Purification of a Ryanodine-Sensitive Channel Protein from Human Neutrophils

  • David P. Rardon
  • Philip C. Krause
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 311)


In cardiac and skeletal muscle excitation-contraction coupling is dependent upon release of calcium (Ca2+) from junctional sarcoplasmic reticulum via a ryanodine-sensitive Ca2+-release channel. Similar oscillatory changes in intracellular Ca2+ concentration are important for the normal function of neutrophils. Therefore, we postulated the existence of a ryanodine-sensitive Ca2+ channel in human neutrophils. This hypothesis is supported by our prior observation that ryanodine (10 nM) increased the intracellular Ca2+ content of intact human neutrophils1.


Channel Activity Human Neutrophil Release Channel Slope Conductance Size Exclusion Column 
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  1. 1.
    D.P. Rardon, and L.P. Akard, Ryanodine regulation of intracellular calcium and divalent cation channel activity in human neutrophils, Circulation, 80:II–519, (1989).Google Scholar
  2. 2.
    T.G. Gabig, D. English, L.P. Akard, and M.J. Schell, Regulation of neutrophil NADPH oxidase activation in a cell-free system by guanine nucleotides and fluoride, J. Biol. Chem., 262:1685–1690, (1987).PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • David P. Rardon
    • 1
  • Philip C. Krause
    • 1
  1. 1.Krannert Institute of CardiologyIndiana University School of MedicineIndianapolisUSA

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