitness and Sports in KanoyaJapan
  2. 2.First Department of Internal Medicine of Faculty of MedicineKagoshima UniversityKagoshimaJapan

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