Skip to main content
SpringerLink
Log in
Book cover

Analysis of Antibiotic/Drug Residues in Food Products of Animal Origin pp 147–163Cite as

Comparison of Chromatographic Procedures for Determining Residues of Penicillins

  • Chapter

Abstract

Residues of penicillins in milk or tissues are traditionally analyzed by microbiological tests. However, these techniques can give false-positive results caused by active metabolites or antibacterial endo- or exogenic substances that may interfere in microbiological methods. Another drawback is poor precision. While bioassay procedures can distiguish resulting penicillin residues from other antibiotics by use of penicillinase, they cannot distinguish penicillins from one other. By the introduction of chromatographic procedures it is possible to separate and identify different penicillins. The main problem is that if they are to be of any value as confirmatory methods they must equal or exceed the sensitivity of the microbiological screening tests at least as long as maximum residue limits are set at these levels.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   99.00
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. C. D. Salisbury, C. E. Rigby, and W. Chan, Determination of antibiotic residues in Canadian slaughter animals by thin-layer chromatography-bioautography, J. Agric. Food Chem 37:105 (1989).

    Article  CAS  Google Scholar 

  2. R. K. Munns, W. Shimoda, J. E. Roybal, and C. Vieira, Multiresidue method for determination of eight neutral ß-lactam penicillins in milk by fluorescence-liquid chromatography, J. Assoc. Off. Anal. Chem 68:968 (1985).

    PubMed  CAS  Google Scholar 

  3. U. Meetschen and M. Petz, Capillary gas chromatographic method for determination of benzylpenicillin and other beta lactam antibiotics in milk, J. Assoc. Off. Anal. Chem 73:373 (1990).

    PubMed  CAS  Google Scholar 

  4. U. Meetschen and M. Petz, Gas chromatographic method for the determination of residues of seven penicillins in foodstuffs of animal origin, Z. Lebensm. Unters. Forsch. 194 (4) (1991) in print.

    Google Scholar 

  5. S. A. Barker, A. R. Long and C. R. Short, Isolation of drug residues from tissues by solid phase dispersion, J. Chromatogr 475:353 (1989).

    Article  PubMed  CAS  Google Scholar 

  6. K. Tyczkowska, R. D. Voyksner, and A. L. Aronson, Development of an analytical method for penicillin G in bovine milk by liquid chromatography with ultraviolet-visible detection and confirmation by mass-spectrometric detection, J. Chromatogr 490:101 (1989).

    Article  PubMed  CAS  Google Scholar 

  7. H. Terada, M. Asanoma, and Y. Sakabe, III. High-performance liquid chromatographic determination of penicillin G in animal tissues using an on-line pre-column concentration and purification system, J. Chromatogr 318:299 (1985).

    Article  PubMed  CAS  Google Scholar 

  8. W. A. Moats, Determination of penicillin G in milk by high-performance liquid chromatography with automated liquid chromatographic cleanup, J. Chromatogr 507:177 (1990).

    Article  PubMed  CAS  Google Scholar 

  9. H. Lingeman, R. D. McDowall, and U. A. Th. Brinkman, Guidelines for bioanalysis using column liquid chromatography, Trends in Analytical Chemistry 10:48 (1991).

    Article  CAS  Google Scholar 

  10. W. A. Moats, Detection and semiquantitative estimation of penicillin G and cloxacillin in milk by thin-layer chromatography, J. Agric. Food Chem. 31:1348 (1983).

    Article  CAS  Google Scholar 

  11. B. Wiese and K. Martin, Determination benzylpenicillin in milk at the pg m1-1 level by reversed-phase liquid chromatography in combination with digital subtraction chromatography technique, J. Pharm. Biomed Anal. 7:95 (1989).

    Article  PubMed  CAS  Google Scholar 

  12. H. Terada and Y. Sakabe, IV. Simultaneous determination of penicillin G, penicillin V and ampicillin in milk by high-performance liquid chromatography, J. Chromatogr 348:379 (1985).

    Article  PubMed  CAS  Google Scholar 

  13. J. O. Boison, C. D. C. Salisbury, W. Chan, and J. D. MacNeil, Determination of penicillin G residues in edible animal tissues by liquid chromatography, J. Assoc. Off. Anal. Chem 74:497 (1991).

    PubMed  CAS  Google Scholar 

  14. J. O. Miners, The analysis of penicillins in biological fluids and pharmaceutical preparations by high-performance liquid chromatography: A review, J. Liquid Chromatogr 8:2827 (1985).

    Article  CAS  Google Scholar 

  15. B. Wiese and K. Martin, Basic extraction studies of benzylpenicillin and its determination by liquid chromatography with pre-column derivatisation, J. Pharm.,Biomed. Anal 7:67 (1989).

    Article  CAS  Google Scholar 

  16. F. Nachtmann and K. Gstrein, Simultaneous determination of the cationic and anionic parts in respiratory penicillins by high-performance liquid chromatography, J. Chromatogr 236:461 (1982).

    Article  CAS  Google Scholar 

  17. U. R. Tjaden, H. Lingeman, R. A. M. van der Hoeven, J. A. C. Bierman, H. J. E. M. Reeuwijk, and J. van der Greef, High-performance liquid chromatographic analysis of isoxazolylpenicillins in plasma and urine samples, Chromatographia 24:597 (1987).

    Article  CAS  Google Scholar 

  18. J. Carlqvist and D. Westerlund, Automated determination of amoxycillin in biological fluids by column switching in ion-pair reversed-phase liquid chromatographic systems with post-column derivatization, J. Chromatogr 344:285 (1985).

    Article  PubMed  CAS  Google Scholar 

  19. H. Mascher and C. Kikuta, Determination of amoxicillin in plasma by high-performance liquid chromatography with fluorescence detection after on-line oxidation, J. Chromatogr 506:417 (1990).

    Article  PubMed  CAS  Google Scholar 

  20. A. J. Shah and M. W. Adlard, Determination of ß-lactams and their biosynthetic intermediates in fermentation media by pre-column derivatisation followed by fluorescence detection, J. Chromatogr 424:324 (1988).

    Google Scholar 

  21. M. E. Rogers, M. W. Adlard, G. Saunders, and G. Holt, Derivatization techniques for high-performance liquid chromatographic analysis of ß-lactams, J. Chromatogr 297:385 (1984).

    Article  CAS  Google Scholar 

  22. K. Iwaki, N. Okumura, M. Yamazaki, N. Nimura, and T. Kinoshita, Precolumn derivatization technique for high-performance liquid chromatographic determination of penicillins with fluorescence detection, J. Chromatogr 504:359 (1990).

    Article  PubMed  CAS  Google Scholar 

  23. R. K. Munns, J. E. Roybal, W. Shimoda, and J. A. Hurlbut, 1-(4-Hydroxyphenyl)-, 1-(2,4-dihydroxyphenyl)- and l-(2,5-dihydroxyphenyl)-2bromoethanones: New labels for determination of carboxylic acids by high-performance liquid chromatography with electrochemical and ultraviolet detection, J. Chromatogr 442:209 (1988).

    Article  PubMed  CAS  Google Scholar 

  24. C. M. Selavka, I. S. Krull, and K. Bratin, Analysis for penicillins and cefoperazone by HPLC-photolysis-electrochemical detection (HPLC-hvEC), J. Pharm. Biomed. Anal 4:83 (1986).

    Article  PubMed  CAS  Google Scholar 

  25. K. Berger and M. Petz, Fluorescence HPLC determination of penicillins using 4-bromomethyl-7-methoxycoumarin as pre-column labelling agent, Dtsch. Lebensm. Rundsch 87:137 (1991).

    CAS  Google Scholar 

  26. W. A. Moats, Effects of the silica support of bonded reversed-phase columns on chromatography of some antibiotic compounds, J. Chromatogr. 366:69 (1986).

    Article  PubMed  CAS  Google Scholar 

  27. W. A. Moats and L. Leskinen, Comparison of bonded, polymeric and silica columns for chromatography of some penicillins, J. Chromatogr 386:79 (1987).

    Article  PubMed  CAS  Google Scholar 

  28. A. M. Lipczynski, Reversed-phase high-performance liquid chromatographic retention behaviour of benzylpenicillin and its acid-base degradation products, Analyst 112:411 (1987).

    Article  PubMed  CAS  Google Scholar 

  29. J. Martin, R. Mendez, and A. Negro, Effect of temperature on HPLC separations of penicillins, J. Liquid Chromatogr 11:1707 (1988).

    Article  CAS  Google Scholar 

  30. P. C. Van Krimpen, W.P. van Bennekom, and A. Bult, Penicillins and cephalosporins - Physicochemical properties and analysis in pharmaceutical and biological matrices, Pharm. Weekbl. Sci. Ed 9:1 (1987).

    Article  Google Scholar 

  31. J. Hamann, A. Tolle, A. Blüthgen, and W. Heeschen, Studies on detection, isolation and identification of antibiotic residues in milk. 1. Isoxazolyl penicillins, Milchwissenschaft 30:1 (1975).

    CAS  Google Scholar 

  32. W. A. Moats, Determination of penicillin G, penicillin V, and cloxacillin in milk by reversed-phase high-performance liquid chromatography, J. Agric. Food Chem 31:880 (1983).

    Article  CAS  Google Scholar 

  33. T. Nagata and M. Saeki, Determination of ampicillin residues in fish tissues by liquid chromatography, J. Assoc. Off. Anal. Chem 69:448 (1986).

    PubMed  CAS  Google Scholar 

  34. U. Meetschen, Gas chromatographic method for residues of seven penicillins in foodstuffs of animal origin. Thesis, University of Münster/Germany, 1990.

    Google Scholar 

  35. W.A. Moats, Determination of pencillin G and cloxacillin residues in beef and pork tissue by high-performance liquid chromatography, J. Chromatogr 317:311 (1984).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Food Chemistry — FB 9, University of Wuppertal, Gaußstraße 20, D-5600, Wuppertal 1, USA

    Michael Petz

Authors
  1. Michael Petz
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. The Connecticut Agricultural Experiment Station, New Haven, Connecticut, USA

    Vipin K. Agarwal

Rights and permissions

Reprints and permissions

Copyright information

© 1992 Springer Science+Business Media New York

About this chapter

Cite this chapter

Petz, M. (1992). Comparison of Chromatographic Procedures for Determining Residues of Penicillins. In: Agarwal, V.K. (eds) Analysis of Antibiotic/Drug Residues in Food Products of Animal Origin. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3356-6_12

Download citation

  • DOI: https://doi.org/10.1007/978-1-4615-3356-6_12

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6480-1

  • Online ISBN: 978-1-4615-3356-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation