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Determination of Boron Concentration in Tissues by Means of Alpha Autoradiography

  • Jeffrey E. Woollard
  • Yirun Jiang
  • James F. Curran
  • Thomas E. Blue
  • Rolf F. Barth

Abstract

The accurate measurement of total boron content in biological samples with a sensitivity in the ppm range is essential for evaluating the potential usefulness of various tumor localizing boron-containing compounds for Boron Neutron Capture Therapy (BNCT)1. A group of analytical procedures for boron quantitation are those involving nuclear methods. These include the detection of alpha particles resulting from the 10B(n,α,γ)7Li reaction by means of alpha track autoradiography2,3 and the measurement of gamma photons, by means of prompt gamma analysis4. The purpose of the present study was to develop alpha track autoradiography for microdistribution studies. It utilizes a solid state nuclear track detector (SSNTD), CR-39, which is composed of a polycarbonate resin, to record the charged products of the 10B(n, α)7Li reaction.

Keywords

Boron Concentration Boron Neutron Capture Therapy Track Density Solid State Nuclear Track Detector Common Coordinate System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    R.F. Barth, A.H. Soloway, R.G. Fairchild: Boron Neutron Capture Therapy of Cancer, Cancer Res. 50, 1061–1070 (1990).PubMedGoogle Scholar
  2. 2.
    M. Abe, K. Amano, K. Kitamura, J. Tateishi, H. Hatanaka: Boron Distribution Analysis by Alpha-Autoradiography, J. Nucl. Med. 27, 677–684 (1986).PubMedGoogle Scholar
  3. 3.
    D. Gabel, H. Holstein, B. Larsson, L. Gille, G. Erikson, D. Sacker, P. Som and R. G. Fairchild: Quantitative Neutron Capture Radiography for Studying the Biodistribution of Tumor-seeking Boron-containing Compounds, Cancer Res. 47, 5451–5456 (1987).PubMedGoogle Scholar
  4. 4.
    R.G. Fairchild, D. Gabel, B.H. Laster, D. Greenberg, W. Kiszenick, P.F. Micca: Microanalytical Techniques for Boron Analysis Using the 10B(n, α)7Li reaction, Med. Phys. 13, 50–56 (1986).PubMedCrossRefGoogle Scholar
  5. 5.
    N.R. Clendenon, R.F. Barth, W.A. Gordon, J.H. Goodman, F. Alam, A.E. Staubus, C.P. Boesel, A.J. Yates, M.L. Moeschberger, R.G. Fairchild, J.A. Kalef-Ezra: Boron Neutron Capture Therapy of a Rat Glioma, Neurosurgery 26, 47–55 (1990).PubMedCrossRefGoogle Scholar
  6. 6.
    J.E. Woollard, T.E. Blue, J.F. Curran, T.F. Mengers, R.F. Barth: An Alpha Autoradiographic Technique for Determination of 10B Concentrations in Blood and Tissue. Nucl. Instr. and Meth. A 299, 600–605 (1990).CrossRefGoogle Scholar
  7. 7.
    J.E. Woollard,T.E. Blue, J.F. Curran, M. C. Dobelbower, H. R. Busby: An Alpha Autoradiographic Technique for Spatial Quantification of Boron-10 Concentrations in Tissue, Nucl. Sci.and Eng.(in press).Google Scholar
  8. 8.
    M. Bendayan, R.F. Barth, D. Gingras, I. Londono, P.T. Robinson, F. Alam, D.M. Adams, L. Mattiazzi: Electron Spectroscopic Imaging for High Resolution Immunocytochemistry: Use of Boronated Protein A, J. Histochem Cytochem 37, 573–580 (1989).PubMedCrossRefGoogle Scholar
  9. 9.
    W.A. Ausserer, Y.C. Ling, S. Chandra, G.H. Morrison: Quantitative Imaging of Boron, Calcium, Magnesium, Potassium, and Sodium Distributions in Cultured Cells With Ion Microscopy, Anal. Chem. 61, 2690–2695 (1989).PubMedCrossRefGoogle Scholar
  10. 10.
    R.F. Barth, D.M. Adams, A.H. Soloway, E.B. Mechetner, F. Alam and A.K.M. Anisuzzaman: Determination of Boron in Tissues and Cells Using Direct-Current Plasma Atomic Emission Spectroscopy, Anal. Chem. 63, 4 (1991).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Jeffrey E. Woollard
    • 1
  • Yirun Jiang
    • 1
  • James F. Curran
    • 1
  • Thomas E. Blue
    • 1
  • Rolf F. Barth
    • 2
  1. 1.Nuclear Engineering ProgramThe Ohio State UniversityColumbusUSA
  2. 2.Department of PathologyThe Ohio State UniversityColumbusUSA

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