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Effects of Inorganic Phosphate on Calcium and Tension Oscillations in Saponin-Treated Rat Cardiac Muscle

  • G. L. Smith
  • D. S. Steele
  • C. A. Crichton
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 311)

Summary

The effects of inorganic phosphate (Pi) on the sarcoplasmic reticulum (s.r.) were studied in saponin-treated rat cardiac muscle. Ca2+-uptake and release by the s.r. was detected using Indo-1. Oscillations of Ca2+ and tension occurred when the [Ca2+] of the bathing solution was around the threshold for force production. Introduction of Pi (20mM) caused a slow transient release of Ca2+ from the s.r. The oscillations were abolished in the presence of Pi. Removal of Pi caused a rapid fall in Ca2+ followed by a large transient increase in [Ca2+] and tension. Results are consistent with a phosphate-induced decrease Ca2+ available for release.

Keywords

Free Energy Smooth Muscle Myocardial Ischaemia Control Level Sarcoplasmic Reticulum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. KENTISH, J.C. (1985). Journal of Physiology 370, 585–604.Google Scholar
  2. KAMMERMEIER, H., SCHMIDT, P. & JUNGLING, E. (1982). Journal of Molecular and Cellular Cardiology 14, 267–277.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • G. L. Smith
    • 1
  • D. S. Steele
    • 1
  • C. A. Crichton
    • 1
  1. 1.Institute of PhysiologyUniversity of GlasgowGlasgowScotland

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