Testicular Gene Amplification and Impaired BCHE Transcription Induced in Transgenic Mice by the Human BCHE Coding Sequence
Multiple findings implicate acetylcholine with sperm functioning 1,2 and acetyl-and butyrylcholinesterase activities (ACHE, BCHE) were observed in mammalian sperm cells and during oocyte development 1–3. In vivo amplification of the human BCHE gene was first found in a father and son exposed to cholinesterase inhibitors 4, but it remained unclear whether the amplified DNA was transmitted as such from father to son or whether the amplification phenomenon re-occurred in germ cells, particularly during male meiosis or sperm differentiation.
KeywordsTransgenic Mouse Cholinesterase Inhibitor Sperm Differentiation Butyrylcholinesterase Activity Endogenous Ache
Unable to display preview. Download preview PDF.
- 1.B.V. Rama Sastry, V.E. Janson and A.K. Chaturvedi. Inhibition of human sperm motility by inhibitors of choline acetyltransferase. J. Pharmacol. Exp. Ther. 216: 378–384 (1981).Google Scholar
- 3.G. Malinger, H. Zakut and H. Soreq. Cholinoceptive properties of human primordial, pre-antral and mature oocytes: In-situ hyridization and biochemical evidence for expression of cholinesterase genes. J. Mol. Neuroscience 1: 77–84 (1989).Google Scholar
- 10.H. Zakul, Y. Lapidot-Lifson, R. Beeri, et al. In-vivo gene amplification in non cancerous cells: cholinesterase genes and oncogenes amplify in thrombocytopenia associated with Lupus erythematosus. Mutation Research, in press. (1992).Google Scholar
- 12.R. Beeri, A. Gnatt, Y. Lapidot-Lifson, et al. Gene amplification and its impaired transmission studied in transgenic mice canying human butyrylcholinesterase cDNA. submitted (1992).Google Scholar
- 13.E. Kim, S.H. Waters, L.E. Hake, et al. Identification and developmental expression of a smooth-muscle gamma-actin in postmeiotic male germ cells of mice. Mol. Cell Biol. 1875 1881 (1989).Google Scholar