Effect of Ryanodine on Glycerinated Rabbit Psoas Muscle Fibers

  • Takeshi Tsujimoto
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 311)


It is well known that contraction and relaxation in skeletal muscle are regulated by Ca2+ release from and re-uptake by SR, and the latter to be an ATP-dependent Ca2+ pump (Ikemoto, 1982). On the other hand, release of Ca2+ is the key problem less well understood in E-C coupling, and the process occur in the absence of ATP, but not a simple reversal of Ca ion sequestration. In this study, we utilized a biphasic contracture of glycerinated fiber which showing an interaction of both processes. Thus, to assess the role of ATP in the mechanism of E-C coupling, effects of ryanodine, ruthenium red, nucleoside triphosphate (NTP), and the nonhydrolyzable ATP analog (AMP-PNP) on ATP- and Ca-induced contractures of glycerinated rabbit psoas muscle fiber were studied. Results suggest that ATP affects Ca release in the sarcoplasmic reticulum.


Sarcoplasmic Reticulum Release Channel Nucleoside Triphosphate Fast Skeletal Muscle Terminal Cisterna 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Bianchi, C.P. and Narayan, S., 1990, Bimodal operation of the ryanodine-sensitive transducer calcium channel, Toxicon, 28:1173–1181.PubMedCrossRefGoogle Scholar
  2. Endo, M. and Kitazawa, T., 1976, The effect of ATP on calcium release mechanisms in the sarcoplasmic reticulum of skinned muscle fibers, Proc. Japan Acad., 52:595–598.Google Scholar
  3. Fleischer, S., Ogunbunmi, E.M., Dixon, M.C. and Fleer, E.A.M., 1985, Localization of Ca2+ release channels with ryanodine in junctional terminal cisternae of sarcoplasmic reticulum of fast skeletal muscle, Proc. Natl. Acad. Sci., 82:7256–7259.PubMedCrossRefGoogle Scholar
  4. Ikemoto, N., 1982, Structure and function of the calcium pump protein of sarcoplasmic reticulum, Ann. Rev. Physiol., 44:297–317.CrossRefGoogle Scholar
  5. Meissner, G., 1986, Ryanodine activation and inhibition of the Ca2+ release channel of sarcoplasmic reticulum, J. Biol. Chem., 261:6300–6306.PubMedGoogle Scholar
  6. Reuben, J.P., Brandt, P.W., Berman, M. and Grundfest, H., 1971, Regulation of tension in the skinned crayfish muscle fiber I. Contraction and relaxation in the absence of Ca (pCa>9), J. Gen. Physiol., 57:385–407.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Takeshi Tsujimoto
    • 1
  1. 1.Department of PhysiologyWakayama Medical CollegeKyuban-cho, WakayamaJapan

Personalised recommendations