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Intravitreal and Intraretinal Oxygen Tension in the Rat Eye

  • Stephen J. Cringle
  • Dao-Yi Yu
  • Valerie A. Alder
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 316)

Abstract

The intravitreal and intraretinal oxygen distribution has been studied in several larger animals1,2,3 but never before in the rat. In fact the small vitreal volume (55µl)4 and large lens has previously dissuaded attempts at intraocular microelectrode work of any description. However, the advent of several rat models of retinal vascular disease has made this animal an attractive model in which to study retinal oxygenation, with a view to a better understanding of human diseases of a vascular nature. We therefore modified our techniques developed for larger animals to allow comparable measurements to be made in the rat. Vitreal oxygen tension distribution has been determined, with particular attention to the effects of retinal arteries and veins. The intraretinal oxygen tension profIle has also been categorised. The effects of hyperoxic ventilation were also investigated intravitreally and intraretinally. This control data will be vital to our future studies of the effects of vascular impairment in models of vascular disease.

Keywords

Oxygen Tension Retinal Thickness Internal Limit Membrane Retinal Location Oxygen Profile 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    V.A. Alder, S.J. Cringle, and I.J. Constable, The retinal oxygen profile in cat. Invest. Ophthalmol. Vis. Sci. 24:30–36 (1983).PubMedGoogle Scholar
  2. 2.
    R.A. Linsenmeier, and C.M. Yancey, Effects of hyperoxia on the oxygen distribution in the intact cat retina. Invest. Ophthalmol. Vis. Sci. 30, 612 (1989).PubMedGoogle Scholar
  3. 3.
    M. Tsacopoulos, R. Baker, and S. Levy, Studies on retinal oxygenation. Adv Exp Med Biol 75:413 (1976).PubMedGoogle Scholar
  4. 4.
    A. Chaudhurl, P.E. Hallett, and J.A. Parker, Aspheric curvatures, refractive indices and chromatic aberration for the rat eye. Vis. Res. 23:1351 (1983).CrossRefGoogle Scholar
  5. 5.
    R.A. Linsenmeier, Effects of light and darkness on oxygen consumption and distribution in the cat retina. J Gen Physiol 88:521 (1986).PubMedCrossRefGoogle Scholar
  6. 6.
    V.A. Alder, and S.J. Cringle, Intraretinal and preretinal PO2 response to acutely raised intraocular pressure in cats. Am J Physiol. 256 (Heart. Circ. Physiol. 25):H1627 (1989).PubMedGoogle Scholar
  7. 7.
    V.A. Alder and S.J. Cringle, The effect of the retinal circulation on vitreal oxygen tension. Curr. Eye Res. 4:121 (1985).PubMedCrossRefGoogle Scholar
  8. 8.
    C.J. Pournaras, C.E. Riva, M. Tsacopoulos, and K. Strommer, Diffusion of O2 in the retina of anaesthetized miniature pigs in nonnoxia and hyperoxia. Exp. Eye Res. 49:347(1989).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Stephen J. Cringle
    • 1
  • Dao-Yi Yu
    • 1
  • Valerie A. Alder
    • 1
  1. 1.Lions Eye Institute and Department of SurgeryUniversity of Western Australia NedlandsWestern AustraliaAustralia

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