Abstract
Sequential extraction procedures using methanol or methanol followed by acetone-water (1:1) (v/v) were used to measure condensed tannins in pine litter. Tannins in the extracts and residues were quantified by the amount of anthocyanidins formed in n-butanol-HCl. The amount of tannin extracted varied with the solvent and the number of extractions, but the total amount of tannin measured in extracts and residues was not significantly different for any of the sequences. An extraction sequence consisting of two methanol extractions followed by one acetone-water extraction was selected to measure tannins in pine litter. When the anthocyanidin procedure was used to measure condensed tannins from southern pine foliage, 5 percent HC1 in the n-butanol was the optimal acid concentration. The yield of anthocyanidin was increased by 62 percent when 12 mg/L of ferrous iron was added to the n-butanol-HCl. The sequential extraction method was used to follow the loss of tannin from litter bags containing decomposing shortleaf pine (Pinus echinata Mill.) senescent foliage. After one season, 16 percent of the original 73.9 g/kg of tannin was detectable. After two seasons, the litter contained 5.8 g/kg (8 percent) of the original tannin content.
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Tiarks, A.E., Meier, C.E., Flagler, R.B., Steynberg, E.C. (1992). Sequential Extraction of Condensed Tannins from Pine Litter at Different Stages of Decomposition. In: Hemingway, R.W., Laks, P.E. (eds) Plant Polyphenols. Basic Life Sciences, vol 59. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3476-1_33
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DOI: https://doi.org/10.1007/978-1-4615-3476-1_33
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