Abstract
Methods are reported for the quantitation and isolation of biotinylated red blood cells (B- RBC). The first method is for determination of posttransfusional survival of rabbit RBC by flow cytometry. The survival of B-RBC was measured using both fresh and paraformaldehyde-fixed cells with similar results. The posttransfusion survival of rabbit RBC measured in this way was normal. There was no indication of increased cell destruction due to antibodies directed against B-RBC and no evidence for loss of biotin from circulating cells. The second methodology is for the isolation of B-RBC from blood with streptavidin-coated magnetic beads. At least eighty percent of positive cells were recovered with very few false positives. Both methods may be helpful in the study of resealed erythrocytes.
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© 1992 Springer Science+Business Media New York
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Russo, V., Barker-Gear, R., Gates, R., Franco, R. (1992). Studies with Biotinylated RBC: (1) Use of Flow Cytometry to Determine Posttransfusion Survival and (2) Isolation Using Streptavidin Conjugated Magnetic Beads. In: Magnani, M., DeLoach, J.R. (eds) The Use of Resealed Erythrocytes as Carriers and Bioreactors. Advances in Experimental Medicine and Biology, vol 326. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3030-5_12
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DOI: https://doi.org/10.1007/978-1-4615-3030-5_12
Publisher Name: Springer, Boston, MA
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