Summary
We conclude from these results that transport of the 23K and 33K across the thylakiod membrane requires a protonmotive force. The dominant component, in terms of driving protein transport, is the proton gradient and not the electrical potential. Interestingly, Theg et al. (1989) have reported that the transport of the small electron carrier protein plastocyanin, does not require a pmf for efficient import into the thylakoid lumen. We have used pre-plastocyanin as an import substrate in our import assays and our results are in agreement. It remains to be determined exactly how a pmf drives transport of the 23K and 33K across the thylakoid membrane.
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References
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© 1992 Springer Science+Business Media New York
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Mould, R.M., Robinson, C. (1992). Protein Targeting to the Thylakoid Lumen. In: Argyroudi-Akoyunoglou, J.H. (eds) Regulation of Chloroplast Biogenesis. Nato ASI Series, vol 226. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3366-5_22
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DOI: https://doi.org/10.1007/978-1-4615-3366-5_22
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