Abstract
A papainlike cysteine protease (PCP) domain in the N-terminal region of the equine arteritis virus (EAV) replicase was identified by in vitro translation and mutagenesis studies. The EAV protease was found to direct an autoproteolytic cleavage at its C-terminus which leads to the production of an approximately 30K N-terminal replicase product (nspl) containing the PCP domain. Amino acid residues Cys164 and His230 of the EAV replicase polyprotein were identified as the most likely candidates for the role of PCP catalytic residues. It was sshown that cleavage occurs in cis between Gly260 and Gly261
Chapter PDF
Similar content being viewed by others
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
P.G.W. Plagemann and V. Moennig, Adv. Virus Res. 41:99.(1991).
J.A. den Boon, E.J. Snijder, E.D. Chirnside, A.A.F. de Vries, M.C. Horzinek and W.J.M. Spaan, J. Virol. 65:2910 (1991).
A.A.F. de Vries, E.D. Chirnside, P.J. Bredenbeek, L.A. Gravestein, M.C. Horzinek and W.J.M. Spaan, Nucleic Acids Res. 18:3241 (1990).
W.J.M. Spaan, D. Cavanagh and M.C. Horzinek, J. Gen. Viral. 69:2939 (1988).
E.J. Snijder, M.C. Horzinek and W.J.M. Spaan, J. Virol. 64:331 (1990).
E.J. Snijder, J.A. den Boon, P.J. Bredenbeek, M.C. Horzinek, R. Rijnbrand and W.J.M. Spaan,Nucleic Acids Res. 18:4535 (1990).
M.E.G. Boursnell, T.D.K. Brown, I.J. Foulds, P.F. Green, F.M. Tomley and M.M. Binns, J. Gen. Virol. 68:57 (1987).
P.J. Bredenbeek, C.J. Pachuk, J.F.H. Noten, J. Charité, W. Luytjes, S.R. Weiss and W.J.M. Spun, Nucleic Acids Res. 18:1825 (1990).
H.J. Lee, C.K. Shieh, A.E. Gorbalenya, E.V. Koonin, N. la Monica, J. Tuler, A. Bagdzhadzhyan and M.M.C. Lai, Virology 180:567 (1991).
I. Brierley, P. Diggard and S.C. Inglis, Cell 57:537 (1989).
L. Polgar and P. Halasz, Biochem. J. 207:1 (1982).
M.R. Denison, P.W. Zoltick, J.L. Leibowitz, C.J. Pachuk and S.R. Weiss, J. Virol. 65:3067 (1991).
A.E. Gorbalenya, E.V. Koonin, A.P. Donchenko and V.M. Blinov, Nucleic Acids Res. 17:4847 (1989).
M.R. Denison, P.W. Zoltick, S.A. Hughes, B. Giangreco, A L. Olson, S. Perlman, J.L. Leibowitz and S.R. Weiss, Virology 189:274 (1992).
A.E. Gorbalenya, E.V. Koonin and M.M.C. Lai, FEBS Lett. 288:201 (1991).
J.H. Strauss and E.G. Strauss, Semin. Virol. 1:347 (1991).
J.C. Carrington and K.L. Herndon, Virology 187:308 (1992).
C.-S. Oh and J.C. Carrington, Virology 173: 692 (1989).
G.H. Choi, D.M. Pawlyk and D.L. Nuss, Virology 183:747 (1991).
E.V. Koonin, G.H. Choi, D.L. Nuss, R. Shapira and J.C. Carrington, Proc. Natl. Acad. Sci. USA 88:10647 (1991).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1994 Springer Science+Business Media New York
About this chapter
Cite this chapter
Snijder, E.J., Wassenaar, A.L.M., Spaan, W.J.M. (1994). Proteolytic Processing of the N-terminal Region of the Equine Arteritis Virus Replicase. In: Laude, H., Vautherot, JF. (eds) Coronaviruses. Advances in Experimental Medicine and Biology, vol 342. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2996-5_36
Download citation
DOI: https://doi.org/10.1007/978-1-4615-2996-5_36
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6305-7
Online ISBN: 978-1-4615-2996-5
eBook Packages: Springer Book Archive