Formation, Inhibition of Formation, and Repair of Oxidative 8-Hydroxyguanine DNA Damage

  • H. Kasai
  • M.-H. Chung
  • F. Yamamoto
  • E. Ohtsuka
  • J. Laval
  • A. P. Grollman
  • S. Nishimura
Part of the Basic Life Sciences book series (BLSC, volume 61)


Oxygen radicals are produced by many carcinogenic agents and by ionizing radiation, and cause DNA damage in vitro and in vivo. Oxygen radicals are also produced in vivo during cellular metabolism of oxygen. Therefore, studies on DNA damage induced by oxygen radicals should be helpful in elucidating the mechanisms of induction of cancer by ionizing radiation and environmental chemicals as well as spontaneous carcinogenesis during aging.


High Performance Liquid Chromatography Oxygen Radical Nickel Compound Cigarette Smoke Condensate Potassium Bromate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Cheng, K.C., D.S. Cahill, H. Kasai, S. Nishimura, and L.A. Loeb (1991) The oxygen free radical associated DNA adduct 8-oxoguanine causes G•C to T•A transversions during in vivo DNA replication. Proceedings, Am. Assoc. Cancer Res. 32:149.Google Scholar
  2. 2.
    Chung, M.H., H. Kasai, D.S. Jones, H. Inuoe, H. Ishikawa, E. Ohtsuka, and S. Nishimura (1990) An endonuclease activity of Escherichia coli that specifically removes 8-hydroxyguanine residues from DNA. Mutat. Res. 254:1–12.CrossRefGoogle Scholar
  3. 3.
    Djuric, Z., and D.W. Potter (1990) Oxidative DNA damage by 1,6 dinitropyrene in vivo. Proceedings, Am. Assoc. Cancer Res. 31:146.Google Scholar
  4. 4.
    Fiala, E.S., C.C. Conaway, and J.E. Mathis (1989) Oxidative DNA and RNA damage in the liver of Sprague-Dawley rats treated with the hepatocarcinogen 2-nitropropane. Cancer Res. 49:5518–5522.Google Scholar
  5. 5.
    Floyd, R.A., J.J. Watson, P.K. Wong, D.H. Altmiller, and R.C. Rickard (1986) Hydroxyl free radical adduct of deoxyguanosine: Sensitive detection and mechanisms of formation. Free Radicals Res. Comm. 1:163–172.CrossRefGoogle Scholar
  6. 6.
    Hinrichsen, L.I., R.A. Floyd, and O. Sudilovsky (1990) Is 8 hydroxydeoxyguanosine a mediator of carcinogenesis by a choline-devoid diet in the rat liver? Carcinogenesis 11:1879–1881.PubMedCrossRefGoogle Scholar
  7. 7.
    Kasai, H., S. Nishimura (1983) Hydroxylation of the C-8 position of deoxyguanosine by reducing agents in the presence of oxygen. Nucl. Acids Res. Symp. Ser. 12:165–167.Google Scholar
  8. 8.
    Kasai, H., and S. Nishimura (1984) Hyroxylation of deoxyguanosine at the C-8 position by ascorbic acid and other reducing agents. Nucl. Acids Res. 12:2137–2145.CrossRefGoogle Scholar
  9. 9.
    Kasai, H., and S. Nishimura (1988) Formation of 8-hydroxdeooxyguanosine in DNA by auto-oxidized unsaturated fatty acids. In Medical, Biochemical and Chemical Aspects of Free Radicals ,O. Hayaishi, E. Niki, M. Kondo, and T. Yoshikawa, eds. Elsevier Science Publishers, pp. 1021–1023.Google Scholar
  10. 10.
    Kasai, H., and S. Nishimura (1991) Formation of 8-hydroxydeoxyguanosine in DNA by oxygen radicals and its biological significance. In Oxidative Stress, Oxidants and Antioxidants ,H. Sies, ed. Academic Press Ltd., pp. 99–116.Google Scholar
  11. 11.
    Kasai, H., P.F. Crain, Y. Kuchino, S. Nishmura, A. Ootsuyama, and H. Tanooka (1986) Formation of 8-hydroxyguanine moiety in cellular DAN by agents producing oxygen radicals and evidence for its repair. Carcinogenesis 7:1849–1851.PubMedCrossRefGoogle Scholar
  12. 12.
    Kasai, H., S. Nishimura, Y. Kurokawa, and Y. Hayashi (1987) Oral administration of the renal carcinogen, potassium bromate, specifically produces 8-hydroxydeoxyguanosine in rat target organ DNA. Carcinogenesis 8:1959–1961.PubMedCrossRefGoogle Scholar
  13. 13.
    Kasai, H., Y. Okada, S. Nishimura, M.S. Rao, and J.K. Reddy (1989) Formation of 8-hydrooxydeoxyguanosine in liver DNA of rats following longterm exposure to a peroxisome proliferator. Cancer Res. 49:2603–2605.Google Scholar
  14. 14.
    Kasprzak, K.S., and L. Hernandez (1989) Enhancement of hydroxylation and deglycosylation of 2’-deoxyguanosine by carcinogenic nickel compounds. Cancer Res. 49:5964–5968.Google Scholar
  15. 15.
    Kasprzak, K.S., K. Higinbotham, B.A. Diwan, A.O. Perantoni, and J.M. Rice (1990) Correlation of DNA base oxidation with the activation of K~ras oncogene in nickel-induced renal tumors. Free Rad. Res. Comm. 9(Suppl. 1):l72.Google Scholar
  16. 16.
    Kuchino, Y., F. Mori, H. Kasai, H. Inoue, S. Isai, K. Miura, E. Ohtsuka, and S. Nishimura (1987) Misreading of DNA templates containing 8 hydroxydeoxyguanosine at the modified base and at adjacent residues. Nature 327:77–79.PubMedCrossRefGoogle Scholar
  17. 17.
    Moriya, M., C.O.V. Bodpudi, F. Johnson, M. Takeshita, and A.P. Grollman (1991) Site specific mutagenesis using a gapped duplex vector: A study of translesion synthesis past 8-oxodeoxyguanosine in E. coli. Mutat. Res. 254:281–288.CrossRefGoogle Scholar
  18. 18.
    Nair, U.J., R.A. Floyd, J. Nair, V. Bussachini, M. Friesen, and H. Bartsch (1987) Formation of reactive oxygen species and of 8-hydroxyguanosine in DNA in vitro with betel quid ingredients. Chem. Biol. Interact. 63:157–169.CrossRefGoogle Scholar
  19. 19.
    Nakae, D., H. Yoshiji, H. Maruyama, T. Kinugasa, A. Denda, and Y. Knoishi (1990) Production of both 8-hydroxydeoyguanosine in liver DAN and g glutamyltransferase-positive hepatocellular lesion in rats given a cholinedeficient, L-amino acid-defined diet. Japan. J. Cancer Res. 81:1081–1084.CrossRefGoogle Scholar
  20. 20.
    Shibutani, S., M. Takeshita, and A.P. Grollman (1991) Insertion of specific bases during DNA synthesis past the oxidation-damaged base 8-oxodG. Nature 349:431–434.PubMedCrossRefGoogle Scholar
  21. 21.
    Tchou, J., H. Kasai, S. Shibutani, M.H. Chung, J. Laval, A.P. Grollman, and S. Nishimura (1991) 8-Oxoguanine (8-hydroxyguanine) DNA glycosylase and its substrate specificity. 88:4690–4694.Google Scholar
  22. 22.
    Umemura, T., K. Sai, A. Takagi, R. Hasegawa and Y. Kurokawa (1990) Formation of 8-hydroxydeoxyguanosine (8-OH-dG) in rat kidney DNA after intraperitoneal administration of ferric nitrilotriacetate (Fe-NTA). Carcinogenesis 11:345–347.PubMedCrossRefGoogle Scholar
  23. 23.
    Wood, M.L., M. Dizdaroglu, E. Gajewski, and J.M. Essigmann (1990) Mechanistic studies of ionizing radiation and oxidative mutagenesis: Genetic effects of a single 8-hydroxyguanine (7-hydro-8-oxoguanine) residue inserted at a unique site in a viral genome. Biochemistry 29:7024–7032.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • H. Kasai
    • 1
  • M.-H. Chung
    • 2
  • F. Yamamoto
    • 1
  • E. Ohtsuka
    • 3
  • J. Laval
    • 4
  • A. P. Grollman
    • 5
  • S. Nishimura
    • 1
  1. 1.Biology DivisionNational Cancer Center Research InstituteTokyoJapan
  2. 2.Department of Pharmacy, College of MedicineSeoul National UniversitySeoulKorea
  3. 3.Department of Bioorganic Chemistry, Faculty of Pharmaceutical SciencesHokkaido UniversitySapporoJapan
  4. 4.Institut Gustave-RoussyVillejuifFrance
  5. 5.Department of Pharmaceutical SciencesState University of New YorkStony BrookUSA

Personalised recommendations