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Thrombin pp 315-350 | Cite as

Functional Domains in Thrombin Outside the Catalytic Site

Cellular Interactions
  • Rachel Bar-Shavit
  • Miriam Benezra
  • Valerie Sabbah
  • Elisabetta Dejana
  • Israel Vlodavsky
  • George D. Wilner

Abstract

Thrombin is known to be sequestered within the matrix of the fibrin gel, where it may remain active and intact for extended periods of time (Wilner et al., 1981; Mann, 1987). Since deposition of fibrin accompanies wound healing, thrombin may participate in modulation of this process. Inflammation is a localized protective response elicited by injury or destruction of tissues, which serves to destroy both the injurious agent and the injured tissue. Most forms of acute and chronic inflammation are amplified and propagated as a result of the recruitment of humoral and cellular components of the immune system. Immunologically mediated elimination of foreign material proceeds through a series of integrated steps. The actual destruction of antigens by immune mechanisms is mediated by cells with phagocytic capability. Such cells may migrate freely or may exist at fixed sites as components of the mononuclear phagocyte system. Since inflammation in its early stages is characterized by an influx of inflammatory cells, we wondered whether thrombin might participate in recruitment of such cells.

Keywords

Heparan Sulfate Multifunctional Protein J774 Cell Human Peripheral Blood Monocyte Thrombin Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations used

ATIII

anti-thrombin III

bFGF

basic fibroblast growth factor

BMDMs

bone-marrow-derived macrophages

DIP-α-thrombin

diisopropylfluorophosphate α-thrombin

EC

endothelial cell

ECM

extracellular matrix

FMLP

f-Met-Leu-Phe-OH

MeSO2

methylsulfonyl fluoride

PPACK

d-phenylalanyl-l-prolyl-l-arginyl-chloromethyl ketone

SMC

smooth muscle cell

TLCK

N-α-tosyl-l-lysine chloromethyl ketone

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Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Rachel Bar-Shavit
    • 1
  • Miriam Benezra
    • 1
  • Valerie Sabbah
    • 1
  • Elisabetta Dejana
    • 2
  • Israel Vlodavsky
    • 1
  • George D. Wilner
    • 3
  1. 1.Department of OncologyHadassah University HospitalJerusalemIsrael
  2. 2.Instituto di Ricerche FarmacologicheMilanoItaly
  3. 3.Department of Medicine, Division of HematologyThe Albany Medical CollegeAlbanyUSA

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