Further Characterization of Aminopeptidase-N as a Receptor for Coronaviruses
Part of the
Advances in Experimental Medicine and Biology
book series (AEMB, volume 342)
We recently reported that porcine aminopeptidase-N (pAPN) acts as a receptor for transmissible gastroenteritis virus (TGEV). In the present work, we addressed the question of whether TGEV tropism is determined only by the virus-receptor interaction. To this end, different non-permissive cell lines were transfected with the porcine APN cDNA and tested for their susceptibility to TGEV infection. The four transfected cell lines shown to express pAPN at their membrane became sensitive to infection. Two of these cell lines were found to be defective for the production of viral particles. This suggests that other factor(s) than pAPN expression may be involved in the production of infectious virions. The pAPN-transfected cells were also tested for their susceptibility to several viruses which have a close antigenic relationship to TGEV. So far, we failed to evidence permissivity to the feline infectious peritonitis coronavirus FIPV and canine coronavirus CCV. In contrast, we found clear evidence that porcine respiratory coronavirus PRCV, a variant of TGEV which replicates efficiently in the respiratory tract but to a very low extent in the gut, may also utilise APN to gain entry into the host cells. This suggests that the switch between TGEV and PRCV tropisms in vivo may involve other determinant(s) than receptor recognition.
KeywordsTitration Sedimentation Peritonitis Hydrolase Neomycin
S. Siddell, H. Wege and V. Ter Meulen, The biology of coronaviruses, J. gen. Virol.
64:761 (1983).PubMedCrossRefGoogle Scholar
M. Pensaert, E.O. Haelterman and T. Burstein, Transmissible gastroenteritis of swine: virus-intestinal cell-interactions, Arch. Gesamte. Virusforch.
31:321 (1970).CrossRefGoogle Scholar
K. McIntosh, in Virology
2nd edn (eds Fields, D.N. et al.)
pp857, Raven, New York (1990).Google Scholar
B. Delmas, J. Gelfi, R. L’Haridon, L. K. Vogel, H. Sjöström, O. Noren and H. Laude, Aminopeptidase N is a major receptor for the enteropathogenic coronavirus TGEV, Nature
357:417 (1992).PubMedCrossRefGoogle Scholar
C.T. Yeager, R.A. Ashmun, R.K. Williams, C.B. Cardellichio, L.H. Shapiro, A.T. Look and K.V. Holmes, Human aminopeptidase N is a receptor for human coronavirus 229E, Nature
357:420 (1992).PubMedCrossRefGoogle Scholar
A.J. Kenny and S. Maroux, Topology of microvillar membrane hydrolases of kidney and intestine, Physiol. Rev.
62:91 (1982).PubMedGoogle Scholar
A.T. Look, R.A. Ashmun, L.H. Shapiro and S.C. Peiper, Human myeloid plasma membrane glycoprotein CD13 (gp150) is identical to aminopeptidase N, J. clin. Invest.
83:1299 (1989).PubMedCrossRefGoogle Scholar
E.O. Haelterman, On the pathogenesis of transmissible gastroenteritis of swine, J. Am. vet. med. Assoc.
160:534 (1972).Google Scholar
R.D. Woods, N.F. Cheville and J.E. Gallagher, Lesions in the small intestine of newborn pigs inoculated with porcine, feline and canine coronaviruses, Am. J. vet. Res.
42:1163 (1981).PubMedGoogle Scholar
H. Laude, K. Van Reeth and M. Pensaert, Porcine respiratory coronavirus: molecular features and virus-host interactions, Vet. Res.
24:125 (1993).PubMedGoogle Scholar
W. Kueng, E. Silber and U. Eppenberger, Quantification of cells cultured on 96-well plates, Analyt. Biochem.
182:16 (1989).PubMedCrossRefGoogle Scholar
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