Excitation-Contraction Coupling in Rat Skeletal Muscle Cells: Evolution During in Vitro Myogenesis
The fusion of mononucleated myoblasts into multinucleated myotubes is a crucial event during myogenesis since it precedes (without being a prerequisite) the appearance of a lot of molecules and processes vital for the cell: T system formation and sarcotubular organization, contractile proteins arrangements, voltage-dependent calcium channels, sarcoplasmic reticulum Ca-ATPase, junctional nicotinic receptor (see reviews from Schneider and Olson, 1988; Nathanson, 1989). Therefore the study of events taking place before and after fusion will be of great importance for the understanding of mechanisms controlling the main function of muscle cells, that is contraction.
KeywordsCalcium Signal Calcium Current Calcium Transient Repetitive Pulse Caffeine Contracture
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