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Purification of a Ryanodine-Sensitive Channel Protein from Human Neutrophils

  • David P. Rardon
  • Philip C. Krause
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 311)

Abstract

In cardiac and skeletal muscle excitation-contraction coupling is dependent upon release of calcium (Ca2+) from junctional sarcoplasmic reticulum via a ryanodine-sensitive Ca2+-release channel. Similar oscillatory changes in intracellular Ca2+ concentration are important for the normal function of neutrophils. Therefore, we postulated the existence of a ryanodine-sensitive Ca2+ channel in human neutrophils. This hypothesis is supported by our prior observation that ryanodine (10 nM) increased the intracellular Ca2+ content of intact human neutrophils1.

Keywords

Channel Activity Human Neutrophil Release Channel Slope Conductance Size Exclusion Column 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    D.P. Rardon, and L.P. Akard, Ryanodine regulation of intracellular calcium and divalent cation channel activity in human neutrophils, Circulation, 80:II–519, (1989).Google Scholar
  2. 2.
    T.G. Gabig, D. English, L.P. Akard, and M.J. Schell, Regulation of neutrophil NADPH oxidase activation in a cell-free system by guanine nucleotides and fluoride, J. Biol. Chem., 262:1685–1690, (1987).PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • David P. Rardon
    • 1
  • Philip C. Krause
    • 1
  1. 1.Krannert Institute of CardiologyIndiana University School of MedicineIndianapolisUSA

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