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Purification and Properties of Pyridoxal Kinase from Bovine Brain

  • Tae Hirakawa-Sakurai
  • Kiyoshi Ohkawa
  • Makoto Matsuda
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 9)

Abstract

A 27, 000-fold purification of pyridoxal kinase from bovine brain tissue has been achieved by a combination of ammonium sulfate fractionation, DEAE-cellulose chromatography, hydroxyapatite chromatography, Sephadex G-150 gel filtration, Blue Sepharose CL-6B chromatography, and Phenyl-Superose chromatography. The final chromatography step yields a homogeneous preparation of high specific activity (2105 nmol/min/mg protein). The molecular mass of the native enzyme was estimated to be approximately 80, 000 on gel filtration. The subunit 39, 500. This indicates that pyridoxal kinase is a dimeric enzyme. (Mol Cell Biochem 119: 203–207, 1993)

Key words

pyridoxal kinase bovine brain vitamin B6 

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Copyright information

© Springer Science+Business Media Dordrecht 1993

Authors and Affiliations

  • Tae Hirakawa-Sakurai
    • 1
  • Kiyoshi Ohkawa
    • 1
  • Makoto Matsuda
    • 1
  1. 1.Dept. BiochemJikei UnivMinato-KuTokyoJapan

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