Microtopographic Analysis of Oxidative Stress in Organ Microcirculatory Units

  • Makoto Suematsu
  • Masaharu Tsuchiya
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 316)


In eukaryotic cells, molecular oxygen functions primarily as the terminal acceptor of electrons during mitochondrial electron transport. Most of oxygen consumed in this process is reduced to water by cytochrome oxidase. Only a small percentage of the total O2 consumption appears as partially reduced species such as O2- and H2O2. It is likely, however, that under pathological situations, these active oxidants may be highly generated and can exert, in turn, their cytotoxic properties. It is technically difficult to obtain spatial and temporal information of intravital oxidative stress. Since most observations which suggested participation of active oxidants or lipid peroxides in tissue injury are based upon preventive effects of antioxidants, establishment of the microscopic oxidant-visualizing system has been awaited to further clarify microtopographic relationship between oxyradical generation and tissue breakdown. This report is therefore aimed to summarize the methodology for oxyradical visualization in vivo.


Xanthine Oxidase Chronic Granulomatous Disease Tissue Breakdown Renal Epithelial Cell Line Active Oxygen Metabolite 
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Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Makoto Suematsu
    • 1
  • Masaharu Tsuchiya
    • 1
  1. 1.Department of Internal Medicine, School of MedicineKeio UniversityTokyoJapan

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