Expression and Regulation of the Maize Spm Transposable Element

  • N. V. Fedoroff
  • P. Masson
  • J. A. Banks
Part of the NATO ASI Series book series (NSSA, volume 212)


There is both genetic and molecular evidence that the maize Suppressor-imitator (Spm) element encodes gene products necessary for transposition and autoregulation (reviewed in Fedoroff, 1983, 1989, Gierl et al., 1989). Identification of the element-encoded gene products that participate in transposition and regulation has been hindered by the pleiotropy of spontaneous mutations in the element and a poor understanding of how the element is regulated. Here we review recent progress in our laboratory in the identification and characterization of element-encoded transcripts, as well as in understanding both positive and negative regulation of Spm activity.


Transposable Element Transgenic Tobacco Frameshift Mutation Splice Donor Site Large Transcript 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Banks, J., Masson, P., and Fedoroff, N., 1988, Molecular mechanisms in the developmental regulation of the maize Suppressor-mutator transposable element, Genes Dev. 2:1364.PubMedCrossRefGoogle Scholar
  2. Banks, J. A., and Fedoroff, N., 1989, Patterns of developmental and heritable change in methylation of the Suppressor-mutator transposable element, Develop. Genet. 10:425.CrossRefGoogle Scholar
  3. Fedoroff, N. V., 1983, Controlling elements in maize, in “Mobile Genetic Elements”, J. Shapiro, ed., Academic Press, New York, pp. 1–63.Google Scholar
  4. Fedoroff, N. V., and Banks, J. A., 1988, Is the Suppressor-mutator element controlled by a basic developmental regulatory mechanism?, Genetics 120:559.Google Scholar
  5. Fedoroff, N., 1989a, Maize transposable elements, in “Mobile DNA”, M. Howe, and D. Berg, eds., American Society for Microbiology, Washington, pp. 375–411.Google Scholar
  6. Fedoroff, N. V., 1989b, The heritable activation of cryptic Suppressormutator elements by an active element, Genetics 121:591.PubMedGoogle Scholar
  7. Gierl, A., Lütticke, S., and Saedler, H., 1988, TnpA product encoded by the transposable element En-1 of Zea mays is a DNA binding protein, EMBO J. 7:4045.PubMedGoogle Scholar
  8. Gierl, A., Saedler, H., and Peterson, P. A., 1989, Maize transposable elements, Annu. Rev. Genet. 23:71.PubMedCrossRefGoogle Scholar
  9. Kozak, M., 1986, Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes, Cell 44:283.PubMedCrossRefGoogle Scholar
  10. Masson, P., Surosky, R., Kingsbury, J., and Fedoroff, N. V., 1987, Genetic and molecular analysis of the Spm-dependent a-m2 alleles of the maize a locus, Genetics 177:117.Google Scholar
  11. Masson, P., and Fedoroff, N., 1989, Mobility of the maize Suppressormutator element in transgenic tobacco cells, Proc. Natl. Acad. Sci. USA 86:2219.PubMedCrossRefGoogle Scholar
  12. Masson, P., Rutherford, G., Banks, J. A., and Fedoroff, J. A. (1989). Essential large transcripts of the maize Spm element are generated by alternative splicing. Cell, 58, 755–765.PubMedCrossRefGoogle Scholar
  13. McClintock, B., 1958, The Suppressor-imitator system of control of gene action, Carnegie Inst. Wash. Year Book 57:415.Google Scholar
  14. McClintock, B., 1959, Genetic and cytological studies of maize, Carnegie Inst. Wash. Year Book 58:452.Google Scholar
  15. McClintock, B., 1962, Topographical relations between elements of control systems in maize, Carnegie Inst. Wash. Year Book 61:448.Google Scholar
  16. Mueller, P. P., and Hinnebusch, A. G., 1986, Multiple upstream AUG codons mediate translational control of GCN4, Cell 45:201.PubMedCrossRefGoogle Scholar
  17. Pereira, A., Cuypers, H., Gierl, A., Schwarz-Sommer, Z., and Saedler, H., 1986, Molecular analysis of the En/Spm transposable element system of Zea mays, EMBO J. 5:835.PubMedGoogle Scholar
  18. Peterson, P. A., 1966, Phase variation of regulatory elements in maize, Genetics 54:249.PubMedGoogle Scholar
  19. Saiki, R. K., Scharf, S., Faloona, F., Mussil, K. B., Horn, G. T., Erlich, H. A., and Arnheim, N., 1985, Enzymatic amplification of ß-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia, Science 230:1350.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • N. V. Fedoroff
    • 1
  • P. Masson
    • 1
    • 3
  • J. A. Banks
    • 2
  1. 1.Department of EmbryologyCarnegie Institution of WashingtonBaltimoreUSA
  2. 2.Biology DepartmentMcGill UniversityMontrealCanada
  3. 3.Faculty of Agronomy and Station for Plant BreedingGemblouxBelgium

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