Advertisement

The Simultaneous Measurement of the Redox State of Cytochrome Oxidase in Heart and Brain of Rat in Vivo by NIR

  • Yasuyuki Kakihana
  • Kinji Ito
  • Mamoru Tamura
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 316)

Abstract

Cytochrome oxidase(Cyt.ox.), which contains heme and copper, is a terminal enzyme of respiratory chain and transfers electrons to molecular oxygen (Erecinska & Wilson 1978). For this reason, monitoring oxidation-reduction state of Cyt.ox. provides the information about the pathologic state of tissues caused by impaired oxygen supply. The Cyt.ox., hemoglobin(Hb) and myoglobin(Mb) have a broad absorption band in near infrared(NIR) region and the light in this region easily penetrates biological tissues. Thus NIR spectrophotometry can measure noninvasively the redox state of Cyt.ox. and oxygenation of Hb and Mb through skin and bone. In this paper, to obtain the mutual relationship between the oxygenation state of cardiac and brain tissues, we examined the redox behavior of copper in Cyt.ox. with anesthetized rat.

Keywords

Redox State Cytochrome Oxidase Oxygenation State Carbon Dioxide Concentration Light Guide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Erecinska, M., Wilson, D.F. (1978) “Cytochrome C oxidase; A synopsis.” Arch.Biochem.Biophys. 188:1PubMedCrossRefGoogle Scholar
  2. Hazeki, O., and Tamura, M., (1989) “Near infrared quadruple wavelength spectrophotometry of rat head” Adv.Exp.Med.Biol. 248:63PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Yasuyuki Kakihana
    • 1
  • Kinji Ito
    • 1
  • Mamoru Tamura
    • 1
  1. 1.Biophysics Division, Research Institute of Applied ElectricityHokkaido UniversitySapporoJapan

Personalised recommendations