Calcium and Potassium Channel Regulation by Protein Phosphorylation in Smooth Muscle Cells of Guinea Pig Taenia Coli

  • Hideyo Yabu
  • Kazuo Obara
  • Toshihiro Usuki
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 311)


Generally speaking, Ca2+ influx through membrane in smooth muscle cells may play a crucial role in Excitation-Contraction Coupling. Mammalian smooth muscle cells are universally endowed with a lot of L-type, dihydropyridine-sensitive voltage-dependent Ca2+ channels and various kinds of K+ channels. Actually, Ca2+ conductances are increased by membrane depolarization causing Ca2+ influx and subsequent contraction.


Smooth Muscle Cell Phorbol Ester Okadaic Acid Outward Current Inositol Trisphosphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Benham, C.D., Bolton, T.B. and Lang, R.J., 1985, Acetylcholine activates an inward current in single mammalian smooth muscle cells, Nature, 316: 345–347.PubMedCrossRefGoogle Scholar
  2. Hamill, O.P., Marty, A., Neher, E., Sakmann, B. and Sigworth, F.J., 1981, Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflügers Arch., 391: 85–100.PubMedCrossRefGoogle Scholar
  3. Hescheler, J., Mieskes, G., Rüegg, J.C., Takai, A. and Trautwein, W., 1988, Effects of a protein phosphatase inhibitor, okadaic acid, on membrane currents of isolated guinea-pig cardiac myocytes, Pflüqers Arch., 412: 248–252.CrossRefGoogle Scholar
  4. Isenberg, G. and Klockner, U., 1982, Calcium tolerant ventricular myocytes prepared by preincubation in a “KB Medium”, Pflügers Arch., 395: 6–18.PubMedCrossRefGoogle Scholar
  5. Ishihara, H., Martin, B.L., Brautigan, D.L., Karaki, H., Ozaki, H., Kato, Y., Fusetani, N., Watabe, S., Hashimoto, K., Uemura, D. and Hartshorne, D.J., 1989, Calyculin A and okadaic acid: Inhibitors of protein phosphatase activity, Biochem. Biophys. Res. Commun., 159: 871–877.PubMedCrossRefGoogle Scholar
  6. Ishihara, H., Ozaki, H., Sato, K., Hori, M., Karaki, H., Watabe, S., Kato, Y., Fusetani, N., Hashimoto, K., Uemura, D. and Hartshorne, D.J., 1989, Calcium-independent activation of contractile apparatus in smooth muscle by calyculin-A, J. Pharmacol. Exp. Ther., 250: 388–396.PubMedGoogle Scholar
  7. Kameyama, M., Hescheler, J., Hofmann, F. and Trautwein, W., 1986, Modulation of Ca current during the phosphorylation cycle in the guinea pig heart, Pflügers Arch., 407: 123–128.PubMedCrossRefGoogle Scholar
  8. Kato, Y., Fusetani, N., Matsunaga, S. and Hashimoto, K., 1986, Calyculin A, a novel antitumor metabolite from the marine sponge Discodermia calyx, J. Am. Chem. Soc., 108: 2780–2781.CrossRefGoogle Scholar
  9. Obara, K., 1984, Isolation and contractile properties of single smooth muscle cells from guinea pig taenia caeci, Jpn. J. Physiol., 34: 41–54.PubMedCrossRefGoogle Scholar
  10. Ohya, Y., Kitamura, K. and Kuriyama, H., 1987, Modulation of ionic currents in smooth muscle balls of the rabbit intestine by intracellular perfused ATP and cyclic AMP, Pflügers Arch., 408: 465–473.PubMedCrossRefGoogle Scholar
  11. Ozaki, H., Kojima, T., Moriyama, T., Karaki, H., Urakawa, N., Kohama, K. and Nonomura, Y., 1987, Inhibition by amiloride of contractile elements in smooth muscle of guinea pig taenia cecum and chicken gizzard, J. Pharmacol. Exp. Ther., 243: 370–377.PubMedGoogle Scholar
  12. Yabu, H., Yoshino, M., Usuki, T., Someya, T., Obara, K., Ozaki, H. and Karaki, H., 1990, Modification by calyculin A of inward Ca currents in smooth muscle cells isolated from guinea pig taenia coli, in: “Frontiers in Smooth Muscle Research”, N. Sperelakis and J.D. Wood, eds., pp623–626, Wiley-Liss, New York.Google Scholar

Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Hideyo Yabu
    • 1
  • Kazuo Obara
    • 1
  • Toshihiro Usuki
    • 1
  1. 1.Department of PhysiologySapporo Medical CollegeSapporoJapan

Personalised recommendations