Potassium-Stimulated Release of Taurine in a Crude Retinal Preparation Obtained from the Rat is Calcium Independent
The role of taurine as a potential neurotransmitter in the retina has been questioned in numerous literature reports. Thus, crude retinal homogenates prepared from rat retina were preloaded with [3H]taurine under either high-or low-affinity uptake conditions and were then subjected to superfusion techniques to determine whether KC1 (56 mM), veratridine (100 μM), 4-aminopyridine (1 mM), or LiC1 (56 mM3) could evoke the release of [3H]taurine. Dependence of the K+-evoked release of [3H]taurine on Ca2+ was also measured since one of the essential criteria for a neurotransmitter is Ca2+-dependent release of the substance from presynaptic nerve terminals.
KeywordsLithium Chloride Scorpion Venom Cortex Slice Taurine Release Presynaptic Nerve Terminal
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- 6.M.P. Blaustein, Effects of potassium, veratridine, and scorpion venom on calcium accumulation and transmitter release by nerve terminals in vitro, J. Physiol. (London) 47:617–655 (1975).Google Scholar
- 9.H. Pasantes-Morales, J. Morán, and A. Schousboe, Taurine release associated to cell swelling in the nervous system, in:“Taurine: Functional Neurochemistry, Physiology, and Cardiology,” H. Pasantes-Morales, D.L. Martin, W. Shain, and R. Martín del Rio, eds., Wiley-Liss, New York, pp. 369–376 (1990).Google Scholar