Control of Calcium Release from the Sarcoplasmic Reticulum
In order to study many of the important events in excitation-contraction (E-C) coupling in skeletal muscle, it is necessary to have access to the intracellular environment while still retaining the normal voltage-sensor control over Ca2+ release from the sarcoplasmic reticulum (SR). We have achieved this using a skinned fibre preparation similar to that described previously (Donaldson, 1985; Stephenson, 1985; Fill & Best, 1988), with modifications that improve the viability of the preparation and enable normal E-C coupling to be distinguished from non-physiological Ca2+ release that can occur. We review here some of the more important findings obtained with this preparation.
KeywordsSarcoplasmic Reticulum Bathing Solution Calcium Release Release Channel Skeletal Muscle Fibre
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