Effects of Pseudomonas aeruginosa on Immune Functions

  • Mario Campa
  • Paola Marelli
  • Antonella Lupetti
Part of the Infectious Agents and Pathogenesis book series (IAPA)


The ubiquitous distribution of Pseudomonas aeruginosa contrasts with its limited colonizing potential for healthy individuals. Despite the fact that P. aeruginosa is able to produce a large variety of virulence factors, this microorganism undoubtedly remains an opportunistic pathogen that can infect and eventually cause disease only in patients with defects in local or systemic immunity.


Cystic Fibrosis Pseudomonas Aeruginosa Cystic Fibrosis Patient Alkaline Protease Purify Protein Derivative 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Pollack, M., 1990, Pseudomonas aeruginosa, in: Principles and Practice of Infectious Diseases (G.L. Mandell, L.G. Douglas, and J.E. Bennett, eds.), Churchill Livingston, New York, pp. 1673–1691.Google Scholar
  2. 2.
    Schoental, R., 1941, The nature of the antibacterial agents present in Pseudomonas pyocyanea cultures, Br. J. Exp. Pathol. 22:137–147.Google Scholar
  3. 3.
    Ingram, J.M., and Blackwood, A. C., 1970, Microbial production of phenazines, Adv. Appl. Microbiol. 13:267–282.CrossRefGoogle Scholar
  4. 4.
    Leisinger, T., and Margraff, R., 1979, Secondary metabolites of the fluorescent pseudo-monads, Microbiol. Rev. 43:422–442.PubMedGoogle Scholar
  5. 5.
    Wilson, R., Pitt, T., Taylor, G., Watson, D., Mac Dermot, J., Sykes, D., Roberts, D., and Cole, P, 1987, Pyocyanin and 1-hydroxyphenazine produced by Pseudomonas aeruginosa inhibit the beating of human respiratory cilia in vitro,J. Clin. Invest. 79:221–229.PubMedCrossRefGoogle Scholar
  6. 6.
    Munro, N., Barker, A., Rutman, A., Taylor, G., Watson, D., Mac Donald-Gibson, W., Towart, R., Taylor, W., Wilson, R., and Cole, P., 1989, Effect of pyocyanin and 1-hydroxyphenazine on in vivo tracheal mucus velocity, J. Appl. Physiol. 67:316–323.PubMedGoogle Scholar
  7. 7.
    Hingley, S. T., Hastie, A. T., Kueppers, E., Higgins, M. L., Weinbaum, G., and Shryock, T., 1986, Effect of ciliostatic factors from Pseudomonas aeruginosa on rabbit respiratory cilia, Infect. Immun. 51:254–262.PubMedGoogle Scholar
  8. 8.
    Hauser, G., and Karnovsky, M. L., 1954, Studies on the production of glycolipide by Pseudomonas aeruginosa,J. Bacteriol. 68:645–654.PubMedGoogle Scholar
  9. 9.
    Liu, P. V., 1974, Extracellular toxins of Pseudomonas aeruginosa, Infect. Dis. 130:S94–S99.CrossRefGoogle Scholar
  10. 10.
    Knight, M., Hartman, P. E., Hartman, Z., and Young, V. M., 1979, A new method of preparation of pyocyanine and demonstration of an unusual bacterial sensitivity, Anal. Biochem. 95:19–23.PubMedCrossRefGoogle Scholar
  11. 11.
    Wooley, M. A., and Mc Longhlin, A. S., 1982, The regulation of pyocyanine production in Pseudomonas aeruginosa, Eur. J. Appl. Microbiol. Biotechnol. 15:161–166.CrossRefGoogle Scholar
  12. 12.
    Woods, D. E., Straus, D. C., Johanson, W. G., Jr, Berry, V. K., and Bass, J. A., 1980, Role of pili in adherence of Pseudomonas aeruginosa to mammalian buccal epithelial cells, Infect. Immun. 29:1146–1151.PubMedGoogle Scholar
  13. 13.
    Ramphal, R., Sadoff, J. C., Pyle, M., and Silipigni, J. D., 1984, Role of pili in adherence of Pseudomonas aeruginosa to injured tracheal epithelium, Infect. Immun. 44:38–40.PubMedGoogle Scholar
  14. 14.
    Baker, N., and Marcus, H., 1980, Adherence of clinical isolates of Pseudomonas aeruginosa to hamster tracheal epithelium in vitro,Curr. Microbiol. 7:35–40.CrossRefGoogle Scholar
  15. 15.
    Vishwanath, S., and Ramphal, R., 1985, Tracheobronchial mucins receptor for Pseudomonas aeruginosa: Predominance of amino sugars in binding sites, Infect. Immun. 48:331–335.PubMedGoogle Scholar
  16. 16.
    Linker, A., and Jones, R. S., 1966, A new polysaccharide resembling alginic acid isolated from pseudomonads, J. Biol. Chem. 241:3845–3851.PubMedGoogle Scholar
  17. 17.
    Doig, P., Smith, N. R., Todd, T., and Irvin, R. T., 1987, Characterization of the binding of Pseudomonas aeruginosa alginate to human epithelial cells, Infect. Immun. 55:1517–1522.PubMedGoogle Scholar
  18. 18.
    Baker, N. R., Mino F. V., Deal, C., Shahrabadi, M.S., Simpson, D. A., and Woods, D. E., 1991, Pseudomonas aeruginosa exoenzyme-S is an adhesin, Infect. Immun. 59:2859–2863.Google Scholar
  19. 19.
    Baker, N., and Svanborg-Eden, C., 1989, Role of alginate in the adherence of Pseudomonas aeruginosa,Antibiot. Chemother. 42:72–79.PubMedGoogle Scholar
  20. 20.
    Woods, D. E., Straus, D. C., Johanson, W. G., Jr., and Bass J. A., 1981, Role of fibronectin in the prevention of adherence of Pseudomonas aeruginosa to buccal cells, J. Infect. Dis. 143: 784–789.PubMedCrossRefGoogle Scholar
  21. 21.
    Suter, S., Shaad, U. B., Morgenthaler, J. J., Chevallier, I., and Schnebli, H. P., 1988, Fibronectin-cleaving activity in bronchial secretions of patients with cystic fibrosis, J. Infect. Dis. 158:89–100.PubMedCrossRefGoogle Scholar
  22. 22.
    Smallman, L. A., Hill, S. L., and Stockley, R. A., 1984, Reduction of ciliary beat frequency in vitro by sputum from patients with bronchiectasis: A serine proteinase effect, Thorax 39: 663–667.PubMedCrossRefGoogle Scholar
  23. 23.
    Ramphal, R., Small, P. A., Shands, J. W., Jr., Fischlsweiger, W., and Small, P. A., Jr., 1980, Adherence of Pseudomonas aeruginosa to tracheal cells injured by influenza infection or by endotracheal intubation, Infect. Immun. 27:614–619.PubMedGoogle Scholar
  24. 24.
    Ramphal, R., and Pyle, M., 1983, Adhesion of mucoid and non-mucoid Pseudomonas aeruginosa to acid-injured tracheal epithelium, Infect. Immun. 41:345–351.PubMedGoogle Scholar
  25. 25.
    Weinberg, E. D., 1978, Iron and Infection, Microbiol. Rev. 42:45–66.PubMedGoogle Scholar
  26. 26.
    Payne, S. M., and Finkelstein, R. A., 1978, The critical role of iron in host-bacterial interactions, J. Clin. Invest. 61:1428–1440.PubMedCrossRefGoogle Scholar
  27. 27.
    Bullen, J. J., 1981, The significance of iron in infection, Rev. Infect. Dis. 3:1127–1137.PubMedCrossRefGoogle Scholar
  28. 28.
    Liu, P. V., and Shokrani, E., 1978, Biological activities of pyochelins: Iron-chelating agents of Pseudomonas aeruginosa, Infect. Immun. 22:878–890.PubMedGoogle Scholar
  29. 29.
    Cox, C. D., Rinehart, K. L., Moore, M. L., and Cook, J. C., 1981, Pyochelin: Novel structure of an iron-chelating growth promoter for Pseudomonas aeruginosa, Proc. Natl. Acad. Sci USA 78:4256–4260.PubMedCrossRefGoogle Scholar
  30. 30.
    Meyer, J. M., Mock, M., and Abdullah, M. A., 1970, Effect of iron on the protein composition of the outer membrane of fluorescent pseudomonads, FEMS Microbiol. Lett. 5:395–398.CrossRefGoogle Scholar
  31. 31.
    Ohkawa, I. Shiga, S., and Kageyama, M., 1980, Effect of iron concentration in the growth medium on the sensitivity of Pseudomonas aeruginosa to pyocin S2, J. Biochem. 87:323–331.PubMedGoogle Scholar
  32. 32.
    Sokol, P. A., and Woods, D. E., 1983, Demonstration of an iron-siderophore-binding protein in the outer membrane of Pseudomonas aeruginosa,Infect. Immun. 40:665–669.PubMedGoogle Scholar
  33. 33.
    Sokol, P. A., 1984, Production of the ferripyochelin outer membrane receptor by Pseudomonas species, FEMS Microbiol. Lett. 23:313–317.CrossRefGoogle Scholar
  34. 34.
    Cox, C. D., 1985, Iron transport and serum resistance in Pseudomonas aeruginosa,Antibiot. Chemother. 36:1–12.PubMedGoogle Scholar
  35. 35.
    Costerton, J. W., Brown, M. R. W, and Sturgess, J. M., 1979, The cell envelope: Its role in infection, in: Pseudomonas aeruginosa: Clinical Manifestations of Infection and Current Therapy (R. G. Doggett, ed.), Academic Press, London, pp. 41–62.Google Scholar
  36. 36.
    Ulmer, A. J., Pryjma, J., Tarnok, Z., Ernst, M., and Flad, H. D., 1990, Inhibitory and stimulatory effects of Pseudomonas aeruginosa pyocyanine on human T and B lymphocytes and human monocytes, Infect. Immun. 58:808–815.PubMedGoogle Scholar
  37. 37.
    Misfeldt, M. L., Legaard, P. K., Howell, S. E., Fornella, M. H., and Legrand, R. D., 1990, Induction of interluekin-1 from murine peritoneal macrophages by Pseudomonas aeruginosa exotoxin A, Infect. Immun. 58:978–982.PubMedGoogle Scholar
  38. 38.
    Bergmann, U., Scheffer, J., Köller, M., Schönfeld, W, Erbs, G., Müller, E E., and König, W, 1989, Induction of inflammatory mediators (histamine and leukotrienes) from rat peritoneal mast cells and human granulocytes by Pseudomonas aeruginosa strains from burn patients, Infect. Immun. 57:2187–2195.PubMedGoogle Scholar
  39. 39.
    Meyers, D. J., and Berk, R. S., 1990, Characterization of phospholipase C from Pseudomonas aeruginosa as a potent inflammatory agent, Infect. Immun. 58:659–666.PubMedGoogle Scholar
  40. 40.
    Holder, I. A., and Neely A. N., 1989, Pseudomonas elastase acts as a virulence factor in burned hosts by Hageman factor-dependent activation of the host kinin cascade, Infect. Immun. 57:3345–3348.Google Scholar
  41. 41.
    Yamamoto, T, Shibuya, Y., Nishino, N., Okabe, H., and Kambara, T., 1990, Activation of human Hageman factor by Pseudomonas aeruginosa elastase in the presence or absence of negatively charged substances in vitro, Biochim. Biophys. Acta 1038:231–239.PubMedCrossRefGoogle Scholar
  42. 42.
    Pitt, T L., 1989, Lipopolysaccharide and virulence of Pseudomonas aeruginosa,Antibiot. Chemother. 42:1–7.PubMedGoogle Scholar
  43. 43.
    Kharazmi, A., Schiotz, P. O., Hoiby, N., Baeck, L., and Döring, G., 1986, Demonstration of neutrophil chemotactic activity in the sputum of cystic fibrosis patients with Pseudomonas aeruginosa infection, Eux j Clin. Invest. 16:143–148.CrossRefGoogle Scholar
  44. 44.
    Schiotz, P. O., Sorensen, H., and H•iby, N., 1979, Activated complement in the sputum from patients with cystic fibrosis, Acta Pathol. Microbiol. Scand. (C) 87:1–5.Google Scholar
  45. 45.
    Peterson, P. K., Verhoef, J., Schmeling, D., and Quie, P. G., 1977, Kinetics of phagocytosis and bacterial killing by human polymorphonuclear leukocytes and monocytes, J. Infect. Dis. 136:502–509.PubMedCrossRefGoogle Scholar
  46. 46.
    Kharazmi, A., Döring, G., Høiby, N., and Valerius, N.H.., 1984, Interaction of Pseudomonas aeruginosa alkaline protease and elastase with human polymorphonuclear leukocytes in vitro, Infect. Immun. 43:161–165.PubMedGoogle Scholar
  47. 47.
    Döring, G., Obernesser, H. J., and Botzenhart, K., 1981, Extracellular toxins of Pseudomonas aeruginosa. II. Effect of two proteases on human immunoglobulins IgG, IgA and secretory IgA, Zentralbi. Bakteriol. Mikrobiol. Hyg. (A) 249:89–98.Google Scholar
  48. 48.
    Fick, R. B., Jr., Baltimore, R. S., Squier, S. U., and Reynolds, H. Y., 1985, IgG proteolytic activity of Pseudomonas aeruginosa in cystic fibrosis, 1985, J. Infect. Dis. 151:589–598.PubMedCrossRefGoogle Scholar
  49. 49.
    Heck, L. W., Alarcon, P. G., Kulhavy, R. M., Morihara, K., Russel, M. W, and Mestecky, J. E., 1990, Degradation of IgA proteins by Pseudomonas aeruginosa elastase, J. Immunol. 144: 2253–2257.PubMedGoogle Scholar
  50. 50.
    Schultz, D. R., and Miller, K. D., 1974, Elastase of Pseudomonas aeruginosa: Inactivation of complement components and complement-derived chemotactic factors, Infect. Immun. 10: 128–135.PubMedGoogle Scholar
  51. 51.
    Scharmann, W, Jacob, E, and Porstendorfer, J., 1976, The cytotoxic action of leucocidin from Pseudomonas aeruginosa on human polymorphonuclear leukocytes, J. Gen. Microbial. 93:303–308.CrossRefGoogle Scholar
  52. 52.
    Kluftinger, J. L., Lutz, E, and Hancock, R. E. W, 1989, Pseudomonas aeruginosa cytotoxin: Periplasmic localization and inhibition of macrophages, Infect. Immun. 57:882–886.PubMedGoogle Scholar
  53. 53.
    Baltch, A. L., Hammer, M. C., Smith, R. P., Obrig, T. G., Conroy J. V., Bishop, M. B., Egy, M. A., and Lutz, E, 1985, Effects of Pseudomonas aeruginosa cytotoxin on human serum and granulocytes and their microbicidal, phagocytic and chemotactic functions, Infect. Immun. 48:498–506.PubMedGoogle Scholar
  54. 54.
    Lutz, E, Xiong, G., Jungblut, R., Orlik-Eisel, G., Gobel-Reifert, A., and Leidolf, R., 1991, Pore-forming cytotoxin of Pseudomonas aeruginosa: Molecular effects and aspects of pathogenicity, Antibiot. Chemother. 44:54–58.PubMedGoogle Scholar
  55. 55.
    Nonoyama, S., Kojo, H., Mine, Y., Nishida, M., Goto, S., and Kuwahara, S., 1979, Inhibitory effect of Pseudomonas aeruginosa on the phagocytic and killing activity of rabbit polymorphonuclear leukocytes: Mechanism of action of a polymorphonuclear leucokyte inhibitor, Infect. Immun. 24:399–403.PubMedGoogle Scholar
  56. 56.
    Laharrague, P. E., Corberand, J. X., Fillola, G., Gleizes, B. J., Fontanilles, A. M., and Gyrard, E., 1984, In vitro effect of the slime of Pseudomonas aeruginosa on the function of human polymorphonuclear neutrophils, Infect. Immun. 44:760–762.PubMedGoogle Scholar
  57. 57.
    Sensakovic, J. W., and Bartell, P. E., 1974, The slime of Pseudomonas aeruginosa: Biological characterization and possible role in experimental infection, J. Infect. Dis. 129: 101–109.PubMedCrossRefGoogle Scholar
  58. 58.
    Baltimore, R. S., and Mitchell, M., 1980, Immunologic investigations of mucoid strains of Pseudomonas aeruginosa: comparison of susceptibility to opsonic antibody in mucoid and non mucoid strains, J. Infect. Dis. 141:238–247.PubMedCrossRefGoogle Scholar
  59. 59.
    Schwarzmann, S., and Boring, J. R. III, 1971, Antiphagocytic effect of slime from a mucoid strain of Pseudomonas aeruginosa,Infect. Immun. 3:762–767.PubMedGoogle Scholar
  60. 60.
    Stire, H. G., Zachidniak, K., and Speert, D. P., 1988, Inhibition of polymorphonuclear chemotaxis by the mucoid exopolysaccharide of Pseudomonas aeruginosa,Clin.Invest.Med. 11: 247–252.Google Scholar
  61. 61.
    Pedersen, S. S., Høiby, N., Epersen, E., and Kharazmi, A., 1991, Alginate in infection, Antibiot. Chemother. 44:68–79.PubMedGoogle Scholar
  62. 62.
    Döring, G., Maier, M., Müller, E., Bibi, Z., Tümmler, B., and Kharazmi, A., 1987, Virulence factors of Pseudomonas aeruginosa, Antibiot. Chemother. 39:136–148.PubMedGoogle Scholar
  63. 63.
    Pollack, M., and Anderson, S. E., Jr., 1978, Toxicity of Pseudomonas aeruginosa exotoxin A for human macrophages, Infect.Immun. 19:1092–1096.PubMedGoogle Scholar
  64. 64.
    Stuart, R. K., Pollack, M., 1982, Pseudomonas aeruginosa exotoxin A inhibits proliferation of human bone marrow progenitor cells in vitro, Infect. Immun. 38:206–211.PubMedGoogle Scholar
  65. 65.
    Kharazmi, A., Høiby, N., Döring, G., and Valerius, N. H., 1984, Pseudomonas aeruginosa exoproteases inhibit human neutrophil chemiluminescence, Infect. Immun. 44:587–591.PubMedGoogle Scholar
  66. 66.
    Learn, D. B., Brestel, E. P., and Seetharama, S. 1987, Hypochlorite scavenging by Pseudomonas aeruginosa alginate, Infect. Immun. 55:1813–1818.PubMedGoogle Scholar
  67. 67.
    Garzelli, C., Colizzi, V., Campa, M., and Falcone, G., 1980, Mouse footpad infection by Pseudomonas aeruginosa: Evidence for delayed hypersensitivity to specific bacterial antigen, Med. Microbiol. Immunol. 168:111–118.PubMedCrossRefGoogle Scholar
  68. 68.
    Colizzi, V., Garzelli, C., Campa, M., Toca, L., and Falcone, G., 1982, Role of specific delayed-type hypersensitivity in Pseudomonas aeruginosa-infected mice, Immunology 47:337–344.PubMedGoogle Scholar
  69. 69.
    Campa, M., Toca, L., Lombardi, S., Garzelli, C., Colizzi, V, and Falcone, G., 1982, Cell-mediated immunity and delayed-type hypersensitivity in Pseudomonas aeruginosa-infected mice, Med. Microbiol. Immunol. 170:191–199.PubMedCrossRefGoogle Scholar
  70. 70.
    Markham, R. B., Pier, G. B., and Schreiber, J. R., 1991, The role of cytophilic IgG3 antibody in T cell-mediated resistance to infection with the extracellular bacterium, Pseudomonas aeruginosa, J. Immunol. 146:316–320.PubMedGoogle Scholar
  71. 71.
    Zehavi-Willner, T., 1988, Induction of murine cytolytic T lymphocytes by Pseudomonas aeruginosa exotoxin A, Infect. Immun. 56:213–218.PubMedGoogle Scholar
  72. 72.
    Parmely, M. J., Iglewski, B. H., and Horvat, R. T., 1984, Identification of the principal T Lymphocytes-stimulating antigens of Pseudomonas aeruginosa,J. Exp. Med. 160:1338–1349.PubMedCrossRefGoogle Scholar
  73. 73.
    Parmely, M. J., and Horvat, R. T., 1986, Antigenic specificity of Pseudomonas aeruginosa alkaline protease and elastase defined by human T cell clones, J. Immunol. 137:988–994.PubMedGoogle Scholar
  74. 74.
    Munster, A. M., and Leary, A. G., 1977, Cell-mediated immune responses to Pseudomonas aeruginosa,Am. J. Surg. 133:710–712.PubMedCrossRefGoogle Scholar
  75. 75.
    Porwoll, J. M., Gebel, H. M., Rodey, G. E., and Markham, R. B., 1983, In vitro response of human T cells to Pseudomonas aeruginosa, Infect. Immun. 40:670–674.PubMedGoogle Scholar
  76. 76.
    Munster, A. M., Winchurch, R. A., Burmingham, W. J., and Keeling, P., 1980, Longitudinal assay of lymphocyte responsiveness in patients with major burns, Ann. Surg. 192:772–775.PubMedCrossRefGoogle Scholar
  77. 77.
    Stone, H. H., Given, K. S., and Martin, J. D., 1967, Delayed rejection of skin homografts in Pseudomonas sepsis, Surg. Gynecol. Obstet. 124:1067–1070.PubMedGoogle Scholar
  78. 78.
    Floersheim, G. L., Hopff, W. H., Gasser, M., and Bucker, K., 1971, Impairment of cell-mediated immune responses by Pseudomonas aeruginosa, Clin. Exp. Immunol. 9:241–247.PubMedGoogle Scholar
  79. 79.
    Woodruff, M. F. A., Nolan, B., Robson, J. S., and MacDonald, M. K., 1969, Renal transplantation in man, Lancet i:6–12.CrossRefGoogle Scholar
  80. 80.
    Garzelli, C., Colizzi, V, Campa, M., Bozzi, L., and Falcone G., 1979, Depression of the antibody response in Pseudomonas aeruginosa-injected mice, Infect. Immun. 24:32–38.PubMedGoogle Scholar
  81. 81.
    Colizzi, V, Campa, M., Garzelli, C., Toca, L., Bevilacqua, G., and Falcone, G., 1982, Impairment of cell-mediated immunity in Pseudomonas aeruginosa pyelonephritis: Lack of supressor cell activity in vivo, Med. Microbiol. Immunol. 171:123–133.PubMedCrossRefGoogle Scholar
  82. 82.
    Campa, M., Garzelli, C., Ferrannini, E., and Falcone, G., 1976, Evidence for suppressor cell activity associated with depression of contact sensitivity in Pseudomonas aeruginosa infected mice, Clin. Exp. Immunol. 26:355–362.PubMedGoogle Scholar
  83. 83.
    Campa, M., Garzelli, C., and Falcone, G., 1975, Depression of contact sensitivity and enhancement of antibody response in Pseudomonas aeruginosa-infected mice, Infect. Immun. 12:1252–1257.PubMedGoogle Scholar
  84. 84.
    Colizzi, V., Garzelli, C., Campa, M., and Falcone, G., 1978, Depression of contact sensitivity by enhancement of suppressor cell activity in Pseudomonas aeruginosa-injected mice, Infect. Immun. 21:354–359.PubMedGoogle Scholar
  85. 85.
    Campa, M., Colizzi, V., Garzelli, C., Toca, L., and Falcone, G., 1980, Pseudomonas aeruginosa infection: activation of B suppressor cells which affect cell cooperation in the induction phase of contact sensitivity to oxazolone in mice, Curr. Microbial. 4:173–175.CrossRefGoogle Scholar
  86. 86.
    Garzelli, C., Colizzi, V., Campa, M., Bozzi, L., and Falcone, G., 1979, Depression of contact sensitivity by Pseudomonas aeruginosa-induced suppressor cells which affect the induction phase of immune response, Infect. Immun. 26:4–11.PubMedGoogle Scholar
  87. 87.
    Falcone, G., and Campa, M., 1981, Bacterial interference with the immune response, in: Microbial Perturbation of Host Defences (E O’Grady and H. Smith, eds.), Academic Press, London, pp. 185–210.Google Scholar
  88. 88.
    Benedettini, G., De Libero, G., Mori, L., and Campa, M., 1984, Staphylococcus aureus inhibits contact sensitivity to oxazolone by activating suppressor B cells in mice, Int. Arch. Allergy Appl. Immun. 73: 269–273.CrossRefGoogle Scholar
  89. 89.
    Campa, M., Benedettini, G., De Libero, G., Mori, L., and Falcone, G., 1984, T supressor cells as well as anti-hapten and anti-idiotype B lymphocytes regulate contact sensitivity to oxazolone in mice injected with purified protein derivative from Mycobacterium tuberculosis, Infect. Immun. 45:701–707.PubMedGoogle Scholar
  90. 90.
    Benedettini, G., De Libero, G., Mori, L., Marelli, E, Angioni, M. R., and Campa, M., 1985, Staphylococcus aureus-induced suppressor of contact sensitivity in mice. Suppressor cells elicited by polyclonal B-cell activation are regulated by idiotype anti-idiotype interactions, Cell. Immun. 93:508–519.CrossRefGoogle Scholar
  91. 91.
    Campa, M., Benedettini, G., and Marelli, P., 1986, B and T lymphocytes regulated by idiotype and anti-idiotype interactions inhibit delayed-type hypersensitivity to BCG in mice, Cell. Immun. 98:93–103.CrossRefGoogle Scholar
  92. 92.
    Campa, M., Marelli, P., Ota, E, Zolfino, I., Senesi, S., Malvaldi, G., 1989, B-cell-mediated depression of the granulomatous response to BCG in mice, Cell. Immun. 119:279–285.CrossRefGoogle Scholar
  93. 93.
    Powderly, W. G., Schreiber, J. R., Pier, G. B., and Markham, R. B., 1988, T cells recognizing polysaccharide-specific B cells function as contrasuppressor cells in the generation of T cell immunity to Pseudomonas aeruginosa, J. Immunol. 140:2746–2752.PubMedGoogle Scholar
  94. 94.
    Issekutz, T. B., and Stoltz, J. M., 1985, Suppression of lymphocyte proliferation by Pseudomonas aeruginosa: Mediation by Pseudomonas-activated suppressor monocytes, Infect. Immun. 48:832–838.PubMedGoogle Scholar
  95. 95.
    Petit, J.-C., Richard, G., Albert, B., and Daguet, G.-L., 1982, Depression by Pseudomonas aeruginosa of two T-cell-mediated responses, anti-Listeria immunity and delayed-type hypersensitivity to sheep erythrocytes, Infect. Immun. 35:900–908.PubMedGoogle Scholar
  96. 96.
    Holt, P. S., and Misfeldt, M. L., 1984, Alteration of murine immune response by Pseudomonas aeruginosa exotoxin A, Infect. Immun. 45:227–233.PubMedGoogle Scholar
  97. 97.
    Nutman, J., Berger, M., Chase, P. A., Dearborn, D. G., Miller K. M., Waller, R. L., and Sorensen, R. U., 1987, Studies on the mechanism of T cell inhibition by the Pseudomonas aeruginosa phenazine pigment pyocyanine, J. Immunol. 138:3481–3487.PubMedGoogle Scholar
  98. 98.
    Theander T. G., Kharazmi, A., Pedersen, B. K., Christensen, L. D., Tvede, N., Poulsen, L. K., Ødum, N., Svenson, M., and Bendtzen, K., 1988, Inhibition of human lymphocyte proliferation and cleavage of interluekin-2 by Pseudomonas aeruginosa proteases, Infect. Immun. 56:1673–1677.PubMedGoogle Scholar
  99. 99.
    Pedersen, B. K., Kharazmi, A., Theander, T. G., Odum, N., Andersen, V., and Bendtzen, 1987, Selective modulation of the CD4 molecular complex by Pseudomonas aeruginosa alkaline protease and elastase, Scand. J. Immunol. 26:91–94.PubMedCrossRefGoogle Scholar
  100. 100.
    Horvat, R. T., and Parmely, M. J., 1988, Pseudomonas aeruginosa alkaline protease degrades human gamma interferon and inhibits its bioactivity, Infect. Immun. 56:2925–2932.PubMedGoogle Scholar
  101. 101.
    Horvat, R. T., Clabaugh, M., Duval-Jobe, C., and Parmely, M. J., Inactivation of human gamma interferon by Pseudomonas aeruginosa proteases: Elastase augments the effects of alkaline protease despite the presence of a2-macroglobulin, Infect. Immun. 57:1668–1674.Google Scholar
  102. 102.
    Pedersen, B. K., and Kharazmi, A., 1987, Inhibition of human natural killer cell activity by Pseudomonas aeruginosa alkaline protease and elastase, Infect. Immun. 55:986–989.PubMedGoogle Scholar
  103. 103.
    Holt, P. S., and Misfeldt, M. L., 1985, Induction of an immune response in athymic nude mice to thymus dependent antigens by Pseudomonas aeruginosa exotoxin A, Cell. Immunol. 95:265–275.PubMedCrossRefGoogle Scholar
  104. 104.
    Garzelli, C., Campa, M., Colizzi, V., Benedettini, G., and Falcone, G., 1982, Evidence for autoantibody production associated with polyclonal B-cell activation by Pseudomonas aeruginosa, Infect. Immun. 35:13–19.PubMedGoogle Scholar
  105. 105.
    Garzelli, C., Campa, M., Forlani, A., Colizzi, V., and Falcone, G., 1982, LPS-induced enhancement of plaque-forming cell response to bromelain-treated syngeneic erythrocytes in mouse peritoneal cell cultures, Int. Arch. Allergy Appl. Immun. 67:143–147.CrossRefGoogle Scholar
  106. 106.
    Garzelli, C., Forlani, A., Lombardi, S., Colizzi, V., Campa, M., and Falcone, G., 1983, Regulation of the development of plaque-forming cells to bromelain-treated syngeneic mouse erytrocytes in bone marrow cell cultures, Int. Arch. Allergy Appl. Immun. 72:253–259.CrossRefGoogle Scholar
  107. 107.
    Campa, M., Garzelli, C., Colizzi, V., Benedettini, G., De Libero, G., and Falcone, G., 1983, Enhancement of the spontaneous development of autoreactive B cells by PPD in mouse peritoneal cell cultures, Microbiologica 6:199–205.PubMedGoogle Scholar
  108. 108.
    Chen, Y-H. U., Hancock, R. E. W, and Mishell, R. I., 1980, Mitogenic effects of purified outer membrane proteins from Pseudomonas aeruginosa, Infect. Immun. 28:178–184.PubMedGoogle Scholar
  109. 109.
    Papamichail, M., Dimitracopoulos, G., Tsokos, G., and Papavasiliou, J., 1980, A human lymphocyte mitogen extracted from the extracellular slime layer of Pseudomonas aeruginosa, J. Infect. Dis. 141:686–688.PubMedCrossRefGoogle Scholar
  110. 110.
    Pedersen, S. S., Høiby, N., Shand, G.H., and Pressler, T., 1989, Antibody response to Pseudomonas aeruginosa antigens in cystic fibrosis, Antibiot. Chemother. 42:130–153.PubMedGoogle Scholar
  111. 111.
    Döring, G., Obernesser, H. J., Boltzenhart, K., Flehming, B., Høiby, N., and Hofmann, A.., 1983, Proteases of Pseudomonas aeruginosa in cystic fibrosis, J. Infect. Dis. 147:744–750.PubMedCrossRefGoogle Scholar
  112. 112.
    Döring, G., Buhl, V., Høiby, N., Schiotz, P. O., and Botzenhart, K., 1984, Detection of proteases of Pseudomonas aeruginosa in immune complexes isolated from sputum of cystic fibrosis patients, Acta Pathol. Microbiol. Immunol. Scand. (C) 92:307–312.Google Scholar
  113. 113.
    Moss, R. B., and Hsu, Y-P., 1982, Isolation and characterization of circulating immune complexes in cystic fibrosis, Clin. Exp. Immunol. 47:301–308.PubMedGoogle Scholar
  114. 114.
    Campa, M., Ferrannini, E., Garzelli, C., Colizzi, V, and Falcone, G., 1979, Disturbance of lymphocyte circulation by Pseudomonas aeruginosa infection in oxazolone-sensitized mice, Curr. Microbiol. 2:283–286.CrossRefGoogle Scholar
  115. 115.
    Campa, M., Toca, L., Garzelli, C., Colizzi, V, Falcone, G., 1980, Reversal of Pseudomonas aeruginosa-induced disturbance of lymphocytes circulation in oxazolone-sensitized mice by cyclophosphamide, Immunol. Lett. 2:129–131.CrossRefGoogle Scholar
  116. 116.
    Falcone, G., and Campa, M., 1988, Diseases caused by Pseudomonas, in: Laboratory diagnosis of infectious diseases, Principles and Practice, (A. Balows, W J. Hausler, Jr., M. Ohashi, and A.. Turano, eds.), Springer-Verlag, New York, pp. 435–442.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Mario Campa
    • 1
  • Paola Marelli
    • 1
  • Antonella Lupetti
    • 1
  1. 1.Department of Biomedicine, Clinical Microbiology SectionUniversity of PisaPisaItaly

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