Effect of Ryanodine on Glycerinated Rabbit Psoas Muscle Fibers
It is well known that contraction and relaxation in skeletal muscle are regulated by Ca2+ release from and re-uptake by SR, and the latter to be an ATP-dependent Ca2+ pump (Ikemoto, 1982). On the other hand, release of Ca2+ is the key problem less well understood in E-C coupling, and the process occur in the absence of ATP, but not a simple reversal of Ca ion sequestration. In this study, we utilized a biphasic contracture of glycerinated fiber which showing an interaction of both processes. Thus, to assess the role of ATP in the mechanism of E-C coupling, effects of ryanodine, ruthenium red, nucleoside triphosphate (NTP), and the nonhydrolyzable ATP analog (AMP-PNP) on ATP- and Ca-induced contractures of glycerinated rabbit psoas muscle fiber were studied. Results suggest that ATP affects Ca release in the sarcoplasmic reticulum.
KeywordsRuthenium Nucleoside Triphosphate EGTA Berman
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