Abstract
Alcohol interferes with amino acid and protein metabolism at various levels in different tissues. Effects of ethanol on the digestion of exogenous proteins and the absorption of their constituent amino acids is discussed in Chapter 10. In this chapter we focus primarily on the effects of ethanol on amino acid metabolism, the endogenous synthesis of proteins (primarily in the liver), their degradation, and their secretion from the liver into the bloodstream. Hepatic consequences of the alterations in these processes are emphasized.
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References
Abrams, M. A., and Cooper, C.: Quantitative analysis of metabolism of hepatic triglyceride in ethanol treated rats. Biochem. J. 156:33–46, 1976.
Adibi, S. A.: Interrelationships between levels of amino acids in plasma and tissues during starvation. Am. J. Physiol. 221: 829–838, 1971.
Adibi, S. A., Modesto, T. A., Morse, E. L., and Amin, P. M.: Amino acid levels in plasma liver and skeletal muscle during protein deprivation. Am. J. Physiol. 225:408–414, 1973.
Albertini, A., Fiaccavento, S., and Bonera, B.: Turnover of liver ribosomes in ethanol-intoxicated rats. Chem. Biol. Interact. 2:57–60, 1970.
Algramati, I. D., and Sabatini, D. D.: Effect of protease inhibitors on albumin secretion in hepatoma cells. Biochem. Biophys. Res. Commun. 90:220–226, 1979.
Ashworth, C. T., Johnson, C. F., and Wrightman, F J.: Hepatic composition and morphologic correlations of hepatic protein synthesis in acute ethanol intoxication in rats. Am. J. Pathol. 46:757–773, 1965.
Avogaro, A., Cibin, M., Croatto, T., Rizzo, A., Gallimbert, L., and Tiengo, A.: Alcohol intake and withdrawal: Effects on branched-chain amino acids and alanine. Alcoholism: Clin. Exp. Res. 10:300–304, 1986.
Banks, W. L., Kline, E. S., and Higgins, E. S.: Hepatic composition and metabolism after ethanol consumption in rats fed liquid purified diets. J. Nutr. 100:581–593, 1970.
Baranyai, J. M., and Blum, J. J.: Quantitative analysis of intermediary metabolism in rat hepatocytes incubated in the presence and absence of ethanol with a substrate mixture including ketoleucine. Biochem. J. 258:121–140, 1989.
Baraona, E., and Lieber, C. S.: Effects of chronic ethanol feeding on serum lipoprotein metabolism in the rat. J. Clin. Invest. 49:769–778, 1970.
Baraona, E., and Lieber, C. S.: Effects of ethanol on hepatic protein synthesis and secretion. In: Currents in Alcoholism, Vol. 1 (F A. Seixas, ed.), New York, Grune & Stratton, pp. 715, 1977.
Baraona, E., and Lieber, C. S.: Effects of alcohol on hepatic transport of proteins. Annu. Rev. Med. 33:281–292, 1982.
Baraona, E., and Lieber, C. S.: Effects of ethanol on hepatic protein metabolism. In: Alcohol and Protein Synthesis: Ethanol, Nucleic Acid, and Protein Synthesis in the Brain and Other Organs, NIAAA Research Monograph-10, DHHS publication No. (ADM) 83–1198, pp. 75–95, 1983.
Baraona, E., Pirola, R. C., and Lieber, C. S.: Pathogenesis of postprandial hyperlipemia in rats fed ethanol-containing diets. J. Clin. Invest. 52:296–303, 1973.
Baraona, E., Leo, M. A., Borowsky, S. A., and Lieber, C. S.: Alcoholic hepatomegaly: Accumulation of protein in the liver. Science 190:794–795, 1975.
Baraona, E., Leo, M. A., Borowsky, S. A., and Lieber, C. S.: Pathogenesis of alcohol-induced accumulation of protein in the liver. J. Clin. Invest. 60:546–554, 1977.
Baraona, E., Matsuda, Y., Pikkarainen, P, Finkelman, F, and Lieber, C. S.: Exaggeration of the ethanol-induced decrease in liver microtubules after chronic alcohol consumption. Gastroenterology 76:1274, 1979.
Baraona, E., Pikkarainen, P, Salaspuro, M., Finkelman, E, and Lieber, C. S.: Acute effects of ethanol on hepatic protein synthesis and secretion in the rat. Gastroenterology 79:104–111, 1980.
Baraona, E., Matsuda, Y., Pikkarainen, P., Finkelman, F., and Lieber, C. S.: Effects of ethanol on hepatic protein secretion and microtubules. Possible mediation by acetaldehyde. Curr. Alcohol. 8:421–434, 1981a.
Baraona, E., Guerra, M., and Lieber, C. S.: Cytogenetic damage of bone marrow cells produced by chronic alcohol consumption. Life Sci. 29:1797–1802, 1981b.
Baraona, E., Shaw, S., and Lieber, C. S.: Alcohol-induced changes of amino acid and protein metabolism. In: Medical Disorders of Alcoholism: Pathogenesis and Treatment (C. S. Lieber, ed.), Philadelphia, Saunders, pp. 178–236, 1982.
Baraona, E., Jauhonen, P., Miyakawa, H., and Lieber, C. S.: Zonal redox changes as a cause of selective perivenular hepatotoxicity of alcohol. Pharmacol. Biochem. Behan 18:449–454, 1983.
Baraona, E., Finkelman, F., and Lieber, C. S.: Reevaluation of the effects of alcohol consumption on rat liver microtubules: Effect of feeding status. Res. Commun. Chem. Path. Pharmacol. 44:265–278, 1984.
Bass, N. M.: Function and regulation of hepatic and intestinal fatty acid binding protein. Chem. Phys. Lipids. 38:95–114, 1985.
Beesley, R. C.: Ethanol inhibits Na -gradient-dependent uptake of t.-amino acids into intestinal brush border membrane vesicles. Dig. Dis. Sci. 31:987–992, 1986.
Behrens, U. J., Worner, T. M., Braly, L. F, Schaffner, F., and Lieber, C. S.: Carbohydrate-deficient transferrin (CDT), a marker for chronic alcohol consumption in different ethnic populations. Alcoholism: Clin. Exp. Res. 12:427–432, 1988a.
Behrens, U. J., Worner, T. M., and Lieber, C. S.: Changes in carbohydrate deficient transferrin (CDT) levels after alcohol withdrawal. Alcoholism: Clin. Exp. Res. 12:539–544, 1988b.
Bengtsson, G., Smith-Kielland, A., and Morland, J.: Ethanol effects on protein synthesis in nonparenchymal liver cells, hepatocytes, and density populations of hepatocytes. Exp. Mol. Pathol. 41:44–57, 1984.
Berman, W. J., Gil, J., Jennett, R. B., Tuma, D., Sorrell, M. F., and Rubin, E.: Ethanol, hepatocellular organelles, and micro-tubules. A morphometric study in vivo and in vitro. Lab. Invest. 48:760–767, 1983.
Bhuyan, U. N., Nayak, N. C., Deo, M. G., and Ramalingaswami, V.: Effect of dietary protein on carbon tetrachloride-induced hepatic fibrogenesis in albino rats. Lab. Invest. 14:184–190, 1965.
Blank, G. S., Yaffe, M. B., Szasz, J., George, E., Rosenberry, T. L., and Sternlicht, H.: The role of Lys 394 in microtubule assembly. Ann. NY Acad. Sci. 466:467–481, 1986.
Borisy, G. G.: A rapid method for quantitative determination of microtubule protein using DEAE-cellulose filters. Anal. Biochem. 50:373–385, 1972.
Branchey, L., and Lieber, C. S.: Activation of tryptophan pyrrolase after chronic alcohol administration. Subst. and Alcohol ActionslMisuse 2:225–229, 1982.
Branchey, L., Shaw, S., Lieber, C. S.: Ethanol impairs tryptophan transport into the brain and depresses serotonin. Life. Sci. 29:2751–2755, 1981.
Burke, J. P.,Tumbelson, M. E., Hicklin, K. W, and Wilson, R. B.: Effect of chronic ethanol ingestion on mitochondrial protein synthesis in Sinclair (s-1) miniature swine. Proc. Soc. Exp. Biol. Med. 148:1051–1056, 1974.
Burnett, D. A.: Paradoxical effect of ethanol on hepatic tri- glyceride secretion. Gastroenterology 77:A-6, 1979.
Buydens-Branchey, L., Branchey, M., Worner, T. M., Zucker, D., Aramsobatdee, E., and Lieber, C. S.: Increase in tryptophan oxygenase activity in alcoholic patients. Alcoholism: Clin. Exp. Res. 12:163–168, 1988.
Carulli, N., Manenti, F., Gallo, M., and Salvioli, G. F.: Alcohol-drugs interactions in man: Alcohol and tolbutamide. Eur. J. Clin. Invest. 1:421–424, 1971.
Cerdan, S., Robles, S. S., Ayuso, M. S., and Parrilla, R.: Acute effects of ethanol on hepatic protein synthesis in the rat in vivo. IRCS’Med. Sci. 8:229, 1980.
Cerdan, S., Sanchez, S., Martin-Requero, A., Ayuso-Parrilla, M. S., and Parrilla, R.: Role of the state of reduction of the NAD system on the regulation of hepatic protein synthesis in the rat in vivo. Intl. J. Biochem. 14:615–620, 1982.
Chambers, J. W, and Piccirillo, V. J.: Effects of ethanol on amino-acid uptake and utilization by the liver and other organs of rats. Q. J. Stud. Alcohol 34:707–717, 1973.
Chambers, J. W, Georg, R. H., and Bass, A. D.: The effect of ethanol on the uptake of a-aminoisobutyric acid by the isolated perfused rat liver. Life Sci. 5:2293–2300, 1966.
Chang, T., Lewis, J., and Glazko, A. J.: Effect of ethanol and other alcohols on the transport of amino acids and glucose by everted sacs of rat small intestine. Biochim. Biophys. Acta 135:1000–1007, 1967.
Combcscoc, C., Courte, M.-T., Rcynouard, E, and Weill, J.: Modifications de l’incorporation de l’uridine tritée dans l’acide ribonucléique hépatique du rat Wistar après l’administration prologée per os d’éthanol. C. R. Soc. Biol. 163:2410–2412, 1969.
Courtoy, P J., Lombart, C., Feldman, G., Rogier, E., and Moguilevsky, N.: Synchronous increase in four acute phase proteins synthesized by the same hepatocytes during the inflammatory reaction. A combined biochemical and morphologic kinetics study in the rat. Lab. Invest. 44:105–115, 1981.
Criss, W. E.: A review of isozymes in cancer. Cancer Res. 31: 1523–1542, 1971.
Criss, W. E.: Control of the adenylate charge in the Morris “minimal-deviation” hepatomas. Cancer Res. 33:51–56, 1973.
Crow, K. E., Cornell, N. W., Veech, R. L.: The rate of ethanol metabolism in isolated rat hepatocytes. Alcohlism: Clin. Exp. Res. 1:43–47, 1977.
Cruz, M. A., Bravo, I., Rojas, S., and Gallardo, V: Effects of ethanol ingestion on amino acid uptake in the dog liver in vivo. Pharmacology 30:12–19, 1985.
Dajani, R. M., and Kouyoumjian, R.: A probable direct role of ethanol in the pathogenesis of fat infiltration in the rat. J. Nutr. 91:535–539, 1967.
David, E. T., Fischer, I., and Moldave, K.: Studies on the effect of ethanol on eukaryotic protein synthesis in vitro. J. Biol. Chem. 258:7702–7706, 1983.
Denk, H., and Eckerstorfer, R.: Colchicine-induced Mallory body formation in the mouse. Lab. Invest. 36:563–565, 1977.
Denk, H., Gschnait, F., and Wolff, K.: Hepatocellular hyalin (Mallory bodies) in long term griseofulvin-treated mice: A new experimental model for study of hyaline formation. Lab. Invest. 32:773–776, 1975.
Denk, H., Franke, W. W, Dragosics, B., and Zeiler, I.: Pathology of cytoskeleton of liver cells: Demonstration of Mallory bodies (alcoholic hyalin) in murine and human hepatocytes by immunofluorescent microscopy using antibodies to cytokeratin polypeptides from hepatocytes. Hepatology 1:9–20, 1981.
Dich, J., and Tdnnesen, I. C.: Effects of ethanol, nutritional status, and composition of the incubation medium on protein synthesis in isolated rat liver parenchymal cells. Arch. Biochem. Biophys. 204:640–647, 1980.
Dinda, P. K., and Beck, I. T.: Effect of ethanol on peptidases of hamster jejunal brush-border membrane. Am. J. Physiol. 242:G442–G447, 1982.
Domschke, S., Domschke, W, and Lieber, C. S.: Hepatic redox state: Attenuation of the acute effects of ethanol induced by chronic ethanol consumption. Life Sci. 15:1327–1334, 1974.
Donohue, T. M., Sorrell, J. H., Sorrell, M. F., and Tuma, D. J.: Measurement of protein synthetic activity by determination of peptidyl[3H]puromycin formation in liver slices after ethanol administration. Alcoholism: Clin. Exp. Res. 9:526–530, 1985.
Donohue, T M., Sorrell, M. F., and Tuma, D. J.: Hepatic protein synthetic activity in vivo after ethanol administration. Alco holism: Clin. Exp. Res. 11:80–86, 1987.
Donohue, T M., Zetterman, R. K., and Tuma, D. J.: Effect of chronic ethanol administration on protein catabolism in rat liver. Alcoholism: Clin. Exp. Res. 13:49–57, 1989a.
Donohue, T. M., Chaisson, M. L., Zetterman, D., Krako, J. A., and Zetterman, R. K.: Effects of ethanol on catabolism of plasma proteins. Alcoholism: Clin. Exp. Res. 13:326, 1989b.
Dorio, R. J., Hoek, J. B., and Rubin, E.: Ethanol treatment selectively decreases neutral amino acid transport in cultured hepatocytes. J. Biol. Chem. 259:11430–11435, 1984.
Dunlop, D. S., Van Elden, W., and Lajtha, A.: A method for measuring brain protein synthesis rates in young adult rats. J. Neurochem. 24:337–344, 1975.
Edwards, K., Nagashima, M., Dryburgh, H., Wykes, A., and Schreiber, G.: Secretion of protein from liver cells is suppressed by the proteinase inhibitor N-alpha-tosyl-L-lysyl chloromethane, but not by tunycamicin, an inhibitor of glycosylation. FEBS Lett. 100:269–272, 1979.
Eletsky, Y. K., and Merkulov, M. F.: The effect of ethanol on the 3H-uridine incorporation in RNA of murine hepatic cells. Farmakol. Toksikol. (Moskva) 32:561–564, 1969.
Elko, E. E., Wooles, W. R., and DiLuzio, N. R.: Alterations and mobilization of lipids in acute ethanol-treated rats. Am. J. Physiol. 201:923–926, 1961.
Estler, C. J.: Comparison of hepatic triglyceride content and hepatic lipid secretion after various doses of ethanol. Biochem. Pharmacol. 24:1871–1873, 1975.
Falk, M., Ahlberg, J., and Glaumann, H.: Ethanol intoxication stimulates lipolysis in isolated Golgi complex secretory vesicle fraction from rat liver. Virchows Arch. (Cell Pathol.) 49: 231–239, 1985.
Feldman, G., and Maurice, M.: Morphological findings of liver protein synthesis and secretion. In: Membrane Alterations or Basis of Liver Injury (H. Popper, L. Bianchi, and W. Reutter, eds.), Lancaster, MTP Press, p. 61, 1977.
Feldman, G., Koziner, B., Talamo, R., and Bloch, K. J.: Familial variable immunodeficiency: Autosomal dominant pattern as inheritance with variable expression of the defect(s). J. Pediatr. 87:534–539, 1975.
Fellenius, E., Carlgren, H., and Kiessling, H.-H.: Inhibition of glucogenesis from proline by ethanol oxidation. Life Sci. 13: 595–599,1973.
Fernandez-Checa, J. C., Ookhtens, M., and Kaplowitz, N.: Effect of chronic ethanol feeding on rat hepatocytic glutathione. Compartmentation, efflux, and response to incubation with ethanol. J. Clin. Invest. 80:57–62, 1987.
Fisher, S. E., Atkinson, M., Van Thiel, D. H., Rosenblum, E., David, R., and Holtzman, I.: Selective fetal malnutrition: Effect of ethanol and acetaldehyde upon in vitro uptake of a-aminoisobutyric by human placenta. Life Sci. 29:1283–1288, 1981.
Fisher, S. E., Barnicle, M. A., Steis, B., Holzman, I., and Van Thiel, D. H.: Effects of acute ethanol exposure upon in vivo leucine uptake and protein synthesis in the fetal rat. Pediatr. Res. 15:335–339, 1981.
Freedman, M. L., and Rosman, J.: A rabbit reticulocyte model for the role of hemin-controlled repressor in hypochromic anemias. J. Clin. Invest. 37:594–603, 1976.
Freedman, M. L., Cohen, H. S., Rosman, J., and Forte, F. J.: Ethanol inhibition of reticulocyte protein synthesis: The role of Haem. Br. J. Haematol. 30:351–363, 1975.
Freinkel, N., Arky, R. A., Singer, D. L., Cohen, A. K., Bleicher, S. J., Anderson, J. B., Silbert, C. K., and Foster, A. E.: Alcohol hypoglycemia IV. Current concepts of its pathogenesis. Diabetes 14:350–361, 1965.
French, S. W, Sim, J. S., Franks, K. E., Burbige, E. J., Denton, T., and Caldwell, M. G.: Alcoholic hepatitis. In: Alcoholic Hepatitis (M. M. Fisher and J. G. Rankin, eds.), New York, Plenum, 1977.
Gabriel, L., Bonelli, G., and Dianzani, M. U.: Inhibition of colchine binding to rat liver tubulin by aldehydes and by linoleic acid hydroperoxide. Chem. Biol. Interact. 19:101–109, 1977.
Gang, H., Lieber, C. S., and Rubin, E.: Ethanol increases glycosyl transferase activity in the hepatic Golgi apparatus. Nature [New Biol.] 243:123–125, 1973.
Gaub, J., Fauerholdt, L., Keidin, S., Kondrup, J., Petersen, P., and Wantzin, G. L.: Cytomorphometry of liver cells from ethanol-fed rats: ethanol causes increased polyploidization and protein accumulation. Eur. J. Clin. Invest. 11:235–237, 1981.
Girbes, T., Susin, A., Ayuso, M. S., and Parrilla, R. P: Acute effects of ethanol in the control protein synthesis in isolated rat liver cells. Arch. Biochem. Biophys. 226:37–49, 1983.
Glatz, J. F. C., and Veerkamp, J. H.: Intracellular fatty acid-binding proteins. Int. J. Biochem. 17:13–22, 1985.
Glaumann, H.: Studies on the synthesis and transport of albumin in microsomal subfractions from rat liver. Biochim. Biophys. Acta 224:206–218, 1970.
Glaumann, H., and Ericsson, J. L. E.: Evidence for the participation of the Golgi apparatus in the intracellular transport of nascent albumin in the liver cell. Cell Biol. 47:555–567, 1970.
Gordon, E. R.: Effect of an intoxication dose of ethanol on lipid metabolism in an isolated perfused rat liver. Biochem. Pharmacol. 21:2991–3004, 1972.
Green, R. S., MacDermid, R. G., Scheig, R. L., and Hajjar, J. J.: Effect of ethanol on amino acid absorption across in vivo rat intestine. Am. J. Physiol. 241:G176–G181, 1981.
Greenberger, N. J., Carley, J., Schenker, S., Bettinger, I., Stamnes, C., and Beyer, P: Effects of vegetable and animal protein diets in chronic hepatic encephalopathy. Dig. Dis. 22:845–855,1977.
Hajjar, J. J., Krasner, K., Van Linda, B., and Scheig, R.: Effect of ethanol on glycylsarcosine absorption. Life Sci. 14:1403–1407,1981a.
Hajjar, J. J. Tomicic, T., Scheig, R. L.: Effect of chronic ethanol consumption on leucine absorption in the rat small intestine. Digestion 22:170–176, 1981b.
Harbitz, I., Wallin, B., Hauge, J. G., and Morland, J.: Effect of ethanol metabolism on initiation of protein synthesis in rat hepatocytes. Biochem. Pharmacol. 33:3465–3470, 1984.
Heitman, D. W, Frosto, T A., Schenker, S., and Henderson, G. I.: Stimulatory effects of ethanol on amino acid transport by rat fetal hepatocytes. Hepatology 7:307–314, 1987.
Henderson, G. I., Hoyumpa, A. M., Rothschild, M. A., and Schenker, S.: Effect of ethanol and ethanol-induced hypothermia on protein synthesis in pregnant and fetal rats. Alcoholism: Clin. Exp. Res. 4:165–177, 1980.
Hirayama, C., and Hiroshige, K.: Effect of alpha-tocopherol and tocopheronolactone on ethanol induced fatty liver and triglyceridernia. Experientia 26:1306–1308, 1970.
Hofmann, I., and Hosein, E. A.: Effects of chronic ethanol consumption on the rate of rat liver mitochondrial protein turnover and synthesis. Biochem. Pharmacol. 27:457–463, 1978.
Iseri, O. A., Lieber, C. S., and Gottfried, L. S.: The ultrastructure of fatty liver induced by prolonged ethanol ingestion. Am. J. Pathol. 48:535–555, 1966.
Ishii, H., Joly, J.-G., and Lieber, C. S.: Effect of ethanol on the amount and enzyme activities of hepatic rough and smooth microsomal membranes. Biochem. Biophys. Acta 291:411–420,1973.
Israel, Y., and Orrego, H.: On the characteristics of alcohol-induced liver enlargement and its possible hemodynamic consequences. Pharmacol. Biochem. Behay. 18:433–437, 1983.
Israel, Y., Khanna, J. M., Orrego, H., Rachmin, G., Wahid, S., Britton, R., Macdonald, A., and Kalant, H.: Studies on metabolic tolerance to alcohol, hepatomegaly and alcoholic liver disease. Drug. Alcohol. Dep. 4:109–118, 1979.
Jauhonen, P., Baraona, E., Miyakawa, H., and Lieber, C. S.: Mechanism for selective perivenular hepatotoxicity of ethanol. Alcoholism: Clin. Exp. Res. 6:350–357, 1982.
Jauhonen, P., Baraona, E., Lieber, C. S., and Hassinen, I. E.: Dependence of ethanol-induced redox shift on hepatic oxygen tensions prevailing in vivo. Alcohol 2:163–167, 1985.
Jeejeebhoy, K. N., Phillips, M. J., Bruce-Robertson, A., Ho, J., and Sodtke, U.: The acute effect of ethanol on albumin, fibrinogen and transferrin synthesis in the rat. Biochem. J. 126:1111–1126, 1972.
Jeejeebhoy, K. N., Bruce-Robertson, A., Ho, J., and Sodtke, U.: The effect of ethanol on albumin and fibrinogen synthesis in vivo and in hepatocyte suspensions. In: Alcohol and Abnormal Protein Synthesis (M. A. Rothschild, M. Oratz, and S. S. Schrieber, eds.), New York, Pergamon, pp. 373–391, 1975.
Jennett, R. B., Rama, D. J., and Sorrell, M. F.: Effect of ethanol and its metabolites on microtubule formation. Pharmacology 21:363–368, 1980.
Jennett, R. B., Sorrell, M. F, Johnson, E. L., and Tuma, D. J.: Covalent binding of acetaldehyde to tubulin: Evidence for preferential binding to the α-chain. Arch. Biochem. Biophys. 256:10–18, 1987.
Jennett, R. B., Sorrell, M. F., Saffari-Fard, A., Ockner, J. L., and Tuma, D. J.: Preferential covalent binding of acetaldehyde to the a-chain of purified rat liver tubulin. Hepatology 9:57–62, 1989.
Kanayama, R., Takase, S., Matsuda, Y., and Takada, A.: Effect of dietary fat upon ethanol metabolism in rats. Biochem. Pharmacol. 33:3283–3287, 1984.
Kawahara, H., Matsuda, Y., and Takada, A.: Effects of ethanol on the microtubules of cultured rat hepatocytes. Alcohol Alcoholism (Suppl. 1):307–311, 1987.
Khairallah, E. K., and Mortimore, G. E.: Assessment of protein turnover in perfused rat liver. Evidence for amino acid compartmentation from differential labeling of free and t-RNA bound valine. J. Biol. Chem. 251:1375–1384, 1976.
Khawaja, J. A., and Lindholm, D. B.: Differential effect of ethanol ingestion on the protein synthetic activities of free and membrane-bound ribosomes from liver of the weanling rat. Res. Commun. Chem. Pathol. Pharmacol. 19:129–139, 1978.
Kirsch, R. E., Frith, L. O., Stead, R. H., and Saunders, S. J.: Effect of alcohol on albumin synthesis by the isolated per-fused rat liver. Am. J. Clin. Nutr. 26:1191–1194, 1973.
Klatskin, G.: The effect of ethyl alcohol on nitrogen excretion in the rat. Yale J. Biol. Med. 34:124–143, 1961.
Krebs, H. A. Freedland, A., Hems, R., and Stubbs, M.: Inhibition of hepatic gluconeogenesis by ethanol. Biochem. J. 112:117–124, 1969.
Kreisberg, R. A., Siegal, A. M., and Owen, W. C.: Alanine and gluconeogenesis in man: Effect of ethanol. J. Clin. Endocrinol. 34:876–883, 1972.
Kuriyama, K., Sze, P. Y., and Rauscher, G. E.: Effects of acute and chronic ethanol administration on ribosomal protein synthesis in mouse brain and liver. Life Sci. 10:181–189, 1971.
Lane, B. P. and Lieber, C. S.: Ultrastructural alterations in human hepatocytes following ingestion of ethanol with adequate diets. Am. J. Pathol. 49:594–603, 1966.
LeBouton, A. V: Heterogeneity of protein metabolism between liver cells as studied by radioautography. Curr. Mod. Biol. 2:111–114, 1968.
LeBouton, A. V: Relations and extent of the zone of intensified protein metabolism in the liver acinus. Curr. Mod. Biol. 3:48, 1969.
LeMarchand, Y., Singh, A., Assimacopoulos-Jeannet, F., Orci, L., Rouiller, C., and Jeanrenaud, B.: A role for the micro-tubular system in the release of very low density lipoproteins by perfused mouse livers. J. Biol. Chem. 248:6862–6870, 1973.
LeMarchand, Y., Patzelt, C., Assimacopoulos-Jeannet, F., Loten, E. G., and Jeanrenaud, B.: Evidence for a role of the micro-tubular system in the secretion of newly synthesized albumin and other proteins by the liver. J. Clin. Invest. 53:1512–1517, 1974.
Lieber, C. S., and DeCarli, L. M.: Quantitative relationship between amount of dietary fat and severity of alcoholic fatty liver. Am. J. Clin. Nutr. 23:474–478, 1970.
Lieber, C. S., and Rubin, E.: Ethanol, a hepatotoxic drug. Gastroenterology 54:642–646, 1968a.
Lieber, C. S., Rubin, E.: Alcoholic fatty liver in man on a high protein and low fat diet. Am. J. Med. 44:200–206, 1968b.
Lieber, C. S., Jones, D. P., and DeCarli, L. M.: Effects of prolonged ethanol intake: Production of fatty liver despite adequate diets. J. Clin. Invest. 44:1009–1021, 1965.
Lieber, C. S., Spritz, N., and DeCarli, L. M.: Fatty liver produced by dietary deficiencies: Its pathogenesis and potentiation by ethanol. J. Lipid Res. 10:283–287, 1969.
Lieber, C. S., Rubin, E., DeCarli, L. M., Misra, P. S., and Gang, H.: Effects of pyrazole on hepatic function and structure. Lab. Invest. 22:615–621, 1970.
Lin, G. W.-J.: Effect of ethanol feeding during pregnancy on placental transfer of alpha-amínoisobutyric acid in the rat. Life Sci. 28:595–601, 1981.
Lindros, K. O., and Aro, H.: Ethanol-induced changes in levels of metabolites related to the redox state and ketogenesis in rat liver. Ann. Med. Exp. Fenn. 47:39–42, 1969.
Madsen, N. P: Reduced serum low-density lipoprotein levels after acute ethanol administration. Biochem. Pharmacol. 18:261–262, 1969.
Mailliard, M. E., Sorrell, M. F., Volentine, G. D., and Tuma, D. J.: Impaired plasma membrane glycoprotein assembly in the liver following acute ethanol administration. Biochem. Biophys. Res. Commun. 123:951–958, 1984.
Marinari, U. M., Cottalasso, D., Gambella, G. R., Averame, M. M., Pronzato, M. A., and Nanni, G.: Effects of acute ethanol intoxication of 3H-palmitic acid transport through hepatocyte Golgi apparatus. FEBS Leu. 86:53–56, 1978.
Matsuda, Y., Baraona, E., Salaspuro, M., and Lieber, C. S.: Pathogenesis and role of microtubular alterations in alcohol-induced liver injury. Fed. Proc. 37:402, 1978.
Matsuda, Y., Baraona, E., Salaspuro, M., and Lieber, C. S.: Effects of ethanol on liver microtubules and Golgi apparatus. Possible role in altered hepatic secretion of plasma proteins. Lab. Invest. 41:455–463, 1979.
Matsuda, Y., Takada, A., Kanayama, R., and Takase, S.: Changes of hepatic microtubules and secretory proteins in human alcoholic liver disease. Alcoholism: Phmarmacol. Biochem. Behay. 18 (Suppl. 1):479–482, 1983.
Matsuda, Y., Takada, A., Sato, H., Yasuhara, M., and Takase, S.: Comparison between ballooned hepatocytes occurring in human alcoholic and non-alcoholic liver diseases. Alcoholism: Clin. Exp. Res. 9:366–370, 1985a.
Matsuda, Y., Takase, S., Takada, A., Sato, H., and Yasuhara, M.: Comparison of ballooned hepatocytes in alcoholic and nonalcoholic liver injury in rats. Alcohol 2:303–308, 1985b.
Matsuda, Y., Yasuhara, M., and Takada, A.: Subcellular differences in hepatic microtubular changes of alcoholic liver disease: morphometrical analysis. Alcohol Alcohol. Suppl. 1: 503–507, 1987.
Matthews, D. M., and Adibi, S. A.: Peptide absorption. Gastroenterology 71:151–161, 1976.
McDonald, J. T, and Margen, S.: Wine versus ethanol in human nutrition. I. Nitrogen and calorie balance. Am. J. Clin. Nutr. 29:1093–1103, 1976.
McKinnon, G., Davidson, M., De Jersey, J. D., Shanley, B., and Ward, L.: Effects of acetaldehyde on polymerization of microtubule proteins. Brain Res. 416:90–99, 1987.
Meijer, A. J., and Williamson, J. R.: Transfer of reducing equivalents across the mitochondria) membrane. I. Hydrogen transfer mechanisms involved in the reduction of pyruvate to lactate in isolated liver cells. Biochim. Biophys. Acta 333:111, 1974.
Meijer, A. J., Gimpel, J. A., Deleeuw, G. A., Tager, J. M., and Williamson, J. R.: Role of anion translocation across the mitochondria] membrane in the regulation of urea synthesis from ammonia by isolated rat hepatocytes. J. Biol. Chem. 250:7728–7738, 1975.
Moojerkea, S., and Chow, A: Impairment of glycoprotein synthesis in acute ethanol intoxication in rats. Biochim. Biophys. Acta 184:83–92, 1969.
Morgan, E. H., and Peters, T.: The biosynthesis of rat serum albumin. V. Effect of protein depletion and refeeding on albumin and transferrin synthesis. J. Biol. Chem. 246:3500, 1971a.
Morgan, E. H., and Peters, T.: Intracellular aspects of transferrin synthesis and secretion in the rat. J. Biol. Chem. 246:3508–3511, 1971b.
Morland, J.: Effects of chronic ethanol treatment on tryptophan oxygenase, tyrosine aminotransferase and general protein metabolism in the intact and perfused rat liver. Biochem. Pharmacol. 73:71–75, 1974.
Morland, J.: Incorporation of labelled amino acids into liver protein after acute ethanol administration. Biochem. Pharmacol. 24:439–442, 1975.
Morland, J., and Bessessen, A.: Inhibition of protein synthesis by ethanol in isolated rat liver parenchymal cells. Biochim. Biophys. Acta. 474:312–320, 1977.
Morland, J., and Sjetnan, A. E.: Effect of ethanol intake on the incorporation of labeled amino acids into liver protein. Biochem. Pharmacol. 25:2125–2130, 1976.
Morland, J., Bessesen, A., and Svendsen, L.: Incorporation of labeled amino acids into proteins of isolated parenchymal and non-parenchymal rat liver cells in the absence and presence of ethanol. Biochim. Biophys. Acta 561:464–474, 1979a.
Morland, J., Flengsrud, R., Prydz, H., and Svendsen, L.: Hepatic amino acid levels in rats after long-term ethanol feeding. Biochem. Pharmacol. 28:423–427, 1979b.
Morland, J., Bessesen, A., and Svendsen, L.: The role of alcohol metabolism in the effect of ethanol on protein synthesis in isolated rat hepatocytes. Alcoholism: Clin. Exp. Res. 4:313–321, 1980.
Morland, J., Rothschild, M. A., Oratz, M., Mongelli, J., Donor, D., and Schreiber, S. S.: Protein secretion in suspensions of isolated rat hepatocytes: No influence of acute ethanol administration. Gastroenterology 80:159–165, 1981.
Morland, J. Bessesen, A., Smith-Kielland, A., and Wallin, B.: Ethanol and protein metabolism in the liver. Pharmacol. Biochem. Behay. 18 (Suppl 1):252–256, 1983.
Mortimore, G. E., and Mondon, C. E.: Inhibition by insulin of valine turnover in the liver. Evidence for a general control of proteolysis. J. Biol. Chem. 245:2375–2383, 1970.
Mortimore, G. E., and Pösö, A. R.: Mechanism and control of deprivation-induced protein degradation in liver: Role of glucogenic amino acids. In: Glutamine Metabolism in Mammalian Tissues (D. Häussinger and H. Sies, eds.). Springer-Verlag, Berlin, pp. 138–157, 1984.
Moseley, R. H., and Murphy, S. M.: Effects of ethanol on amino acid transport in basolateral liver plasma membrane vesicles. Am. J. Physiol. 256:G458–465, 1989.
Munro, H. N.: Committee on Dietary Allowances. Washington, D. C., The National Research Council, National Academy of Sciences, 1978.
Murty, N., Verney, E., and Sidransky, H.: Acute effects of ethanol on membranes of the endoplasmic reticulum and on protein synthesis in the rat liver. Alcoholism: Clin. Exp. Res. 4:93–103, 1980.
Nakano, K., and Hara, H.: Measurement of the protein-synthetic activity in vivo of various tissues in rats by using [3H]puromycin. Biochem. J. 184:663–668, 1979.
Nonura, F., Pikkarainen, P H., Jauhonen, P, Arai, M., Gordon, E. R., Baraona, E., and Lieber, C. S.: The effect of ethanol administration on the metabolism of ethanol in baboons. J. Pharmacol. Exp. Ther. 227:78–83, 1983.
Okanoue, T, Ou, O., Ohta, M., Yoshida, J., Horishi, M. Y. T., Okuno, T., and Takino, T.: Effect of chronic ethanol administration on the cytoskeleton of rat hepatocytes—including morphometric analysis. Acta Hepat. Jpn. 25:210–213, 1984.
Ookhtens, M., Hobdy, K., Corvasce, M. C., Aw, T. Y., and Kaplowitz, N.: Sinusoidal efflux of glutathione in the per-fused rat liver: Evidence for carrier-mediated process. J. Clin. Invest. 75:258–265, 1985.
Oratz, M., Rothschild, M. A., and Schreiber, S. S.: Alcohol, amino acids, and albumin synthesis. II. Alcohol inhibition of albumin synthesis reversed by arginine and spermine. Gastroenterology 71:123–127, 1976.
Oratz, M., Rothschild, M. A., and Schreiber, S. S.: Alcohol, amino acids and albumin synthesis. III. Effects of ethanol, acetaldehyde and 4-methylpyrazole. Gastroenterology 74: 672–676,1978.
Oratz, M., Rothschild, M. A., Schreiber, S. S., Burks, A., Mongelii, J., and Matarese, B.: The role of the urea cycle and polyamines in albumin synthesis. Hepatology 3:567–571, 1983.
Patek, A. J., Jr., Post, J., Ratnoff, O. D., Mankin, H., and Hillman, R. W: Dietary treatment of cirrhosis of the liver. J.A.M.A. 138:543–549, 1948.
Patwardhan, R. V., Schenker, S., Henderson, G. I., Abou-Mourad, N. M., and Hoyumpa, A. M., Jr.: Short-term and long-term ethanol administration inhibits the placental uptake and transport of valine in rats. J. Lab. Clin. Med. 98:251–262, 1981.
Paul, H. S., and Adibi, S. A.: Role of ATP in regulation of branched-chain œ-keto acid dehydrogenase activity in liver and muscle mitochondria of fed, fasted, and diabetic rats. J. Biol. Chem. 257:4875–4881, 1982.
Perin, A., and Sessa, A.: In vitro effects of ethanol and acetaldehyde on tissue protein synthesis. In: The Role of Acetaldehyde in the Action of Ethanol (K. O. Lindros, and C. J. P. Eriksson, eds.), Satellite Symp. 6th Intl. Congo Pharmacol. Helsinki, The Finnish Foundation for Alcohol Studies, pp. 105–122, 1975.
Perin, A., Scalabrino, G., Sessa, A., and Arnaboldi, A.: In vitro inhibition of protein synthesis in rat liver as consequence of ethanol metabolism. Biochim. Biophys. Acta 366:101–108, 1974.
Perkoff, G. Y., Hardy, P, and Velez-Garcia, E.: Reversible acute muscular syndrome in chronic alcoholism. N. Engl. J. Med. 274:1277–1285, 1966.
Peters, T., Jr.: The biosynthesis of rat serum albumin. II. Intracellular phenomena in the secretion of newly formed albumin. J. Biol. Chem. 237:1186–1189, 1962.
Peters, T., Jr., Fleischer, B., and Fleischer, S.: The biosynthesis of rat serum albumin. IV. Apparent passage of albumin through the Golgi apparatus during secretion. J. Biol. Chem. 246: 240–244, 1971.
Piccirillo, V. J., and Chambers, J. W.: Inhibition of hepatic uptake of alpha amino-isobutyric acid by ethanol: effects of pyrazole and metabolites of ethanol. Res. Commun. Chem. Pathol. Pharmacol. 13:297–308, 1976.
Pierson, J. L, and Mitchell, M. C.: Increased hepatic afflux of glutathione after chronic ethanol feeding. Biochem. Pharmacol. 35:1533–1537, 1986.
Pignon, J.-P, Bailey, N. C., Baraona, E., and Lieber, C. S.: Fatty acid-binding protein: a major contributor to the ethanol-induced increase in liver cytosolic proteins in the rat. Hepatology 7:865–871, 1987.
Pipeleers, D. G., Pipeleers-Marichal, M. A., and Kipnis, D. M.: Physiological regulation of total tubulin and polymerized tubulin in tissues. J. Cell Biol. 74:351–357, 1977.
Plapp, F V., Updike, R. D., and Chiga, M.: Effects of ethanol on mouse liver polysomal disaggregation by dimethylnitrosamine and lasiocarpine. FEBS Lett. 18:121–123, 1971.
Porta, E. A., Koch, O. R., Gomez-Dumm, C. L. A. and Hartroft, W. S.: Effects of dietary protein on the liver of rats in experimental chronic alcoholism. J. Nutr. 94:437–446, 1968.
Pösö, A. R.: Ethanol and hepatic protein turnover. Alcohol Alcohol. Suppl. 1:83–90, 1987.
Pösö, A. R., Surmacz, C. A., and Mortimore, G. E.: Inhibition of intracellular protein degradation by ethanol in perfused rat liver. Biochem. J 242:459–464, 1987.
Potter, B. J., Chapman, R. W. G., Nunes, R. M., Sorrentino, D., and Sherlock, S.: Transferrin metabolism in alcoholic liver disease. Hepatology 5:714–721, 1985.
Preedy, V. R., and Peters, T J.: The effect of chronic ethanol ingestion on protein metabolism in Type-I and Type-II-fiberrich skeletal muscles of the rat. Biochem. J. 254:631–639, 1988.
Princen, J. M. G., Mol-Backx, G. P. B. M., and Yap, S. H.: Acute effects of ethanol intake on albumin and total protein synthesis in free and membrane-bound polyribosomes of rat liver. Biochim. Biophys. Acta. 655:119–127, 1981.
Quistorff, B., Chance, B., and Takeda, H.: Two-and three-dimensional redox heterogeneity of rat liver. Effects of anoxia and alcohol on the lobular redox pattern. In: Frontiers of Biological Energetics: Electrons to Tissues (L. T Dutton, L. S. Leigh, and A. Scarpa, eds.), New York, Academic Press, pp. 1487–1497, 1978.
Raatikainen, O. J., and Mäenpää, P H.: Ethanol and liver protein synthesis in vivo. Experientia 36:527, 1980.
Rawat, A. K.: Effect of maternal ethanol consumption on foetal and neonatal hepatic protein synthesis. Biochem. J. 160:65–3661, 1976.
Reaven, E. P., and Reaven, G. M.: Evidence that microtubules play a permissive role in hepatocyte very low density lipoprotein secretion. J. Cell Biol. 84:28–29, 1980.
Redman, C. M., and Cherian, G.: The secretory pathways of rat serum glycoproteins and albumin. Localization of newly formed proteins with the endoplasmic reticulum. J. Cell Biol. 52:231–245, 1972.
Redman, C. M., Banerjee, D., Howell, K., and Palade, G. E., Colchicine inhibition of plasma protein release from rat hepatocytes. J. Cell Biol. 66:42–59, 1975.
Redman, C. M., Banerjee, D., Manning, C., Huang, C. Y., and Green, K.: In vivo effects of colchicine on hepatic protein synthesis and on the conversion of proalbumin to serum albumin. J. Cell Biol. 77:400–416, 1978.
Reins, J. F., Heymsfield, S. B.,Wiskind, R., Casper, K., and Galambos, J. T.: Ethanol: relative fuel value and metabolic effects in vivo. Metabolism 38:125–135, 1989.
Renis, M., Giovine, A., and Bertolino, A.: Protein synthesis in mitochondrial and microsomal fractions from rat brain and liver after acute and chronic ethanol administration. Life Sci. 16:1447–1458, 1975.
Reyes, H., Levi, A. J., Gatmaitan, Z., and Arias, I. M.: Studies of Y and Z, two hepatic cytoplasmic organic anion-binding proteins: Effect of drugs, chemicals, hormones, and cholestasis. J. Clin. Invest. 50:2242–2252, 1971.
Rodrigo, C., Antezana, C., and Baraona, E.: Fat and nitrogen balance in rats with alcohol-induced fatty liver. J. Nutr. 101: 1307–1310, 1971.
Rosa, Y., and Rubin, E.: Effects of ethanol on amino acid uptake by rat liver cells. Lab. Invest. 43:366–372, 1980.
Rothschild, M. A., Oratz, M., Zimmon, D., Schreiber, S. S., Winer, I., and Van Caneghem, A.: Albumin synthesis in cirrhotic subjects with ascitis studied with carbonate-14C. J. Clin. Invest. 48:344–350, 1969.
Rothschild, M. A., Oratz, M., Mongelli, J., and Schreiber, S. S.: Alcohol induced depression of albumin synthesis: Reversal by tryptophan. J. Clin. Invest. 50:1812–1818, 1971.
Rothschild, M. A., Oratz, M., and Schreiber, S. S.: Alcohol, amino acids, and albumin synthesis. Gasteroenterology 67: 1200–1213, 1974.
Rothschild, M. A., Oratz, M., and Schreiber, S. S.: Effects of nutrition and alcohol on albumin synthesis: Alcoholism: Clin. Exp. Res. 7:28–30, 1983.
Rothschild, M. A., Oratz, M., and Schrieber, S. S.: Effects of ethanol on protein synthesis. Ann. NY Acad. Sci. 492:233–244, 1987.
Rubin, E.: Alcoholic myopathy in heart and skeletal muscle. N. Engl. J. Med. 301:28–33, 1979.
Rubin, E., and Lieber, C. S.: Early fine structural changes in human liver induced by alcohol. Gastroenterology 52:1–13, 1967a.
Rubin, E., and Lieber, C. S.: Experimental alcoholic hepatic injury in man: Ultrastructural changes. Fed. Proc. 26:1458–1467, 1967b.
Rubin, E., Hutterer, F., and Lieber, C. S.: Ethanol increases hepatic smooth endoplasmic reticulum and drug metabolizing enzymes. Science 159:1469–1470, 1968.
Rubin, E., Beattie, D. S., and Lieber, C. S.: Effects of ethanol on the biogenesis of mitochondria) functions. Lab. Invest. 23: 620–627,1970.
Rubin, E., Beattie, D. S., Toth, A., and Lieber, C. S.: Structural and functional effects of ethanol on hepatic mitochondria. Fed. Proc. 31:131–140, 1972.
Salaspuro, M. P., and Kesanienni, U. A.: Intravenous galactose elimination tests with and without ethanol loading in various clinical conditions. Scand. J. Gastroenterol. 8:681–686, 1973.
Salaspuro, M. P., Shaw, S., Jayatilleke, E., Ross, W. A., and Lieber, C. S.: Attenuation of the ethanol induced hepatic redox change after chronic alcohol consumption in baboons: Metabolic consequences in vivo and in vitro. Hepatology 1: 33–38, 1981.
Savolainen, M. J., Baraona, E., Leo, M. A., and Lieber, C. S.: Pathogenesis of the hypertriglyceridemia at early stages of alcoholic liver injury in the baboon. J. Lipid Res. 27:1073–1083, 1986.
Schapiro, R. H., Drummey, G. D., Shimizu, Y., and Isselbacher, K. J.: Studies on the pathogenesis of ethanol-induced fatty liver. II. Effect of ethanol on palmitate-1-C14 metabolism by isolated perfused rat liver. J. Clin. Invest. 43:1338–1347, 1964.
Schenker, S., Dicke, J. M., Johnson, R. F., Hays, S. E., and Henderson, G. I.: Effect of ethanol on human placental transport of model amino acids and glucose. Alcoholism: Clin. Exp. Res. 11.112–119, 1989.
Schmitt, J. W, and Elbein, A. D.: Inhibition of protein synthesis also inhibits synthesis of lipid-linked oligosaccharides. J. Biol. Chem. 254:12291–12294, 1979.
Schreiber, S. S., Oratz, M., Rothschild, M. A., Reff, F., and Evans, C.: Alcoholic cardiomyopathy. II. The inhibition of cardiac microsomal protein synthesis by acetaldehyde. J. Mol. Cell Cardiol. 6:207–213, 1974.
Seakins, A., and Robinson, D. S.: Changes associated with the production of fatty livers by white phosphorus and by ethanol in the rat. Biochem. J. 92:308–312, 1964.
Shaw, S., and Lieber, C. S.: Plasma amino acid abnormalities in the alcoholic: Respective role of alcohol, nutrition, and liver injury. Gastroenterology 74:677–682, 1978.
Sherline, E, Bodwin, C. K., and Kipnis, D. M.: A new colchicine binding assay for tubulin. Anal. Biochem. 62:400–407, 1974.
Sidransky, H., Verney, E., and Murty, C. N.: Effect of ethanol on polyribosomes and protein synthesis of transplantable hepatomas and host livers of rat. Proc. Soc. Exp. Biol. Med. 163: 335–339,1980.
Sjoblom, M., and Morland, J.: Metabolism of ethanol and acetaldehyde in parenchymal and nonparenchymal rat liver cells. Biochem. Pharmacol. 28:3417–3423, 1979.
Smith, D. J., Da Silva Daley, G., and Polch, S. A.: Ethanol consumption decreases alanine uptake by rat basolateral liver plasma membrane vesicles. Gastroenterology 98:429–436, 1990.
Smith, S. L., Jennett, R. B., Sorrell, M. F., and Tuma, D. J.: Acetaldehyde substoichiometrically inhibits bovine neurotubulin polymerization. J. Clin. Invest. 84:337–341, 1989.
Smith-Kielland, A., and Morland, J.: Changes in protein, RNA and DNA content in various rat organs after long-term intake of ethanol. Acta Pharmacol. Toxicol. 45:122–130, 1979.
Smith-Kielland, A., and Morland, J.: Reduced hepatic protein synthesis after long term ethanol treatment in fasted rats. Dependence on animal handling before measurement. Biochem. Pharmacol. 30:2377–2379, 1981.
Smith-Kielland, A., Bengtsson, G., Svendsen, L., and Morland, J.: Protein synthesis in different populations of rat hepatocytes separated according to density. J. Cell Physiol. 110:262–266, 1982.
Smith-Kielland, A., Blom, G. P., Svendsen, L., Bessesen, A., Morland, J.: A study of hepatic protein synthesis, three subcellular enzymes, and liver morphology in chronically ethanol fed rats. Acta Pharmacol. Toxicol. 53:113–120, 1983a.
Smith-Kielland, A., Svendsen, L., Bessesen, A., and Morland, J.: Effect of chronic ethanol consumption on in vivo protein synthesis in livers from female and male rats fed two different diet regimens. Alcohol Alcohol. 18:285–292, 1983b.
Sorrell, M. F., and Tuma, D. J.: Selective impairment of glycoprorein metabolism by ethanol and acetaldehyde in rat liver slices. Gastroenterology 75:200–205, 1978.
Sorrell, M. F, Tuma, D. J., Schafter, E. C., and Barak, A. J.: Role of acetaldehyde in the ethanol-induced impairment of glycoprotein metabolism in rat liver slices. Gastroenterology 73: 137–144, 1977a.
Sorrell, M. F., Tuma, D. J., and Barak, A. J.: Evidence that acetaldehyde irreversibly impairs glycoprotein metabolism in liver slices. Gastroenterology 73:1138–1141, 1977b.
Sorrell, M. E, Nauss, J. M., Donohue, T. M., and Timm, D. J.: Effects of chronic ethanol administration of hepatic glycoprotein secretion in the rat. Gastroenterology 84:580–586, 1983.
Speisky, H., MacDonald, A., Giles, G., Orrego, H., and Israel, Y.: Increased loss and decrease synthesis of hepatic glutathione after acute ethanol administration. Biochem. J. 225: 565–572,1985.
Stanko, R. T., Morse, E. L., and Adibi, S. A.: Prevention of effects of ethanol on amino acid concentrations in plasma and tissues by hepatic lipotropic factors in rats. Gastroenterology 76:132–138, 1979.
Stein, O., and Stein, Y.: Colchicine-induced inhibition of very low density lipoprotein release by rat liver in vivo. Biochim. Biophys. Acta 306:142–147, 1973.
Stein, O., Sanger, L., and Stein, Y.: Colchicine-induced inhibition of lipoprotein and protein secretion into the serum and lack of interference with secretion of biliary phospholipids and cholesterol by rat liver in vivo. J. Cell Biol. 69:90–103, 1974.
Stibler, H., Borg, S., and Allgulander, C.: Clinical significance of abnormal heterogeneity of transferrin in relation to alcohol consumption. Acta. Med. Scand. 206:275–281, 1979.
Stibler, H., Borg, S., and Allgulander, C.: Abnormal micro-heterogeneity of transferrin—a new marker of alcoholism. Subst. Alcohol Action/Misuse 1:247–252, 1980.
Stibler, H., Borg, S., and Joustra, M.: Micro anion exchange chromatography of carbohydrate-deficient transferrin in serum in relation to alcohol consumption. Alcoholism 10:535–544,1986.
Struck, D. K., Siuta, P. B., Lane, M. D., and Lennarz, W. J.: Effect of tunicamycin on the secretion of serum proteins on primary cultures of rat and chick hepatocytes. Studies on transferrin, very low density lipoprotçins and serum albumin. J. Biol. Chem. 253:5332–5337, 1978.
Swick, R. W: Measurement of protein turnover in rat liver. J. Biol. Chem. 231:751–764, 1958.
Takada, A., Matsuda, Y., and Takase, S.: Effects of dietary fat on alcohol–pyrazole hepatitis in rats: the pathogenetic role of the nonalcohol dehydrogenase pathway in alcohol-induced hepatic cell injury. Alcoholism: Clin. Exp. Res. 10:403–411, 1986.
Teschke, R., Matsuzaki, S., Ohniski, K., DeCarli, L. M., and Lieber, C. S.: Microsomal ethanol oxidizing system (MEOS): Current status of its characterization and its role. Alcoholism: Clin. Exp. Res. 1:7–15, 1977.
Tewari, S., and Noble, E. P: Ethanol and brain protein synthesis. Brain Res. 26:469–474, 1971.
Tuma, D. J., and Sorrell, M. F.: Effects of ethanol on the secretion of glycoproteins in rat liver slices. Gastroenterology 80:273–278, 1981.
Tiuna, D. J. Zetterman, R. K., and Sorrell, M. F.: Inhibition of glycoprotein secretion by ethanol and acetaldehyde in rat liver slices. Biochem. Pharmacol. 29:35–38, 1980.
Tuma, D. J., Jennett, R. B.,and Sorrell, M. E: Effect of ethanol on the synthesis and secretion of hepatic secretory glycoproteins and albumin. Hepatology 1:590–598, 1981.
’Puma, D. J., Jennett, R. B., and Sorrell, M. F.: The interaction of acetaldehyde with tubulin. Ann. NYAcad. Sci. 492:277–286, 1987.
Vazquez, J. A., Paul, H. S., and Adibi, S. A.: Regulation of leucine catabolism by caloric sources: Role of glucose and lipid in nitrogen sparing during nitrogen deprivation. J. Clin. Invest. 82:1606–1613, 1988.
Veech, R. L., Eggleston, L. V., and Krebs, H. A.: The redox state of free nicotinamide–adenine dinucleotide phosphate in cytoplasm of rat liver. Biochem. J. 115:609–619, 1969.
Veech, R. L., Guynn, R., and Veloso, D.: The time-course of the effects of ethanol on the redox and phosphorylation states of rat liver. Biochem. J. 127:387–397, 1972.
Vendemiale, G., Altomare, E., Grattagliano, I., and Albano, O.: Increased plasma levels of glutathione and malondialdehyde after acute ethanol ingestion in humans. J. Hepatol. 9:359–365,1989.
Videla, L. A., Fernandez, V, Ugarte, G., and Valenzuela, A.: Effect of acute ethanol intoxication on the content of reduced glutathione of the liver in relation to its lipoperoxidative capacity in the rat. FEBS Leu. 111:6–10, 1980.
Voci, A., Gallo, G., Balestrero, F, and Rugassa, E.: Effects of ethanol on protein metabolism in hepatocyte primary cultures from adult rat. Cell. Biol. Int. Rep. 12:647–660, 1988.
Volentine, G. D., Tuma, D. J., and Sorrell, M. F: Acute effects of ethanol on hepatic glycoprotein secretion in the rat in vivo. Gastroenterology 86:225–229, 1984.
Volentine, G. D., Zirma, D. J., and Sorrell, M. F.: Subcellular location of secretory proteins retained in the liver during the ethanol-induced inhibition of hepatic protein secretion in the rat. Gastroenterology 90:158–165, 1986.
Wahlander, A., Soboll, A., and Sies, H.: Hepatic mitochondrial and cytosolic glutathione content and the subcellular distribution of GSH-S transferases. FEBS Lett. 97:138–140, 1975.
Wallin, B., Morland, J., and Fikke, A.-M.: Combined effects of ethanol and pH-change on protein synthesis in isolated rat hepatocytes. Acta Pharmacol. Toxicol. 49:134–140, 1981.
Wallin, B., Bessesen, A., Fikke, A. M., Aabakke, J., and Morland, J.: No effect of acute ethanol administration on hepatic protein synthesis and export in the rat in vivo. Alcoholism 8:191–195,1984.
Wallyn, C. S., Vidrich, A., Airhart, J., and Khairallah, E. A.: Analysis of the specific radioactivity of valine isolated from amino-acyl-transfer ribonucleic acid of rat liver. Biochem. J. 140:545–548, 1974.
Ward, L. C., Carrington, L. E., and Daly, R.: Ethanol and leucine oxidation—I. Leucine oxidation by the rat in vivo. Int. J. Biochem. 17:187–193, 1985a.
Ward, L. C., Ramm, G. A., Mason, S., and Daly, R.: Ethanol and leucine oxidation—II. Leucine oxidation by rat tissue in vitro. Int. J. Biochem. 17:195–201, 1985b.
Wasterlain, C. G., Dwyer, B. E., Fando, J., Salinas-Fando, M., and Lieber, C. S.: The effects of ethanol and acetaldehyde on brain proteins and DNA synthesis are independent of nutritional effects. In: Alcohol and Protein-Synthesis: Ethanol, Nucleic Acid, and Protein Synthesis in the Brain and Other Organs, Washington, D.C., NIAAA, DHHS Public No. (ADM) 83–1198, pp. 199–218, 1983.
Weesner, R. E., Mendenhall, C. L., Morgan, D. D., Kessler, V, Kromme, C.: Serum-fetoprotein: Changes associated with acute and chronic ethanol ingestion in the resting and regenerating liver. J. Lab. Clin. Med. 95:725–736, 1980.
Weigand, K., Otto, I., and Schopf, R.: Ficoll density separation of enzymatically isolated rat liver cells. Acta Hepato-Gastroenterol. 21:245–253, 1974.
Weigand, K., Richter, E., and Esperer, H.-D.: Biochemical studies of isolated rat hepatocytes from normal and phenobarbital-treated liver as obtained by rate zonal centrifugation. Acta Hepato-Gastroenterol. 24:170–174, 1977.
Wool, I. G., and Kurihara, K.: Determination of the number of active muscle ribosomes: Effects of diabetes and insulin. Proc. NY Acad. Sci. 58:2401–2407, 1967.
Wooles, W. R.: Depressed fatty acid oxidation as a factor in the etiology of acute ethanol-induced fatty liver. Life Sci. 5:267–276,1966.
Wu, J. M.: Control of protein synthesis in rabbit reticulocytes. Inhibition of polypeptide synthesis by ethanol. J. Biol. Chem. 256:4164–4167, 1981.
Xu, D. S., Jennett, R. B., Smith, S. L., Sorrell, M. F, and Tuma, D. J.: Covalent interactions of acetaldehyde with the actin/ microfilament system. Alcohol Alcohol. 24:281–289, 1989.
Yasuhara, M., Matsuda, Y., and Takada, A: Degradation of acetaldehyde produced by the nonatcohol dehydrogenase pathway. Alcoholism: Clin. Exp. Res. 10:545–549, 1986.
Zern, M. A., Chakraborty, P R., Ruiz-Opazo, N., Yap, S. H., and Shafritz, D. A.: Development and use of a rat albumin cDNA clone to evaluate the effect of chronic ethanol administration on hepatic protein synthesis. Hepatology 3:317–322, 1983.
Zern, M. A., Leo, M. A., Giambrone, M.-A., and Lieber, C. S.: Increased type I procollagen mRNA levels and in vitro protein synthesis in the baboon model of chronic alcoholic liver disease. Gastroenterology 89:1123–1131, 1985.
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Adibi, S.A., Baraona, E., Lieber, C.S. (1992). Effects of Ethanol on Amino Acid and Protein Metabolism. In: Medical and Nutritional Complications of Alcoholism. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3320-7_5
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