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Radial Spread of Aequorin Ca2+ Signal in Single Frog Skeletal Muscle Fibers

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Cellular Function and Metabolism

Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 9))

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Abstract

The Ca2+-sensitive photoprotein aequorin was injected into single frog skeletal muscle fibers, and the intracellular aequorin light intensity during muscle activation with different maneuvers was mapped with digital imaging microscopy. During 50 Hz electrical activation (tetanus), the aequorin light intensity from different locations in the muscle fiber rose with very similar time course. Caffeine (10 mM) application, on the other hand, caused aequorin light signals to show significantly different time courses, with an earlier increase in Ca2+ concentration near the surface of the fiber than near the core. The non-uniform rise of intracellular Ca2+ concentration with caffeine treatment is consistent with the slow inward diffusion of caffeine and subsequent Ca2+ release from sarcoplasmic reticulum. (Mol Cell Biochem 119: 59–66, 1993)

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Authors and Affiliations

  1. Department of Physiology, The Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo, 105, Japan

    Masato Konishi & Satoshi Kurihara

Authors
  1. Masato Konishi
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  2. Satoshi Kurihara
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Editors and Affiliations

  1. Third Department of Internal Medicine Faculty of Medicine, University of Tokyo, Bunkyo-ku, Hongo 7-3-1, Tokyo 113, Japan

    Yoshio Yazaki (Professor of Medicine) (Professor of Medicine)

  2. Department of Internal Medicine, Jikei University School of Medicine, Katsushika-ku, Aoto 6-41-2, Tokyo 125, Japan

    Seibu Mochizuki (Professor of Medicine) (Professor of Medicine)

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© 1993 Springer Science+Business Media Dordrecht

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Konishi, M., Kurihara, S. (1993). Radial Spread of Aequorin Ca2+ Signal in Single Frog Skeletal Muscle Fibers. In: Yazaki, Y., Mochizuki, S. (eds) Cellular Function and Metabolism. Developments in Molecular and Cellular Biochemistry, vol 9. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3078-7_9

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  • DOI: https://doi.org/10.1007/978-1-4615-3078-7_9

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6344-6

  • Online ISBN: 978-1-4615-3078-7

  • eBook Packages: Springer Book Archive

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