Effect of Phosphonic Acid Esters on Photoconversion of Protochlorophyllide in Barley Etioplasts

  • Fabrice Franck
  • Olga Górnicka
  • Kazimierz Strzałka
Part of the Nato ASI Series book series (NSSA, volume 226)


Photoactive protochlorophyllide (pchlide) exists in etioplasts in the form of an active complex with the enzyme and NADPH, which has 77 K fluorescence maximum at 657 nm. In this complex pchlide is reduced into chlorophyllide (chlide) as soon as light is turned on. Part of the pchlide pool cannot be photoconverted because either the pigment or NADPH is not bound to the enzyme. This inactive pchlide has 77 K fluorescence maximum at 633 nm. After the initial photoconversion of the active complex, some inactive pchlide is transformed into new photoactive one [Oliver and Griffiths, 1982]. The photoactive pchlide is located mainly in the prolamellar body [Lindsten et al., 1988], where it is thought to form aggregates [Böddi et al., 1989]. The relationship between the ordered structure of the prolamellar body and the occurrence of the photoactive pchlide has not yet been established, although some data suggest that such relationship may exist [Klein and Schiff, 1972; Grevby et al., 1989].


Phosphonic Acid Polypeptide Composition Prolamellar Body Etioplast Membrane Isolate Thylakoid Membrane 
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Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Fabrice Franck
    • 1
  • Olga Górnicka
    • 2
  • Kazimierz Strzałka
    • 2
  1. 1.Lab. Photobiologie, B22Liege UniversityLiegeBelgium
  2. 2.Inst. Molecular BiologyJagiellonian UniversityKrakówPoland

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