Effect of Ryanodine on Glycerinated Rabbit Psoas Muscle Fibers
It is well known that contraction and relaxation in skeletal muscle are regulated by Ca2+ release from and re-uptake by SR, and the latter to be an ATP-dependent Ca2+ pump (Ikemoto, 1982). On the other hand, release of Ca2+ is the key problem less well understood in E-C coupling, and the process occur in the absence of ATP, but not a simple reversal of Ca ion sequestration. In this study, we utilized a biphasic contracture of glycerinated fiber which showing an interaction of both processes. Thus, to assess the role of ATP in the mechanism of E-C coupling, effects of ryanodine, ruthenium red, nucleoside triphosphate (NTP), and the nonhydrolyzable ATP analog (AMP-PNP) on ATP- and Ca-induced contractures of glycerinated rabbit psoas muscle fiber were studied. Results suggest that ATP affects Ca release in the sarcoplasmic reticulum.
KeywordsSarcoplasmic Reticulum Release Channel Nucleoside Triphosphate Fast Skeletal Muscle Terminal Cisterna
- Endo, M. and Kitazawa, T., 1976, The effect of ATP on calcium release mechanisms in the sarcoplasmic reticulum of skinned muscle fibers, Proc. Japan Acad., 52:595–598.Google Scholar