Dopamine Metabolism in the Rabbit Carotid Body in Vitro: Effect of Hypoxia and Hypercapnia

  • L-M Leitner
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 337)

Summary

Dopamine (DA) and noradrenaline (NA) were measured in the rabbit carotid body (CB) in vitro by HPLC-ED under the following experimental conditions: 1 h superfusion in normoxic, hypoxic (10% O2 in N2) or hypercapnic (8% CO2, 20% O2, 72% N2) medium, 5h superfusion in normoxia or hypoxia. The contents of DA and NA were decreased by hypoxia and hypercapnia after 1 h and 5h indicating a possible DA and NA secretion. Under the same experimental conditions synthesis of DA and NA and catabolism of DA were studied with enzymatic inhibition of tyrosine hydroxylase and monoamine oxidase (MAO) respectively. In hypoxia (1 h and 5h) the rate constant of DA synthesis was the same as in normoxia; however NA synthesis was decreased after 1 h hypoxia. On the contrary, hypercapnia, appeared to be a very effective stimulus of DA and NA synthesis.

Keywords

Sucrose Dopamine Tyrosine MgCl CaCl 

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References

  1. Brodie B B.,Costa E.,Dlabac A.,Neff N. N.,Smookler H.H.,1966, Application of steady state kinetics to the estimation of synthesis rate and turnover time of tissue catecholamines. J. Pharmacol Exp. Ther.,154: 493.PubMedGoogle Scholar
  2. Brokaw J J., Hansen J. T., Christie D. S., 1985, The effects of hypoxia on catecholomine dynamics in the rat carotid body. J. Auton. Nerv. Syst., 13: 35.PubMedCrossRefGoogle Scholar
  3. Brown R.M., Snider S.R., Carlsson A., 1974, Changes in biogenic amine synthesis and turnover induced by hypoxia and/or foot shock stress. 11. The central nervous system. J. Neural Trans., 35:293.CrossRefGoogle Scholar
  4. Carlsson A., Holmin T., Lindqvist M J.,Siesjö B.K., 1977, Effect of hypercapnia and hypercarbia on tryptophan and tyrosine hydroxylation in rat brain. Acta Physiol. Scand., 99: 503.PubMedCrossRefGoogle Scholar
  5. Dalmaz Y., Pequignot J.-M., Tavitian E., Cottet- Emard.M., Peyrin L., 1988, Long-term hypoxia increase the turnover of dopamine but not norepinephrine in rat sympathetic ganglia. J. Auton. Nerv. Syst., 24: 57.PubMedCrossRefGoogle Scholar
  6. Davis J.N., Carlsson A., 1973, The effect of hypoxia on monoamine synthesis, levels and metabolism in rat brain. J. Neurochem., 21 783.PubMedCrossRefGoogle Scholar
  7. Feinsilver S.H., Wong R., Raybin D.M., 1987, Adaptations of neurotransmitter synthesis to chronic hypoxia in cell culture Biochim. Biophys. Acta, 928: 56.CrossRefGoogle Scholar
  8. Fidone S., Gonzalez C., 1982a, Catecholamines synthesis in rabbit carotid body in vitro. J. Physiol. (London), 333: 69.Google Scholar
  9. Fidone S.,Gonzalez C.,Yoshizaki K., 1982b, Effects of hypoxia on catecholamine synthesis in rabbit carotid body in vitro. J. Physiol. (London), 333 81.Google Scholar
  10. Fidone S.J., Gonzalez C., 1986, Initiation and control of chemoreceptor activity in the carotid body;In, Handbook of Physiology, Section 3: The Respiratory system, Vol. 11. Control of Breathing, Part I, pp 247, AP. Fishman ed. Am. Physiol. Soc. Bethesda. Md.Google Scholar
  11. Freeman GB., Gibson G.E., 1986, Effect of decreased oxygen on in vitro release of endogenous 3,4dihydroxyphenylethylamine from mouse striatum. J. Neurochem., 47: 1924.PubMedCrossRefGoogle Scholar
  12. Hanbauer I., Hellstrom S., 1978, The regulation of dopamine and noradrenaline in the rat carotid body and its modification by denervation and by hypoxia. J. Physiol. (London), 282: 21.Google Scholar
  13. Hellstrom S.,Pequignot J.M.,Dahlqvist A.,1989, Catecholamines in the carotid body are unaffected by hypercapnia. Neurosci, Lett., 97: 280.CrossRefGoogle Scholar
  14. Kuno T.,Marukawa A.,Fujiwara H.,Tanaka C.,1981. Dopamine accumulation in the mouse brain during hypoxia. Japan. J. Pharmacol.. 31: 503.Google Scholar
  15. Leitner L.-M., Roumy M., 1986, Chemoreceptor response to hypoxia and hypercapnia in catecholamine depleted rabbit and cat carotid bodies in vitro. Pfliigers Arch., 406: 419.CrossRefGoogle Scholar
  16. Leitner L.-M., Roumy M., Ruckebusch M., Sutra J.F., 1986, Monoamines and their catabolites in the rabbit carotid body. Effects of reserpine, sympathectomy and carotid sinus nerve section. Pfltigers Arch., 406: 552.CrossRefGoogle Scholar
  17. Miwa S., Fujiwara M., Inoue M., Fujiwara M., 1986, Effects of hypoxia on the activities of noradrenergic and dopaminergic neurons in the rat brain. J. Neurochem., 47: 63.Google Scholar
  18. Nagatsu T., Levitt M., Udenfriend S.,1964, Tyrosine hydroxylase. The initial step in norepinephrine biosynthesis. J. Biol, Chem.. 239: 2910.PubMedCrossRefGoogle Scholar
  19. Rigual R., Gonzalez E., Fidone S., Gonzalez C., 1984, Effects of low pH on synthesis and release of catecholamines in the cat carotid body. Brain Res., 309:178.PubMedCrossRefGoogle Scholar
  20. Rigual R., Gonzalez E., Gonzalez C., Fidone S., 1986, Synthesis and release of catecholamines by the cat carotid body in vitro. Brain Res., 374: 101.PubMedCrossRefGoogle Scholar
  21. Roumy M.,Armengaud C.,Ruckebusch M.,Sutra J.F.,Leitner L.-M.,1988, Fate of the catecholamine stores in the rabbit carotid body superfused in vitro. Pfliigers Arch., 411–436.Google Scholar
  22. Roumy M.,Ruckebusch M.,Sutra J.F., Leitner L.-M., 1986, Rate of dopamine metabolism in the rabbit carotid body in vivo. Pfliigers Arch., 407: 575.CrossRefGoogle Scholar
  23. Stone E A.,1983, Rapid adaptation of the stimulatory effect of CO2 on brain norepinephrine metabolism.Naunyn-Schmiedeberg’s Arch. Pharmacol., 324: 313.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • L-M Leitner
    • 1
    • 2
  1. 1.Univ. P.-Sabatier, Fac. Méd., Lab. Physiol., UA CNRS 649, 133 Rte de NarbonneToulouseFrance
  2. 2.Lab. Physiol. Pharmacol. 23ToulouseFrance

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