Effect of Herpes Simplex Virus Type 1 Infection on Cytokine Gene Expression in Activated Murine Peritoneal Macrophages
The intrinsic resistance to herpes simplex virus type 1 (HSV-1) of murine peritoneal macrophages (PMø) obtained after in vivo infection of different stimuli has been investigated and shown to vary depending on the state of Mø activation. Activation of Mø by C. parvum (CP-Mø) or by an avirulent strain of S. typhimurium (Sal-Mø) increased the permissiveness of Mø to HSV-1 infection as evidenced by increased HSV-1 immediate early (IE) gene expression, synthesis of IE proteins, and the degree of cytopathic effect. HSV-1 infection was also found to sharply reduce the level of IL-1-β mRNA in CP-Mø) and Sal-Mø, and the level of IL-β mRNA in infected Sal-Mø, as measured by northern blot hybridization. Barely detectable levels of IL-β mRNA were found in Sal-Mø after infection with HSV-1 when the polymerase chain reaction (PCR) assay was used to confirm the reduction of IL-1-β mRNA. These data suggest that HSV-1 infection can modulate gene expression of some cytokines in the activated Mø.
KeywordsPeritoneal Macrophage Intrinsic Resistance Northern Blot Hybridization Cytokine Gene Expression Peritoneal Exudate Cell
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