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Cellular Fatty Acid-Binding Proteins II pp 175–184Cite as

Release of fatty acid-binding protein and long chain fatty acids from isolated rat heart after ischemia and subsequent calcium paradox

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Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 10))

Abstract

To obtain insight into the relation between the release of heart-type fatty acid-binding protein (H-FABPc) and of long-chain fatty acids (FA) from injured cardiac tissue, rat hearts were Langendorff perfused according to the following scheme: 30 min normoxia, 60 min ischemia, 30 min reperfusion, 10 min Ca2+ free perfusion and finally 10 min Ca2+ repletion. During this protocol right ventricular (Q rv) and interstitial effluent samples (Q) were collected at regular intervals. During reperfusion a total of 0.8 ± 0.1 nmol H-FABPc but no FA were detected in the effluents. However, during Ca2+ readmission, 45 ± 4 nmol H-FABPc (80–90% of total tissue content) was released with an initial (first 3 min) simultaneous release of FA (FA/H-FABP,. ratio 0.90 ± 0.07 mol/mol). Thereafter, FA release continued at 10– 15 nmol per min mainly in Q rv while the rate of H-FABPc release decreased. During Ca2+ repletion, tissue FA content raised rapidly from 168 ± 20 to 1918 ± 107 nmol/g dry weight. These findings suggest that after severe cardiac damage initially FA is released bound to H-FABPC, whereas further FA release occurs in a non-protein bound manner.

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Authors and Affiliations

  1. Department of Physiology, Cardiovascular Research Institute Maastricht (CARIM), University of Limburg, P.O. Box 616, 6200 MD, Maastricht, The Netherlands

    Michaël M. Vork, Jan F. C. Glatz & Ger J. van der Vusse

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  1. Michaël M. Vork
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  2. Jan F. C. Glatz
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  3. Ger J. van der Vusse
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Editors and Affiliations

  1. Department of Physiology, University of Limburg, Maastricht, The Netherlands

    Jan F. C. Glatz  & Ger J. van der Vusse  & 

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© 1993 Springer Science+Business Media Dordrecht

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Vork, M.M., Glatz, J.F.C., van der Vusse, G.J. (1993). Release of fatty acid-binding protein and long chain fatty acids from isolated rat heart after ischemia and subsequent calcium paradox. In: Glatz, J.F.C., van der Vusse, G.J. (eds) Cellular Fatty Acid-Binding Proteins II. Developments in Molecular and Cellular Biochemistry, vol 10. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3096-1_23

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  • DOI: https://doi.org/10.1007/978-1-4615-3096-1_23

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6353-8

  • Online ISBN: 978-1-4615-3096-1

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