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Effect of Ryanodine on Glycerinated Rabbit Psoas Muscle Fibers

  • Takeshi Tsujimoto
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 311)

Abstract

It is well known that contraction and relaxation in skeletal muscle are regulated by Ca2+ release from and re-uptake by SR, and the latter to be an ATP-dependent Ca2+ pump (Ikemoto, 1982). On the other hand, release of Ca2+ is the key problem less well understood in E-C coupling, and the process occur in the absence of ATP, but not a simple reversal of Ca ion sequestration. In this study, we utilized a biphasic contracture of glycerinated fiber which showing an interaction of both processes. Thus, to assess the role of ATP in the mechanism of E-C coupling, effects of ryanodine, ruthenium red, nucleoside triphosphate (NTP), and the nonhydrolyzable ATP analog (AMP-PNP) on ATP- and Ca-induced contractures of glycerinated rabbit psoas muscle fiber were studied. Results suggest that ATP affects Ca release in the sarcoplasmic reticulum.

Keywords

Sarcoplasmic Reticulum Release Channel Nucleoside Triphosphate Fast Skeletal Muscle Terminal Cisterna 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Takeshi Tsujimoto
    • 1
  1. 1.Department of PhysiologyWakayama Medical CollegeKyuban-cho, WakayamaJapan

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