Summary
Intermittent-light grown plants were exposed to continuous illumination, and after various periods of time in the light, thylakoids were isolated from the leaves. A two-dimensional PAGE system was used to examine changes in the protein content of the photosynthetic apparatus and of the individual multiprotein complexes of photosystems I and II in the developing plastids.
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1.
The light-harvesting complex of photosystem I (LHC I) was added to the P700-containing core complex (CC I) to yield a complete photosystem I unit by 24 hours in continuous light. Its addition correlated with (a) increases in the size and number of 11 — 24kDa polypeptides in the photosystem I component, and (b) a shift to longer wavelengths of the 77°K fluorescence peak.
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2.
Synthesis of the major LHC II pigment-protein (LHC lib) occurs via addition of pigments to the monomeric polypeptide prior to assembly of the pigmented monomer into the trimer.
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3.
Many polypeptides of unknown function, some of which are constituents of large multiprotein complexes, disappear as the protochloroplast membranes develop into chloroplast thylakoids.
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Jaing, J.T., Welty, B.A., Morishige, D.T., Thornber, J.P. (1992). Assembly of the Photosystem I Multiprotein Complex and the Oligomeric Form of the Major Light-Harvesting Chlorophyll a/b-Protein in Pea Seedlings Grown in Flashed Light Followed by Continuous Illumination. In: Argyroudi-Akoyunoglou, J.H. (eds) Regulation of Chloroplast Biogenesis. Nato ASI Series, vol 226. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3366-5_42
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