Distribution Pattern of Capillary and Venular Red Blood Cell Velocity Following Ischemia-Reperfusion in Striated Muscle*
Microvascular red blood cell (RBC) velocity in striated muscle fluctuates with time and varies from capillary to capillary (Ellis et al., 1990). The heterogeneity of microvascular RBC-velocity is known to be enhanced in pathologic conditions, such as ischemia-reperfusion (Tyml and Budreau, 1988; Menger et al., 1988). Previous studies have shown that microvascular ischemia-reperfusion injury is located primarily in nutritive capillaries and postcapillary venules (Menger et al., 1989; Lehr et al., 1991; Granger et al., 1989). The aim of the present study was to analyze the distribution pattern of capillary and venular RBC-velocity following ischemia-reperfusion to striated muscle. Using the hamster dorsal skinfold preparation intravital fluorescence microscopy provides a direct access to the striated muscle microcirculation, allowing repeated observations over a prolonged period of time in the awake animal, including quantitative analysis of different microhemodynamic parameters (Endrich et al., 1980).
KeywordsSyrian Golden Hamster Postcapillary Venule Awake Animal Postischemic Reperfusion Intravital Fluorescence Microscopy
Unable to display preview. Download preview PDF.
- Ellis, C.G., Wrigley, S.M., Potter, R.F., and Groom, A.C., 1990, Temporal distributions of red cell supply rate to individual capillaries of resting skeletal muscle, in frog and rat, Int. J. Microcir.: Clin. Exp., 9: 67.Google Scholar
- Lewis, D.H., 1984, The response of the microvasculature in skeletal muscle to hemorrhage, trauma; and ischemia, Prog. Appl. Microcirc, 5: 127.Google Scholar
- Menger, M.D., Hammersen, F., Barker, J., Feifel, G., and Messmer, K., 1989, Ischemia and reperfusion in skeletal muscle: Experiments with tourniquet-ischemia in the awake Syrian golden hamster. Prog. Appl. Microcirc, 13: 93.Google Scholar
- Tyml, K., and Budreau, C.H., 1988, Heterogeneity of microvascular response to ischemia in skeletal muscle, Int. J. Microcirc: Clin. Exp., 7: 205.Google Scholar