Abstract
A silver colloid technique for the staining of nucleolar organizer regions (NORs) was applied to paraffin sections of 52 clinical prostate cancers, 5 incidental carcinomas of the prostate, 12 benign prostatic hypertrophy (BPH) specimens and 7 normal prostates. The mean numbers of silver-stained NORs (AgNORs) in these lesions were 3.12±0.52 in clinical cancer, 2.65±0.64 in incidental cancer, 1.66±0.16 in BPH, and 1.76±0.22 in normal prostate. There was a statistically significant difference in agNORs numbers between cancer and benign prostatic tissues (p<0.001). However, no significant difference was observed in AgNORs numbers between incidental and clinical carcinoma of the prostate. In clinical cancer, only poorly differentiated adenocarcinoma showed a statistically larger number of AgNORs than the well or moderately differentiated group (p<0.02). Correlation between AgNORs numbers and clinical stage was not obvious. There was no relationship between the number of AgNORs and serum values of tumor markers such as PAP, PSA and γ - Sm. Moreover, the AgNORs numbers did not show a relation to decreasing rates of serum marker levels during successful anti-androgen therapy. If the patients with prostate cancer were divided into two groups by 2.9 of AgNORs number, the group with the smaller number of AgNORs (n=14) was found to have a tendency towards a longer disease-stabilizing period than the larger group (n=17).
Keywords
- Prostate Cancer
- Nucleolar Organizer Region
- Normal Prostate
- Benign Prostatic Hypertrophy
- Embryonal Rhabdomyosarcoma
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References
Howell E. and Black D.A., Controlled silver staining of nucleolar organizing regions with protective colloidal developer: a one-step method, Experientia 36:1014 (1980).
Ploton D., Bobinchon H. and Adnet J.J., Ultrastructual localization of NOR in nucleoli of human breast cancer tissues using a one-step AgNOR staining method, Biol. Cell. 43:229–232 (1982).
Ploton D., Menager M. Heannesson P., Himber G., Pigeon F. and Adent J.J., Improvement in the staining and visualization of the argyrophillic proteins of the nucleolar organizer region at the optical level, Histochem. J. 18:5–14 (1986).
Crocker J. and Nar P. Nucleolar organizing regions in lymphomas, J. Pathol. 141:111–118 (1987).
Takeuchi T., Tanaka T., Ohno T., Yamamoto N., Kobayashi S., Kuriyama M., Kawada Y. and Mori H., Nucleolar organizer regions in rat urinary bladder tumos induced by N-butyl-N-(4-hydroxybutyl) nitrosamine, Virchom. Arch. 58:383–387 (1990).
Takeuchi T., Study on nucleolar organizer regions in bladder neoplasm, Jpn. J. Urol. 81:1711–1719 (1990).
Yamamoto N., Takeuchi T., Kuriyama M., Tanaka T. and Kawada Y., Nucleolar organizer regions (AgNORs) in renal cell carcinoma, 15th International Cancer Congress, Hamburg, FRG, August (1990).
Hernandes-Verdun D., Derenzini M. and Bouteille M. Relationship between AgNOR proteins and ribosomal chromatin in situ during drug induced inhibition, J. Ultrastruc. Res. 88:55–65 (1984).
Das B.S., Rani R., Mitra A.B. and Luthra U.K. The number of silver staining NOR’s (rDNA) in lymphocytes of newborns and its relationship to human development, Mech. Aging. Dev. 36:117–123 (1986).
Guillaud P., du Manoir S. and Seigneurin D., Quantitation and topographical distribution of Ki67 antibody labelling during the cell cycle of normal fibroblastic (MRC-5) and mammary tumor cell lines (MCF-7), Analyt. Cell. Pathol. 1:25–39 (1989).
Jan-Mohamed R.M., Armstrong S.L., Crocker J., Leyland M.J. and Hulten M.A., The relationship between numbers of interphase NORs and NOR-bearing chromosomes in non-Hodgkin’s lymphoma, J. Pathol. 158:3–7 (1989).
Derenzini M., Pession A., Farabegoli F., Badiali M. and Dehan P., Relationship between interphasic nucleolar organizer regions and growth rate in two neuroblastoma cell lines, Am. J. Pathol. 134:925–932 (1989).
Crocker J. and Skilbeck N., Nucleolar organizer region-associated protein in melanotic lesions of the skin. A quantitative study, J. Clin. Pathol. 49:885–889 (1987).
Cohen R.J., Glezerson G., Haffejee Z. and Afrika D., Prostatic carcinoma: Histological and immunohistological factors affecting prognosis, Brit. J. Urol. 66:405–410 (1990).
Ruschoff J., Bittinger A., Neumann K. and Schmitz-Moormann P., Prognostic significance of nucleolar organizing regions (NORs) in carcinomas of the sigmoid colon and rectum, Path. Res. Pract. 186:85–91 (1990).
Egan M. J., Raafat F., Crocker J. and Williams D., Comparative study of the degree of differentiation of neuroblastoma and mean numbers of nucleolar organizer regions, J. Clin. Pathol. 41:527–531 (1988).
Egan M.J., Raafat F., Crocker J. and Williams D., Prognostic importance of nucleolar organizer regions in embryonal rhabdomyosarcoma, J. Pathol. 154:477 (1988).
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© 1992 Springer Science+Business Media New York
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Kobayashi, S. et al. (1992). Nucleolar Organizer Regions in Prostate Cancer. In: Karr, J.P., Yamanaka, H. (eds) Prostate Cancer and Bone Metastasis. Advances in Experimental Medicine and Biology, vol 324. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3398-6_19
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DOI: https://doi.org/10.1007/978-1-4615-3398-6_19
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