The Hamster Cheek Pouch Model of Carcinogenesis and Chemoprevention
The Syrian golden hamster cheek pouch carcinogenesis model is probably the best-known animal system for studying events involved in the development of premalignancy and malignancy in human oral cancer. Like the mouse skin model of carcinogenesis, the hamster cheek pouch model provides data on the effect of carcinogens, promoters, and chemopreventive agents, which makes both these models very useful in selecting chemopreventive agents for human cancer intervention studies (1–8). Our first approach to understanding the important early events that occur in the hamster cheek pouch model of carcinogenesis was to compare this model with the mouse skin model, in which a number of critical events have already been well characterized (9–11). The carcinogenesis studies in the hamster cheek pouch were carried out using multiple applications of 7,12-dimethylbenz[a] an thracene (DMBA) (0.5% in mineral oil, 3 times a week). This protocol induced a hyperplastic response in the pouch epithelium after only a few applications, followed by the appearance after 6–8 weeks of treatment of a variety of dysplastic lesions resembling human premalignant lesions. Benign and malignant tumors (papillomas and squamous cell carcinomas [SCCs]) started to develop after 10 weeks of treatment (12,13).
KeywordsBenzoyl Peroxide Mouse Skin Chemopreventive Agent Nucleolar Organizer Region Oral Carcinogenesis
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