Expression and Refolding of Functional Human Butyrylcholinesterase from E. Coli

  • Patrick Masson
  • Steve Adkins
  • Philippe Pham-Trong
  • Oksana Lockridge


Human butyrylcholinesterase (BuChE; EC. is directed by a single gene 1–3. The BCHE gene is polymorphic (cf. other papers in this book) and some of its alleles produce plasma BuChE allozymes with abnormal kinetic properties. Though no physiological function has yet been assigned to this enzyme, it is of pharmacological importance since plasma BuChE is involved in the hydrolysis of various ester-containing drugs 4. Moreover this enzyme has proven to be an efficient scavenger to protect against organophosphate poisons 5


Organophosphate Poison Inclusion Body Protein Butyrylthiocholine Iodide Inclusion Body Fraction Human Serum Cholinesterase 
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Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Patrick Masson
    • 1
  • Steve Adkins
    • 2
  • Philippe Pham-Trong
    • 1
  • Oksana Lockridge
    • 3
  1. 1.Centre de Recherches du Service de Santé des ArméesLa Tronche CedexFrance
  2. 2.Toxicology DepartmentUniversity of Michigan Medical SchoolAnn ArborUSA
  3. 3.University of Nebraska Medical CenterEppley InstituteOmahaUSA

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