Screening for Antifungal Activity

  • John F. Ryley
  • Keith Barrett-Bee

Abstract

The purpose of antifungal screening is not to define the therapeutic properties of an individual compound, but rather to indicate which of a large number of compounds or samples are worthy of further study. The purpose is simply to provide a YES/NO answer to the question, “Is there sufficient interaction between this sample and fungi to warrant further investigation?” The attempt is to eliminate those samples which are of no definite interest, rather than to be dogmatic about samples which may be of interest. One wishes to reduce the size of a batch of samples considerably, so that those which remain can be subject to more careful study. Screening is not testing a handful of compounds for activity; it is essentially playing the numbers game. If compounds identified as interesting by the screen subsequently prove to be of no value (false positives), that does not matter; it is important that the screen does not reject compounds which really are of interest (false negatives). Once the preliminary process of screening is complete, the few samples which have been identified as possibly of interest can be evaluated in more comprehensive tests to determine their real potential and limitations. It is important to recognize the distinction between screening and evaluation. Screening is the detection of potentially interesting activity; evaluation is the quantitation and characterization of that activity.

Keywords

Fluconazole Mannose Itraconazole Chitinase Squalene 

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Copyright information

© Springer Science+Business Media Dordrecht 1992

Authors and Affiliations

  • John F. Ryley
  • Keith Barrett-Bee

There are no affiliations available

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