Abstract
Three different blood units were treated separately by the hypotonic dialysis (HD) and the dimethylsulphoxide osmotic pulse (DMSO) method, in order to load the erythrocytes with inositol hexaphosphate. A detailed comparison between the two loading techniques was performed by monitoring the red cell distribution patterns on discontinuous Percoll density gradients, the RBC oxygen affinity and the amount of the main intracellular organic phosphates with the 31P-NMR.
The results obtained showed that: (1) The HD loading produces a redistribution of the RBC fractions with a concomitant smoothing of the relative differences among distinct fractions (2) only a minor portion of erythrocytes (from 8.5 to 24.9% of total RBCs) are loaded with IHP after the DMSO treatment. All of these cells move to the lightest fraction (d = 1.080 g/ml). (3) Both HD and DMSO IHP-loaded cells show an increase in P50 (basal vs. after loading, means±SD: 25.8±3.0 vs. 52.5±3.2 mm Hg) correlated to the IHP incorporation (mean intracellular IHP concentration: 4.2 mmol/1 RBC). (4) probably the IHP incorporation efficiency could be probably improved at least by increasing the IHP concentration during the treatment.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
B. Teisseire, C. Ropars, M.O. Valiez, R.A. Herigault and C. Nicolau, Physiological effects of high-P50 erythrocyte transfusion on piglets, J. Appl. Physiol. 58: 1810 (1985).
J.F. Baron, E. Vicault, O. Stucker, M.C. Villereal, C. Ropars, B. Teisserie and M. Duvelleroy, Isolated heart as a model to study the effects of the decrease in oxygen hemoglobin affinity, Advances in the Biosciences 67: 73 (1987).
A. Zanella, L. Sabbioneda, F. Rossi, M.B. Colombo, V. Russo, C. Aguggini, G. Fiorelli, S. Villa, A. Brovelli, M.A. Castellana, C. Seppi, C. Barlassina and Sirchia G., IHP- loaded red cells: in vitro and in vivo properties, Advances in the Biosciences 81: 219 (1991).
M. Samaja, R. Motterlini, N. Tarantola, M. Beretta, F. Rossi, L. Sabbioneda, M. Porcellati and A. Zanella, Viability of the IHP-loaded red cell in the hypoperfused isolated rat heart, Advances in the Biosciences 81: 181 (1991).
R.S. Franco, K. Wagner, M. Weiner and O.J. Martelo, Incorporation of inositol hexaphosphate into red blood cells mediated by dimethyl sulphoxide, Life Sci. 32: 2763 (1983).
A. Mosca, R. Paleari, A. Modenese, S. Rossini, R. Parma, C. Rocco, V. Russo, G.C. Caramenti, M.L. Paderi, A. Sanna and R. Galanello, Clinical utility of fractionating erythrocytes into “Percoll” density gradients, in: Red Cell Aging M. Magnani and A. De Flora, eds. Plenum Publishing Co, New York (1992).
A. Hayashi, T. Suzuki and H. Shin, An enzymic reduction system for metmyoglobin and methemoglobin and its application to functional studies of oxygen carriers, Biochem. Biophys. Acta 310: 309 (1973).
R. Nano, A. Mosca, R. Paleari and A. Boicelli, Quantitative IHP determination by 31P-NMR: proposal for a standardized protocol, This Meeting.
C. Nicolau, B.P. Teisseire, C. Ropars, M.O. Vallez and R.A. Herigault, Incorporation of allosteric effetors of hemoglobin in red blood cells. Physiological effects, in: “Red Blood Cells as Carriers for Drugs” J.R. DeLoach and U. Sprandel, eds., Karger, Basel (1985).
R.S. Franco and R.L. Barker, Modification of the oxygen affinity and intracellular hemoglobin concentration of normal and sickle cells by means of an osmotic pulse, J. Lab. Clin. Med. 113: 58 (1989).
M. Langer, P. Prato, S. Villa, F. Rossi, P. Di Mauro, S. Gatti, A. Piazzini, B. Gridelli, G. Fiorelli and A. Zanella, A baboon model to test physiological and adverse effects of human red cells loaded with inositol hexaphosphate (IHP). This Meeting.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 Springer Science+Business Media New York
About this chapter
Cite this chapter
Mosca, A. et al. (1992). IHP Entrapment into Human Erythrocytes: Comparison between Hypotonic Dialysis and DMSO Osmotic Pulse. In: Magnani, M., DeLoach, J.R. (eds) The Use of Resealed Erythrocytes as Carriers and Bioreactors. Advances in Experimental Medicine and Biology, vol 326. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3030-5_2
Download citation
DOI: https://doi.org/10.1007/978-1-4615-3030-5_2
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6321-7
Online ISBN: 978-1-4615-3030-5
eBook Packages: Springer Book Archive