Purification of a Ryanodine-Sensitive Channel Protein from Human Neutrophils
In cardiac and skeletal muscle excitation-contraction coupling is dependent upon release of calcium (Ca2+) from junctional sarcoplasmic reticulum via a ryanodine-sensitive Ca2+-release channel. Similar oscillatory changes in intracellular Ca2+ concentration are important for the normal function of neutrophils. Therefore, we postulated the existence of a ryanodine-sensitive Ca2+ channel in human neutrophils. This hypothesis is supported by our prior observation that ryanodine (10 nM) increased the intracellular Ca2+ content of intact human neutrophils1.
KeywordsSucrose Fluoride CaCl NADPH HEPES
- 1.D.P. Rardon, and L.P. Akard, Ryanodine regulation of intracellular calcium and divalent cation channel activity in human neutrophils, Circulation, 80:II–519, (1989).Google Scholar