Summary
The effects of inorganic phosphate (Pi) on the sarcoplasmic reticulum (s.r.) were studied in saponin-treated rat cardiac muscle. Ca2+-uptake and release by the s.r. was detected using Indo-1. Oscillations of Ca2+ and tension occurred when the [Ca2+] of the bathing solution was around the threshold for force production. Introduction of Pi (20mM) caused a slow transient release of Ca2+ from the s.r. The oscillations were abolished in the presence of Pi. Removal of Pi caused a rapid fall in Ca2+ followed by a large transient increase in [Ca2+] and tension. Results are consistent with a phosphate-induced decrease Ca2+ available for release.
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References
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KAMMERMEIER, H., SCHMIDT, P. & JUNGLING, E. (1982). Journal of Molecular and Cellular Cardiology 14, 267–277.
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© 1992 Springer Science+Business Media New York
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Smith, G.L., Steele, D.S., Crichton, C.A. (1992). Effects of Inorganic Phosphate on Calcium and Tension Oscillations in Saponin-Treated Rat Cardiac Muscle. In: Frank, G.B., Bianchi, C.P., ter Keurs, H.E.D.J. (eds) Excitation-Contraction Coupling in Skeletal, Cardiac, and Smooth Muscle. Advances in Experimental Medicine and Biology, vol 311. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3362-7_47
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DOI: https://doi.org/10.1007/978-1-4615-3362-7_47
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6483-2
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