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Flavonoid Synthesis in Petunia Hybrida; Genetics and Molecular Biology of Flower Colour

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Part of the book series: Recent Advances in Phytochemistry ((RAPT,volume 26))

Abstract

Except for yellow colours due to carotenoids, the major flower pigments are flavonoids, more precisely anthocyanins and flavonol glycosides. Under natural conditions, coloured flowers attract pollinators and, as such, flavonoids can be considered to perform a vital function in the life cycle of the plant. Besides contributing to floral pigmentation, flavonoids have been shown to play a role in a number of phenomena: defence against phytopathogens1,2 and predators3 and nodule induction in the Rhizobium-legume symbiosis.4,5,6 Because flavonoids are phenolic compounds they can act as metal chelators and antioxidants, and because they are aromatic compounds they might provide protection against damage by UV light. Flavonoids are widely used in medicine as therapeutic drugs, although they are also known to be causative agents of some diseases.7

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References

  1. Derno, R.F., McClure, M.S. 1983. Variable Plants And Herbivores in Natural and Managed Systems. Academic Press, New York.

    Google Scholar 

  2. Dixon, R.A. 1986. The phytoalexin response: elicitation signalling and control of host gene expession. Biol. Rev. 61:239–291.

    Article  CAS  Google Scholar 

  3. Hedin, P.A., Waage, S.K. 1986. In: Plant Flavonoids in Biology and Medicine, (V. Cody, E. Middleton, J.B. Harborne, eds.) Alan R. Liss Inc., New York, pp. 87–106.

    Google Scholar 

  4. Firmin, J.L., Wilson, K.E., Rossen, L., Johnston, A.W.B. 1986. Flavonoid activation of nodulation genes in Rhizobium reversed by other compounds present in plants. Nature 324:90–92.

    Article  CAS  Google Scholar 

  5. Peters, N.K., Frost, J.N., Long, S.R. 1986. A plant flavone, inteolin, induces expression of Rhizobium meliloti nodulation genes. Science 233:977–980.

    Article  PubMed  CAS  Google Scholar 

  6. Redmond, J.W., Batley, M., Djordjevic, M.A., Innes, M.W., Kuempel, P.W., Rolfe, B.G. 1986. Flavones induce expression of nodulation genes in Rhizobium. Nature 323, 632–635.

    Article  CAS  Google Scholar 

  7. Cody, V., Middleton, E., Harborne, J.B. 1986. Plant Flavonoids in Biology and Medicine: Biochemical, Pharmacological and Structure-Activity Relationships. Alan R. Liss Inc., New York.

    Google Scholar 

  8. Mendel, G. 1865. Versüche über Pflanzen-Hybriden. Verh. Naturf. Verein. Brünn, IV, 3–47.

    Google Scholar 

  9. Wheldale, M.W. 1909. The colours and pigments of flowers with special reference to genetics. Proc. Roy. Soc. B, 81:44–60.

    Article  Google Scholar 

  10. Wheldale, M.W. 1925. The anthocyanin pigments of plants. University Press, Cambridge.

    Google Scholar 

  11. Beadle, G.W., Tatum, E.C. 1941. Genetic control of biochemical reactions in Neurospora. Proc. Natl. Acad. Sci. USA, 27:499–506.

    Article  PubMed  CAS  Google Scholar 

  12. Vavilov, N.I. 1930. The Linnean series as a system. Proc. 5th Int. Bot. Cong. Cambridge. 213–216.

    Google Scholar 

  13. Bate-Smith, E.C. 1948. Paper chromatography of anthocyanins and related substances in petal extracts. Nature 161:835.

    Article  PubMed  CAS  Google Scholar 

  14. Hanson, K.R., Havir, E.A. 1972. The enzymatic elimination of ammonia. In: The Enzymes. (P.D. Boyer, ed.) Academic Press, New York, Vol. 7, pp. 577–625.

    Google Scholar 

  15. Hanson, K.R., Havir, E.A. 1981. Phenylalanine Ammonia Lyase. In: The Biochemistry of Plants. (P.K. Stumpf, E.E. Conn, eds.) Academic Press, New York, Vol 7, pp. 577–625.

    Google Scholar 

  16. Nair, P.M., Vining, L.C. 1965. Cinnamic acid hydroxylase in spinach. Phytochemistry 4:161–168.

    Article  CAS  Google Scholar 

  17. Russell, D.W., Conn, E.E. 1967. The cinnamic acid 4-hydroxylase of pea seedlings. Arch. Biochem. Biophys. 122:256–258.

    Article  PubMed  CAS  Google Scholar 

  18. Hahlbrock, K., Grisebach, H. 1970. Formation of coenzyme A esters of cinnamic acid with an enzyme preparation from cell suspension cultures of parsley. FEBS Lett. 11:62–64.

    Article  PubMed  CAS  Google Scholar 

  19. Walton, E., BUTT, V.S. 1970. The activation of cinnamate by an enzyme from leaves of spinach beet (Beta vulgaris L. ssp. vulgaris). J. Exp. Bot. 21:887–891.

    Article  CAS  Google Scholar 

  20. Knobloch, K.H., Hahlbrock, K 1975. Isoenzymes of pCoumarate: CoA ligase from cell suspension cultures of Glycine max. Eur. J. Biochem. 52:311–320.

    Article  PubMed  CAS  Google Scholar 

  21. Wallis, P.J., Rhodes, M.J.C. 1977. Multiple forms of hydroxycinnamate:CoA ligase in etiolated pea seedlings. Phytochemistry 16:1891–1894.

    Article  CAS  Google Scholar 

  22. Butt, V.S., Wilkinson, E.M. 1979. In: Regulation of Secondary Product and Plant Hormone Metabolism. (M. Luckner, K. Schreiber, eds.) Pergamon Press, Oxford, Vol. 55, pp. 147–154.

    Google Scholar 

  23. Ranjeva, R., Faggion, R., Boudet, A. 1975. Physiol. Veg. 13:725–734.

    CAS  Google Scholar 

  24. Ranjeva, R., Boudet, A., Faggion, R. 1976. Phenolic metabolism in petunia tissues. IV. Properties of p-coumarate: coenzyme A ligase isoenzymes. Biochimie 58:1255–1262.

    Article  PubMed  CAS  Google Scholar 

  25. Ranjeva, R., Boudet, A.M., Alibert, G. 1979. In: Regulation of Secondary Product and Plant Hormone Metabolism. (M. Luckner, K. Schreiber, eds.) Pergamon Press, Oxford, Vol. 55, pp. 91–100.

    Google Scholar 

  26. Ebel, J., Hahlbrock, K. 1982. Biosynthesis. In: The Flavonoids, Advances in Research. (J.B. Harborne, T.J. Mabry, eds.) Chapman and Hall, London, pp. 641–679.

    Google Scholar 

  27. Heller, W. 1986. Flavonoid biosynthesis, an overview. In: Plant Flavanoids in Biology and Medicine. (V. Cody, E. Middleton, J.B. Harborne, eds.) Alan R. Liss, New York, pp. 25–42.

    Google Scholar 

  28. Heller, W., Forkmann, G. 1988. Biosynthesis. In: The Flavonoids. (J.B. Harborne, ed.) Chapman and Hall, London, pp.399–425.

    Google Scholar 

  29. Spribille, R., Forkmann, G. 1981. Genetic control of chalcone synthase activity in flowers of Matthiola incana R.Br. Z. Naturforsch. 36c:619–624.

    CAS  Google Scholar 

  30. Spribille, R., Forkmann, G. 1982. Genetic control of chalcone synthase activity in flowers of Antirrhinum majus. Phytochemistry 21:2231–2234.

    Article  CAS  Google Scholar 

  31. Whitehead, J.M., Dixon, R.A. 1983. Chalcone synthase from cell suspension cultures of Phaseolus vulgaris L. Biochim. Biophys. Acta 747:298–303.

    Article  CAS  Google Scholar 

  32. Stotz, G., Spribille, R., Forkmann, G. 1984. Flavonoid synthesis in flowers of Verbena hybrida. J. Plant Physiol. 116:173–183.

    Article  CAS  Google Scholar 

  33. Kamsteeg, J., Van Brederode, J., Verschuren, P.M., Van Nigtevecht, G. 1981. Z. Pflanzenphysiol. 102:435.

    CAS  Google Scholar 

  34. Dooner, H.K. 1983. Co-ordinate genetic regulation of flavonoid biosynthetic enzymes in maize. Mol. Gen. Genet. 189:136–141.

    Article  CAS  Google Scholar 

  35. Sommer, H., Saedler, H. 1986. Structure of the chalcone synthase gene of Antirrhinum majus. Mol. Gen. Genet. 202:429–434.

    Article  CAS  Google Scholar 

  36. Reif, H.J., Niesbach, U., Deumling, B., Saedler, H. 1985. Cloning and analysis of two genes for chalcone synthase from Petunia hybrida. Mol. Gen. Genet. 199:208–215.

    Article  CAS  Google Scholar 

  37. Koes, R.E., Spelt, C.E., Mol, J.N.M., Gerats, A.G.M. 1987. The chalcone synthase multigene family of Petunia hybrida: sequence homology, chromosomal localization and evolutionary aspects. Plant Mol. Biol. 10:159–169.

    Article  CAS  Google Scholar 

  38. Hermann, A., Schulz, W., Hahlbrock, K. 1988. Two alleles of the single-copy chalcone synthase gene in parsley differ by a transposon-like element. Mol. Gen. Genet. 212:93–98.

    Article  Google Scholar 

  39. Wienand, U., Weydemann, U., Niesbach-Kloesgen, U., Peterson, P.A., Saedler, H. 1986. Molecular cloning of the C2 locus ofZea maysthe gene coding for chalcone synthase. Mol. Gen. Genet. 203:202–207.

    Article  CAS  Google Scholar 

  40. Niesbach-Kloesgen, U., Barzen, E., Bernhardt, J., Rohde, W., Schwarz-Sommer, Z.S., Reif, H.J., Wienand, U., Saedler, H. 1987. Chalcone synthase genes in plants: a tool to study evolutionary relationships. J. Mol. Evol. 26:213–225.

    Article  CAS  Google Scholar 

  41. Ryder, T.B., Hedrick, S.A., Bell, J.N., Liang, X., Clouse, S.D., Lamb, C.J. 1987. Organization and differential activation of a gene family encoding the plant defense enzyme chalcone synthase in Phaseolus vulgaris. Mol. Gen. Genet. 210:219–233.

    Article  PubMed  CAS  Google Scholar 

  42. Feinbaum, R.L., Ausubel, F.M. 1988. Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene. Mol. Cell. Biol. 8:1985–1992.

    PubMed  CAS  Google Scholar 

  43. Harder, R., Marheineke, J. 1935. Weitere untersuchungen über die Musterbildung an Petunienblüten. Nachr. Biol. 2:97–105.

    Google Scholar 

  44. Harder, R. 1938. Ueber Farb-und Muster änderungen bei Blüten. Naturwissenschaften 26:713–728.

    Article  Google Scholar 

  45. Mol J.N.M., Schram, A.W., De Vlaming, P., Gerats, A.G.M., Kreuzaler, F., Hahlbrock, K., Reif, H.J., Veltkamp, E. 1983. Regulation of flavonoid gene expression in Petunia hybrida: description and partial characterization of a conditional mutant in chalcone synthase gene expression. Mol. Gen. Genet. 192:424–429.

    Article  CAS  Google Scholar 

  46. Koes, R.E., Spelt, C.E., Reif, H.J., Van Den Elzen, P., Veltkamp, E., Mol, J.N.M. 1986. Floral tissue of Petunia hybrida (V30) expresses only one member of the chalcone synthase multigene family. Nucl. Acids Res. 14:5229–5239.

    Article  PubMed  CAS  Google Scholar 

  47. Mol, J.N.M., Robbins, M.P., Dixon, R.A., Veltkamp, E. 1985. Spontaneous and enzymic rearrangement of naringenin chalcone to flavanone. Phytochemistry 24:2267–2269.

    Article  CAS  Google Scholar 

  48. Chmiel, E., Suetfeld, R., Wiermann, R. 1983. Conversion of phlorolgucinol-type chalcones by purified chalcone isomerase from Tulip anthers and from Cosmos petals. Biochem. Physiol. Pflanzen 178:139–146.

    CAS  Google Scholar 

  49. Kuhn, B., Forkmann, G., Seyffert, W. 1978. Genetic control of chalcone flavanone isomerase activity in Callistephus chinensis. Planta 138:199–203.

    Article  CAS  Google Scholar 

  50. Forkmann, G., Dangelmayer, B. 1980. Genetic control of chalcone isomerase activity in flowers of Dianthus caryophyllus. Biochem. Genet. 18:519–527.

    Article  PubMed  CAS  Google Scholar 

  51. Mehdy, M.C., Lamb, C.J. 1987. Chalcone isomerase cDNA cloning and mRNA induction by fungal elicitor, wounding and infection. EMBO J. 6:1527–1533.

    PubMed  CAS  Google Scholar 

  52. Van Tunen, A.J., Koes, R.E., Spelt, C.E., Van Der Krol, A.R., Stuitje, A.R., Mol, J.N.M. 1988. Cloning of the two chalcone flavanone isomerase genes from Petunia hybrida: Coordinate, light regulated and differential expression of flavonoid genes. EMBO J. 7:1257–1263.

    PubMed  Google Scholar 

  53. Van Tunen, A.J., Hartman, S.A., Mur, L.A., Mol, J.N.M. 1989. Regulation of chalcone flavanone isomerase (CHI)gene expression in Petunia hybrida: The use of alternative promoters in corolla, anthers and pollen. Plant Mol. Biol. 12:539–551.

    Article  Google Scholar 

  54. Forkmann, G., Kuhn, B. 1979. Genetic control of chalcone isomerase activity in anthers of Petunia hybrida. Planta 144:189–192.

    Article  CAS  Google Scholar 

  55. Van Weely, S., Bleumer, A., Spruyt, R., Schram, A.W. 1983. Chalcone isomerase in flowers of mutants of Petunia hybrida. Planta 159:226–230.

    Article  Google Scholar 

  56. Van Tunen, A.J., Mur, L.A., Brouns, G.S., Rienstra, J.D., Koes, R.E., Mol, J.N.M. 1990. Pollen-and anther-specific chi promotors from petunia: Tandem promoter regulation of the chiA gene. Plant Cell 2:393–401.

    PubMed  Google Scholar 

  57. Van Tunen, A.J., Mur, L.A., Recourt, K., Gerats, A.G.M., Mol, J.N.M. 1991. Regulation and manipulation of flavonoid gene expression in anthers of Petunia: the molecular basis of the Po mutation. Plant Cell 3:39–48.

    PubMed  Google Scholar 

  58. Fritsch, H., Grisebach, H., 1975. Biosynthesis of cyanidin in cell cultures of Haplopappus gracilis. Phytochemistry 14:2437–2442.

    Article  CAS  Google Scholar 

  59. Doodeman, M., Tabak, A.J.H., Schram, A.W., Bennink, GJ.H. 1982. Hydroxylation of cinnamic acids and flavonoids during biosynthesis of anthocyanins in Petunia hybrida Hort. Planta 154:546–549.

    Article  CAS  Google Scholar 

  60. Froemel, S., De Vlaming, P., Stotz, G., Wiering, H., Forkmann, G., Schram, A.W. 1985. Genetic and biochemical studies on the conversion of flavanones to dihydroflavonols in flowers of Petunia hybrida. Theor. Appl. Genet. 70:561–568.

    Article  CAS  Google Scholar 

  61. Britsch, L.H., Grisebach, H. 1986. Purification and characterization of (2S) flavanone 3-hydroxylase from Petunia hybrida. Eur. J. Biochem. 135:569–577.

    Article  Google Scholar 

  62. Forkmann, G., Stotz, G. 1981. Genetic control of flavanone 3hydroxylase and flavonoid 3’-hydroxylase activity in Antirrhinum majus (Snapdragon). Z. Naturforsch. 36C:411–416.

    CAS  Google Scholar 

  63. Forkmann, G., Stotz, G. 1984. Selection and characterisation of flavanone 3-hydroxylase mutants in Dahlia, Streptocarpus, Verbena and Zinnia. Planta 161:261–265.

    Article  CAS  Google Scholar 

  64. Forkmann, G. 1989. Gene-enzyme relations and genetic manipulation of anthocyanin biosynthesis in flowering plants. In: The Genetics of Flavonoids. (E.D. Styles, G.A. Gavazzi, M.L. Racchi, eds.) Edizioni Unicopli., Milan, pp. 49–60.

    Google Scholar 

  65. Larson, R.L. 1989. Genetics, precursors and enzymes in flavonoid biosynthesis in maize. In: The Genetics of Flavonoids. (E.D. Styles, G.A. Gavazzi, M.L. Racchi, eds.) Edizioni Unicopli., Milan. pp. 7178.

    Google Scholar 

  66. Martin, C., Prescott, A., Mackay, S., Bartlett, J., Vrijlandt, E. 1991. Control of anthocyanin biosynthesis in flowers of Antirrhinum majus. THe Plant Journal 1:37–49

    Article  PubMed  CAS  Google Scholar 

  67. Wiering, H. 1974. Genetics of flower colour in Petunia hybrida hort. Gen. Phaenen 17:117–134.

    CAS  Google Scholar 

  68. Tabak, A.J.H., Meyer, H., Bennink, G.J.H. 1978. Modification of the B-ring during flavonoid biosynthesis in Petunia hybrida: introduction of the 3’ hydroxyl group is regulated by the gene Htl. Planta 139:67–71.

    Article  CAS  Google Scholar 

  69. wiering, H., De Vlaming, P. 1984. Genetics of flower and pollen colours. In: Petunia. (K.C. Sink, ed.) Monographs on Theoretical and Applied Genetics, Springer Verlag, Berlin, pp. 49–67.

    Google Scholar 

  70. Stotz, G., De Vlaming, P., Wiering, H., Schram, A.W., Forkmann, G. 1985. Genetic and biochemical studies on flavonoid 3’-hydroxylation in flowers of Petunia hybrida. Theor. Appl. Genet. 70:300–305.

    Article  CAS  Google Scholar 

  71. Forkmann, G., Heller, W., Grisebach, H. 1980. Anthocyanin biosynthesis in flowers of Matthiola incana:flavanone 3- and flavonoid 3’-hydroxylases. Z. Naturforsch. 35c:691–695.

    CAS  Google Scholar 

  72. Stafford, H.A., Lester, H.H. 1982. Enzymatic and non-enzymatic reduction of (+) dihydroquercetin to its 3,4-diol. Plant Physiol. 70:695–698.

    Article  PubMed  CAS  Google Scholar 

  73. Stafford, H.A., Lester, H.H. 1984. Flavan-3-ol biosynthesis. The conversion of (+)-dihydroquercetin and flavan-3,4-cis-diol (leucoanthocyanidin) to (+)-catechin by reductases extracted from cell suspension cultures of Douglas fir. Plant Physiol. 76:184

    Article  PubMed  CAS  Google Scholar 

  74. Stafford, H.A., Lester, H.H. 1985. Flavan-3-ol biosynthesis. The conversion (+)-dihydroquercetin and its flavan-3,4-cis-diol (leucodelphinidin) and to (+)-gallocatechin by reductases extracted from tissue cultures of Ginkgo biloba and Pseudotsuga menziesii. Plant Physiol. 78:791–794.

    Article  PubMed  CAS  Google Scholar 

  75. Kristiansen, K.N. 1984. Biosynthesis of proanthocyanidins in barley: Genetic control of the conversion of dihydroquercetin to catechin and procyanidins. Carlsberg Res. Commun. 49:503–524.

    Article  CAS  Google Scholar 

  76. Kristiansen, K.N. 1986. Conversion of (+)-dihydroquercetin to (+)2,3-trans-3,4-cis-leucocyanidin and (+)catechin with an enzyme extract from maturing grains of barley. Carlsberg Res. Commun. 51:51–60.

    Article  Google Scholar 

  77. Martin, C., Carpenter, R., Sommer, H., Saedler, H., Coen, E.S. 1985. Molecular analysis of instability in flower pigmentation in Antirrhinum majus, following the isolation of the pallida locus by transposon tagging. EMBO J 4:1625–1630.

    PubMed  CAS  Google Scholar 

  78. Beld, M.G.H.M., Martin, C., Huits, H., Stuitje, A.R., Gerats A.G.M. 1989. Flavonoid synthesis in Petunia hybrida: partial characterisation of dihydroflavonol 4-reductase genes. Plant Mol. Biol. 13:491–502.

    Article  PubMed  CAS  Google Scholar 

  79. Forkmann, G., Ruhnau, B. 1987. Distinct substrate specificity of dihydroflavonol 4-reductase from flowers of Petunia hybrida. Z. Naturforsch. 42C:1146–1148.

    Google Scholar 

  80. Gerats, A.G.M., De Vlaming, P., Doodeman, M., AL, B., Schram, A.W. 1982. Genetic control of the conversion of dihydroflavonols into flavonols and anthocyanins in flowers of Petunia hybrida. Planta 155:364–368.

    Article  CAS  Google Scholar 

  81. Reddy, G.M., Britsch, L., Salamini, F., Saedler, H., Rohde, W. 1987. The Al (anthocyanin-1) locus in Zea mays encodes dihydroquercetin reductase. Plant Sci. 52:7–13.

    Article  CAS  Google Scholar 

  82. Meyer, P., Heidmann, I., Forkmann, G., Saedler, H. 1987. A new Petunia flower colour generated by transformation of a mutant with a maize gene. Nature 330:677–678.

    Article  PubMed  CAS  Google Scholar 

  83. Linn, F., Heidmann, I., Saedler, H., Meyer, P. 1990. Epigenetic changes in the expression of the maize Al gene in Petunia hybrida: Role of numbers of integrated gene copies and state of methylation. Mol. Gen. Genet. 222:329–336.

    Article  PubMed  CAS  Google Scholar 

  84. Heller, W., Britsch, L., forkmann, G., grisebach, H. 1985. Leucoanthocyanidins as intermediates in anthocyanidin biosynthesis in flowers of Matthiola incana R. Br. Planta 163:191–196.

    CAS  Google Scholar 

  85. Heller, W., Forkmann, G., Britsch, L., Grisebach, H. 1985. Enzymatic reduction of the (+)-dihydroflavnols to flavan-3,4-cis-diols with flower extracts from Matthiole incana and its role in anthocyanin biosynthesis. Planta 165:284–287.

    Article  CAS  Google Scholar 

  86. O’reilly, C., Shepherd, N.S., Pereira, A., Schwarzsommer, Z.S., Bertram, I., Robertson, D.S., Peterson, P.A., Saedler, H. 1985. Molecular cloning of the al locus of Zea mays using the transposable elements En and Mu. EMBO J. 44:877–882.

    Google Scholar 

  87. Coen, E.S., Carpenter, R., Martin, C. 1986. Transposable elements generate novel patterns of gene expression in Antirrhinum majus. Cell 47:285–296.

    Article  PubMed  CAS  Google Scholar 

  88. Bartlett, N.J.R. 1989. The genetic control of anthocyanin biosynthesis in Antirrhinum majus. Ph.D. thesis, U. of East Anglia, Norwich.

    Google Scholar 

  89. Reddy, A.R., Coe, E.H. 1962. Inter-tissue complementation: A simple technique for direct analysis of gene action sequence. Science 138:149–150.

    Article  PubMed  CAS  Google Scholar 

  90. Menssen, A., Hoehmann, S., Martin, W., Schanle, P.S., Peterson, P.A., Saedler, H., Gierl, A. 1990. The En/Spm transposable element of Zea mays contains splice sites at the termini generating a novel intron from a dSpm element in the A2 gene. EMBO J. 9:3051–3058.

    PubMed  CAS  Google Scholar 

  91. Larson, R.L., Coe, E.H. 1968. Enzymatic action of the Bz anthocyanin factor in maize. Proc. XII Int. Cong. Genet. 1:131.

    Google Scholar 

  92. Jonsson, L.M.V., Aarsman, M.E.G., Bastiaannet, J., Donker-Koopman, W., Gerats, A.G.M., Schram, A.W. 1984. Common identity of UDP-Glucose:anthocyanidin-3-0-glucosyltransferase and UDP-Glucose:flavonol-3-0-glucosyltransferase in flowers of Petunia hybrida. Z. Naturforsch. 39c:559–567.

    CAS  Google Scholar 

  93. Swain, T. 1976. Flavonoids. In: Chemistry and Biochemistry of Plant Pigments. (T.W. Goodwin, ed.) Academic Press, New York, Vol. 1, pp. 425–463.

    Google Scholar 

  94. Marty, F., Branton, D., leigh, R.A. 1980. Plant vacuoles. In: The Biochemistry of Plants. (P.K. Stumpf, E.E. Conn, eds.) Academic Press, New York, Vol. 1, pp. 625–658.

    Google Scholar 

  95. kho, K.F.F., Kamsteeg, J., Van Brederode, J. 1978. Identification, properties and genetic control of UDP Glucose: cyanidin 3-0-glucosyltransferase in Petunia hybrida. Z. Pflanzenphysiol. 88:449–464.

    CAS  Google Scholar 

  96. Gerats, A.G.M., Wallroth, M., Donker-Koopman, W., Groot, S., Schram, A.W. 1983. UDPGlucose: 3-0-flavonoid glucosyltransferase in flowers of Petunia hybrida. Theor. Appl. Genet. 65:349–352.

    Article  CAS  Google Scholar 

  97. Gerats, A.G.M., Vrijlandt, E., Wallroth, M., Schram, A.W. 1985. The influence of the genes Anl, An2 and An4 on the activity of the enzyme UDP glucose: flavonoid 3-o-glucosyltransferase in flowers of Petunia hybrida. Biochem. Genet. 23:591–598.

    Article  PubMed  CAS  Google Scholar 

  98. Larson, R.L., Coe, E.H. 1977. Gene-dependent flavonoid glucosyltransferase in maize. Biochem. Genet. 15:153–156.

    Article  PubMed  CAS  Google Scholar 

  99. Dooner, H.K., Nelson O.E. 1977. Genetic control of UDP-Glucose: flavonol 3-0-glucosyltransferase in the endosperm of maize. Biochem. Genet. 15:501–519.

    Article  Google Scholar 

  100. Teusch, M., Forkmann, G., Seyffert, W. 1986. UDPGlucose:anthocyanidin/flavonol 3-0-glucosyltransferase in enzyme preparation from flower extracts of genetically defined lines of Matthiola incana R.Br. Z. Naturforsch. 41c:699–706.

    Google Scholar 

  101. Griesbach, R.J., Asen, S. 1990. Characterization of the flavonol glycosides in Petunia. Plant Sci. 70:49–56.

    Article  CAS  Google Scholar 

  102. Jonsson, L.M.V., Aarsman, M.E.G., Van Diepen, J., De Vlaming, P., Schram, A.W. 1984. Properties and genetic control of anthocyanin 5-0-glucosyltransferase in flowers of Petunia hybrida. Planta 160:341–347.

    Article  CAS  Google Scholar 

  103. Teusch, M., Forkmann, G., Seyffert, W. 1986. Genetic control of UDP-glucose: anthocyanin 5-0-glucosyltransferase from flowers of Matthiola incana R. Br. Planta 168:586–591.

    CAS  Google Scholar 

  104. Kamsteeg, J., Van Brederode, J., Van Nigtevecht, G. 1978. Identification, properties and genetic control of UDP-Glucose: Cyanidin 3-rhamnosyl (1–6)-glucoside, 5-0-glucosyl-transferase isolated from petals of the Red Campion (Silene dioica). Biochem. Genet. 16:1059–1071.

    Article  PubMed  CAS  Google Scholar 

  105. Kamsteeg, J., Van Brederode, J., Van nigtevecht, G. 1980. Identification, properties and genetic control of UDP-1-rhamnose: anthocyanidin 3-O-glucoside, 6“-O-rhamnosyltransferase from petals of the Red Campion (S. dioica). Z. Naturforsch. 35C:249–257.

    CAS  Google Scholar 

  106. Kamsteeg, J., Van Brederode, J., Van Nigtevecht, G. 1980. The pH-dependent substrate specificity of UDP-Glucose: anthocyanidin 3-rhamnosylglucoside, 5-0-glucosyltransferase in petals of Silene dioica. Z. Pflanzenphysiol 96:87–93.

    CAS  Google Scholar 

  107. Seyffert, W. 1959. Untersuchungen Über interallele Wechselwirkungen III. Der Dosiseffect eines die Methylierung von Anthocyanen kontrollierenden Gene. Naturwissenschaften 46:271.

    Article  Google Scholar 

  108. Harborne, J.B. 1967. Inheritance and biosynthesis of flavonoids in plants. In: Comparative Biochemistry of Flavonoids, Academic Press, London, pp. 250–280.

    Google Scholar 

  109. Gupta, S.B. 1970. Biochemical aspects of the inheritance of floral anthocyanins in diploid alfalfa. Genetics 65:267–278.

    PubMed  CAS  Google Scholar 

  110. Wiering, H., De Vlaming, P. 1977. Glycosylation and methylation patterns of anthocyanins in Petunia hybrida:. The genes Mfl and Mfg. Z. Pfl. Zucht. 78:113–123.

    CAS  Google Scholar 

  111. Jonsson, L.M.V., Aarsman, M.E.G., Schram, A.W., Bennink G.J.H. 1982. Methylation of anthocyanins by cell-free extracts of flower buds of Petunia hybrida. Phytochemistry 21:2457–2459.

    Article  CAS  Google Scholar 

  112. Jonsson, L.M.V., Donker-Koopman, W.E., Uitslager, P., Schram, A.W. 1983. Subcellular localization of anthocyanin methyltransferase in flowers of Petunia hybrida. Plant Physiol. 72:287–290.

    Article  PubMed  CAS  Google Scholar 

  113. Jonsson, L.M.V., De Vlaming, P., Wiering, H., Aarsman, M.E.G., Schram, A.W. 1983. Genetic control of anthocyanin O-methyltransferase activity in flowers of Petunia hybrida.. Theor. Appl. Genet. 66:349–355.

    Article  CAS  Google Scholar 

  114. Jonsson, L.M.V., Aarsman, M.E.G., Poulton, J.E., Schram, A.W. 1984. Properties and genetic control of four methyltransferases involved in methylation of anthocyanins in flowers of Petunia hybrida. Planta 160:174–179.

    Article  CAS  Google Scholar 

  115. Martin, C., Gerats, A.G.M. 1991 The control of flower colouration. In: The Molecular Biology of Flowering. (B. Jordan, ed.) CAB Internat., in press.

    Google Scholar 

  116. Dooner, H.K. 1983. Co-ordinate genetic regulation of flavonoid biosynthetic enzymes in maize. Mol. Gen. Genet. 189:136–141.

    Article  CAS  Google Scholar 

  117. Conn, K.C., Burr, F.A., Burr, B. 1986. Molecular analysis of the maize regulatory locus Cl. Pro. Nat. Acad. Sci. USA 83:9631–9635.

    Article  Google Scholar 

  118. Ludwig, S.R., Habera, L.F., Dellaporta, S.L., Wessler, S.R. 1989. Lc a member of the maize R gene family responsible for tissue specific anthocyanin production encodes a protein similar to transcriptional activators and contains the myc-homology region. Proc. Natl. Acad. Sci. USA 86:849–851.

    Article  Google Scholar 

  119. Goff, S.A., Cone, K.C., Fromm, M.E. 1991. Identification of functional domains in the maize transcriptional activator Cl: comparison of wildtype and dominant inhibitor proteins. Genes and Develop. 5:298–309.

    Article  CAS  Google Scholar 

  120. Rott, B.A., Goff, S.A., Klein, T.M., Fromm, M.E.1991. Cl and R dependent expression of the maize Bzl gene requires sequences with homology to mammalian myb and myc binding sites. Plant Cell 3:317–325.

    Google Scholar 

  121. Goff, S.A., Klein, T.M., Roth, B.A., Fromm, M.E., Cone, K.C., Radicella, J.P., Chandler, V.L. 1990. Transactivation of anthocyanin biosynthetic genes following transfer of of B regulating genes into maize tissues. EMBO J. 9:2517–2522.

    PubMed  CAS  Google Scholar 

  122. Klein, T.M., Roth, B.A., Fromm, M.E. 1989. Regulation of anthocyanin biosynthetic genes introduced into intact maize tissues by microprojectiles. Proc. Natl. Acad. Sci. USA 86:6681–6685.

    Article  PubMed  CAS  Google Scholar 

  123. Paz-Arez, J., Ghosal, D., Wienand, U., Peterson, P.A., Saedler, H. 1987. The regulatory Cl locus of Zea mays encodes a protein with homology to myb protooncogene products and with structural similarities to transcriptional activators. EMBO J. 6:3553–3558.

    Google Scholar 

  124. Chen, S-M. H. 1973. Anthocyanins and their Control by the C locus in Maize. Ph.D. Thesis, Univ. of Missouri, Columbia.

    Google Scholar 

  125. Chen, S-M, Coe, E.H. 1977. Control of anthocyanin synthesis by the C locus in maize. Biochem. Genet. 15:333–346.

    Article  PubMed  CAS  Google Scholar 

  126. Coe, E.H. 1985. Phenotypes in corn: control of pathways by alleles, time and place. In: Plant Genetics. (M. Freeling, ed.) Alan R. Liss Inc., New York, pp 509–521.

    Google Scholar 

  127. Cone, K.C., Burr, B. 1989. Molecular and genetic analyses of light requirement for anthocyanin synthesis in maize. In: The Genetics of Flavonoids. (E.D. Styles, G.A. Gavazzi, M.L. Racchi, eds.) Edizione Unicopli, Milano, pp. 143–146.

    Google Scholar 

  128. Consonni, G., Racchi, M.L., Shammah, S., Gavazzi, G.A. 1987. The role of Sn in the light-regulated activity of enzymes of flavonoid biosynthesis. Maize Genet. Coop. Newsl. 61:35.

    Google Scholar 

  129. Dooner, H.K., Kermicle, J.L. 1971. Structure of the R r tandem duplication in maize. Genetics 67:427–436.

    PubMed  CAS  Google Scholar 

  130. Emerson, R.A. 1921. The genetic relations of plant colours in maize. Cornell Univ. Agric. Exp. Sta. 39:3–156.

    Google Scholar 

  131. Ludwig, S.R., Wessler, S.R. 1990. Maize R gene family: Tissue specific helix-loop-helix proteins. Cell 62:849–851.

    Article  PubMed  CAS  Google Scholar 

  132. Chandler, V.L., Radicella, J.P., Robbins, T.P., Chen, J., Truks, D. 1989. Two regulating genes of the maize anthocyanin pathway are homologous: isolation of B using R genomic sequences. Plant Cell 1:1175–1183.

    PubMed  CAS  Google Scholar 

  133. Stadler, L.D. 1946. Spontaneous mutation at the R locus in maize 1. The aleurone colour and plant colour effects. Genetics 31:377–394.

    Google Scholar 

  134. Coe, E.H., Neuffer, M.G., Hoisington, D.A. 1988. Corn and corn improvement. Agronomy monograph 18:81–258. Amer. Soc. Agron. Inc., Madison, WI USA.

    Google Scholar 

  135. McCarty, J.P., Hattori, T., Vasil, V., Vasil, I.K. 1991. A regulatory hierarchy in seed development:interaction of viviparous-1 and abscisic acid in the regulation of the Cl gene in maize. J. Cell. Biochem., supp. 15A:25.

    Google Scholar 

  136. Taylor, L.P., Briggs, W.R. 1990. Genetic regulation and photocontrol of anthocyanin accumulation in maize seedlings. Plant Cell 2:115–127.

    PubMed  CAS  Google Scholar 

  137. Larson, R.L. 1989. Genetics, precursors and enzymes in flavonoid biosynthesis in maize. In: The Genetics of Flavonoids. (E.D. Styles, G.A. Gavazzi, M.L. Racchi, eds.) Edizione Unicopli, Milano, pp. 71–78.

    Google Scholar 

  138. Gerats, A.G.M., Farcy, E., Wallroth, M., Groot, S.P.C., Schram, A.W. 1984. Control of anthocyanin synthesis in Petunia hybrida by multiple allelic series of the genes An1 and An2. Genetics 106:501–508.

    PubMed  CAS  Google Scholar 

  139. De Vries, H. 1903. Die Mutationstheorie, Band II. Verlag von Veit and Co., Leipzig, pp. 194–206.

    Google Scholar 

  140. Almeida, J., Carpenter, R., Robbins, T.P., Martin, C., Coen, E.S. 1989. Genetic interactions underlying flower colour patterns in Antirrhinum majus. Genes Dev. 3:1758–1767.

    Article  PubMed  CAS  Google Scholar 

  141. Stubbe, H. 1966. Genetik and Zytologie von Antirrhinum L. sec. Antirrhinum. Veb. Gustaf Fischer Verlag, Jena.

    Google Scholar 

  142. Harker, C.L., Ellis, T.H.N., Coen, E.S. 1990. Identification and genetic regulation of the chalcone synthase multigene family in pea. Plant Cell 2:185–194.

    PubMed  CAS  Google Scholar 

  143. Van Der Meer, I.M., Spelt, C.E., Mol, J.N.M., Stuitje, A.R. 1990. Promoter analysis of the chalcone synthase (CHSA) gene of Petunia hybrida:A 67 bp promoter region directs flower-specific expression. Plant Mol. Biol. 15:95–109.

    Article  PubMed  Google Scholar 

  144. Staiger, D., Kaulen, H., Schell, J. 1989. A Cacgtg motif of the Antirrhinum majus chalcone synthase promoter is recognised by an evolutionary conserved nuclear protein. Proc. Natl. Acad. Sci. USA 86:6930–6934.

    Article  PubMed  CAS  Google Scholar 

  145. Lipphardt, S., Brettschneider, R., KREUZALER, F., Schell, J., Dangl, J. 1988. UV-inducible transient expression in parsley protoplasts identifies regulatory cis elements of a chimeric Antirrhinum majus chalcone synthase gene. EMBO J. 7:4027–4033.

    PubMed  CAS  Google Scholar 

  146. Schulze-Lefert, P., Becker-Andre, M., Schulz, W., Hahlbrock, K., Dangl, J. 1989. Functional architecture of light-responsible chalcone synthase promoter from parsley. Plant Cell 1:707–714.

    PubMed  CAS  Google Scholar 

  147. Schulze-Lefert, P., Dangl, J., Becker-Andre, M., Hahlbrock, K., Schulz, W. 1989. Inducible in vivo DNA footprints define sequences necessary for U.V. light activation of the parsley chalcone synthase gene. EMBO J. 8:651–656.

    PubMed  CAS  Google Scholar 

  148. Marocco, A., Wissenbach, M., Becker, D., Paz-Ares, J., Saedler, H., Salamini, F., Rohde, W. 1989. Multiple genes are transcribed in Hordeum vulgare and Zea mays that carry the DNA-binding domain of the Myb oncoprotein. Mol. Gen. Genet. 216:183–187.

    Article  PubMed  CAS  Google Scholar 

  149. Jackson, D., Culianez-Macia, F., Prescott, A., Roberts, K., Martin, C. 1991. Expression patterns of myb genes from Antirrhinum flowers. Plant Cell 3:115–125.

    PubMed  CAS  Google Scholar 

  150. Tabak, A.J.H., Schram, A.W., Bennink, G.J.H. 1981. Modification of the B-ring during flavonoid synthesis in petunia hybrida: effect of the hydroxylation gene Hfl on dihydroflavonol intermediates. Planta 153:462–465.

    Article  CAS  Google Scholar 

  151. Schram, A.W., Timmerman, A.W., DE Vlaming, P., Jonsson, L.M.V., Bennink, G.J.H. 1981. Glucosylation of flavonoids in petals of petunia hybrida. Planta 153:459–461.

    Article  CAS  Google Scholar 

  152. Gerats, A.G.M., Cornelissen, R.T.J., Hogervorst, J.M.W., Schram, A.W., Bianchi, F. 1982. A gene controlling rate of anthocyanin synthesis and mutation frequency of the gene An1 in Petunia hybrida. Theor. Appl. Gen. 62:199–203.

    CAS  Google Scholar 

  153. De Vlaming, P., Schram, A.W., Wiering, H. 1983. Genes affecting flower colour and pH of flower limb homogenates in Petunia hybrida. Theor. Appl. Genet. 66:271–278.

    Article  Google Scholar 

  154. Scott-Moncrieff, R.A. 1936. A biochemical survey of some mendelian factors for flower colour. J. Genet. 32:117–170.

    Article  Google Scholar 

  155. Harborne, J.B. 1967. The anthocyanin pigments. In: Comparative Biochemistry of Flavonoids, Academic Press, London, pp. 1–30.

    Google Scholar 

  156. Cornu, A., Farcy, E., Maizonnier, D., Haring, M., Veerman, W., Gerats, A.G.M. 1990. Petunia hybrida (2n=14). In: Genetic Maps: Locus Maps of Complex Genomes. Book 6, Plants. (S.J. O’Brien, ed.) Cold Spring Harbor Lab. Press, fifth ed., pp. 6.113–6.124.

    Google Scholar 

  157. De Vlaming, P., Van Eekeres, J.E.M., Wiering, H. 1982. A gene for flower colour fading in Petunia hybrida. Theor. Appl. Genet. 61:41–46.

    Article  Google Scholar 

  158. Gerats, A.G.M., Bussard, J., Coe, E.H., Larson, R. 1984. Influence of B and Pl on UDPG flavonoid 3-O-glucosyltransferase in Zea mays L. Biochem. Genet. 22:1161–1169.

    Article  PubMed  CAS  Google Scholar 

  159. Gavazzi, G., Mikerezi, I., Papinutti, P., Tonelli, C. 1985. Light induced effects on tissue specific gene expression in Zea mays L. Maydica 30:309–319.

    CAS  Google Scholar 

  160. Schroeder, G., Brown, J.W.S., Schroeder, J. 1988. Molecular analysis of resveratrol synthase. cDNA, genomic clones and relationship with chalcone synthase. Eur. J. Biochem. 172:161–169.

    Article  CAS  Google Scholar 

  161. Wagner, G.J. 1979. Content and vacuole/extravacuole distribution of neutral sugars, free amino acids and anthocyanin in protoplasts. Plant Physiol. 64:88–93.

    Article  PubMed  CAS  Google Scholar 

  162. Wiermann, R., Buth-Weber, M. 1980. The distribution and localization of an UDP-Glucose:flavonol 3-O-glucosyl transferase activity in pollen. Protoplasma 104:307–313.

    Article  CAS  Google Scholar 

  163. Wiermann, R., Vieth, K. 1983. Outer pollen wall, an important accumulation site for flavonoids. Protoplasma. 118:230–233.

    Article  CAS  Google Scholar 

  164. Robertson, D.S. 1965. A dormant allele of vpl. Maize Gen. Coop. Newsl. 39:104.

    Google Scholar 

  165. Robichaud, C.S., Sussex, I.M. 1986. The response of viviparous-1 and wildtype embryos of Zea mays to culture in the presence of abscisic acid. J. Plant Physiol. 126:235–242.

    Article  CAS  Google Scholar 

  166. Guruprasad, K.N., Laloraya, M.M. 1980. Effect of pigment precursors on the inhibition of anthocyanin biosynthesis by GA and ABA. Plant Sci. Lett. 19:73–79.

    Article  CAS  Google Scholar 

  167. Hinderer, W., Petersen, M., Seitz, H.U. 1984. Inhibition of flavonoid biosynthesis by gibberellic acid in cell suspension cultures of Daucus carota L. Planta 160:544–549.

    Article  CAS  Google Scholar 

  168. Barendse, G.W.M., Pereira, R.A.S., Barkers, P.A., Driessen, F.M., Van Eyden, E.A., Linskens H.F. 1970. Growth hormones in pollen, styles and ovaries of Petunia hybrida and Lilium species. Acta Bot. Neerl. 19:175–186.

    CAS  Google Scholar 

  169. Weiss, D., Halevy, A.H. 1989. Stamens and gibberellin in the regulation of corolla pigmentation and growth in Petunia hybrida. Planta 179:89–96.

    Article  CAS  Google Scholar 

  170. Weiss, D., Van Tunen, A.J., Halevy, A.H., Mol, J.N.M., Gerats, A.G.M. 1990. Stamens and gibberellic acid in the regulation of flavonoid gene expression in the corolla of Petunia hybrida. Plant Physiol. 94:511–515.

    Article  PubMed  CAS  Google Scholar 

  171. Van Der Krol, A.R., Lenting, P.J., Veenstra, J.G., VAN Der Meer, I.M., Koes, R.E., Gerats, A.G.M., Mol, J.N.M., Stuitje, A.R. 1988. An antisense chalcone synthase gene in transgenic plants inhibits flower pigmentation. Nature 333:866–869.

    Article  Google Scholar 

  172. Van Der Krol, A.R., Mur, L.A., De Lange, P., Gerats, A.G.M., Mol, J.N.M., Stuitje, A.R. 1990. Antisense chalcone synthase genes in Petunia: visualisation of variable transgene expression. Mol. Gen. Genet. 220:204–212.

    Article  Google Scholar 

  173. Van Der Krol, A.R., Mur, L.A., Beld, M., Mol, J.N.M., Stuitje, A.R. 1990. Flavonoid genes in Petunia: addition of a limited number of gene copies may lead to a suppression of gene expression. Plant Cell 2:291–299.

    PubMed  Google Scholar 

  174. Van Der Krol, A.R., Mur, L.A., Mol, J.N.M., Stuitje, A.R. 1990. Inhibition of flower pigmentation by antisense CHS genes: promoter and minimal sequence requirements for the antisense effects. Plant Mol. Biol. 14:457–466.

    Article  PubMed  Google Scholar 

  175. Napoli, C., Lemieux, C., Jorgensen, R. 1990. Introduction of a chimeric chalcone synthase gene into Petunia: results in reversible co-suppression of homologous genes in trans. Plant Cell 2:279–289.

    PubMed  CAS  Google Scholar 

  176. Jorgensen, R. 1990. Altered gene expression in plants due to trans interactions between homologous genes. TlBtech. 8:340–344.

    Article  CAS  Google Scholar 

  177. Brakenhoff, G.J., Van Der Voort, H.T.M., Oud, J.L. 1990 Three dimensional image representation in confocal microscopy. In: Confocal Microscopy. (T. Wilson, ed.) Acad. Press, London, pp. 185–197.

    Google Scholar 

  178. Nanninga, N., Oud, J.L. 1990. Analysis of chromosomes by CSLM. Eur. Microscopy and Analysis, pp. 23–25.

    Google Scholar 

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Gerats, A.G.M., Martin, C. (1992). Flavonoid Synthesis in Petunia Hybrida; Genetics and Molecular Biology of Flower Colour. In: Stafford, H.A., Ibrahim, R.K. (eds) Phenolic Metabolism in Plants. Recent Advances in Phytochemistry, vol 26. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3430-3_6

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