New Approaches to the Measurement of Proliferation Rates

  • Juliana Denekamp
Part of the NATO ASI Series book series (NSSA, volume 227)

Abstract

The field of cell proliferation kinetics has expanded enormously since the original description in 1953 that DNA synthesis in preparation for the next mitosis occupies a discrete portion of the intermitotic life cycle. Howard & Pelc (1953) defined the phases of the cell cycle, i.e. the postmitotic gap (G1), DNA synthesis (S) and the premitotic gap (G2). This nomenclature has become very commonly adopted. Specific precursors of DNA have been developed which allow cells in this phase of DNA synthesis to be labelled. The most extensively used has been radioactive tritiated thymidine, but this is now being replaced by the non-radioactive halogenated pyrimidines BUdR and IUdR. The radioactive precursors were detected in autoradiographs by the grains they induce in a photographic emulsion as the isotope decays. The overall uptake can also be detected in a scintillation counter. By contrast the BUdR or IUdR labelled cells can be detected with specific monoclonal antibodies, which can then be tagged with coloured or fluorescent stains and identified in histological sections or by flow cytometry (Gray et al. 1986).

Keywords

Bromide Radioactive Isotope Sarcoma Iodide Ethidium 

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References

  1. Begg, A.C., McNally, N.J., Shrieve, D.C., Kärcher, H. A method to measure the duration of DNA synthesis and the potential doubling time from a single sample. Cytometry 6: 620–626. 1985.PubMedCrossRefGoogle Scholar
  2. Begg, A.C. Derivation of cell kinetic parameters from human tumours after labelling with bromodeoxyuridine or iododeoxyuridine. Brit.J.Radiol. Report 19: 113–119. 1989.Google Scholar
  3. Denekamp, J. Cell kinetics and Cancer Therapy, pp.1–162. Pub. C.C. Thomas, USA. 1982.Google Scholar
  4. Gray, J.W., Darzynkiewicz (editors). Techniques in Cell Cycle Analysis, pp.1–407. Humana Press. Clifton N.J., USA. 1986.Google Scholar
  5. Howard, A. and Pelc, S.R. Synthesis of desoxyribonucleic acid in normal and irradiated cells and its relation to chromosome breakage. Heredity, suppl.6: 261–273. 1953.Google Scholar
  6. Steel, G.G. Growth kinetics of tumours. Oxford University Press. 1977.Google Scholar
  7. Wilson, G.D., McNally, N.J., Dische, S., Bennett, M.H. Cell proliferation in human tumours measured by in vivo labelling with bromodeoxyuridine. Brit.J.Radiol. 61: 419–422. 1988.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Juliana Denekamp
    • 1
  1. 1.CRC Gray LaboratoryNorthwood MiddlesexUK

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