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Control of Calcium Release from the Sarcoplasmic Reticulum

  • G. D. Lamb
  • D. G. Stephenson
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 311)

Abstract

In order to study many of the important events in excitation-contraction (E-C) coupling in skeletal muscle, it is necessary to have access to the intracellular environment while still retaining the normal voltage-sensor control over Ca2+ release from the sarcoplasmic reticulum (SR). We have achieved this using a skinned fibre preparation similar to that described previously (Donaldson, 1985; Stephenson, 1985; Fill & Best, 1988), with modifications that improve the viability of the preparation and enable normal E-C coupling to be distinguished from non-physiological Ca2+ release that can occur. We review here some of the more important findings obtained with this preparation.

Keywords

Sarcoplasmic Reticulum Bathing Solution Calcium Release Release Channel Skeletal Muscle Fibre 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • G. D. Lamb
    • 1
  • D. G. Stephenson
    • 1
  1. 1.Department of ZoologyLa Trobe UniversityMelbourneAustralia

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