Advertisement

Cascade Control of Ammonia Assimilation

  • Wally C. van Heeswijk
  • Hans V. Westerhoff
  • Daniel Kahn

Abstract

In cellular physiology, metabolism is organized at several levels: the level of intermediary metabolism, the level of protein metabolism (synthesis, modification and degradation), and the level of mRNA metabolism (transcription and decay). At the level of protein metabolism we study cascades of enzymes modifying one another covalently. To this purpose metabolic control theory (MCT) has been extended explicitly to include regulatory cascades. The resulting modular control theory1, which started from the general formalism of Reder2, will be used to analyze the glutamine synthetase regulatory cascade in Escherichia coli. The modular control analysis of this cascade should give insight into how control is distributed over the different hierarchical levels of the cascade, and to quantitate the relative importance of metabolic and genetic regulation of nitrogen assimilation.

Keywords

Glutamine Synthetase Glutamate Dehydrogenase Nitrogen Assimilation RNAse Protection Assay Ammonia Assimilation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    D. Kahn and H.V. Westerhoff, Control theory of regulatory cascades, J. theor. Biol. 153:255–285 (1991).PubMedCrossRefGoogle Scholar
  2. 2.
    C. Reder, Metabolic control theory: a structural approach, J. theor. Biol. 135:175–201 (1988).PubMedCrossRefGoogle Scholar
  3. 3.
    S.G. Rhee, W.G. Bang, J.H. Koo, K.H. Min and S.C. Park, 1988, Regulation of the glutamine synthetase activity and its biosynthesis in Escherichia coli: mediation by three cycles of covalent modification, in: “Enzyme dynamics and regulation,” P. Boon-Chock, C.Y. Huang, C. L. Tsou, J.H. Wang, eds, Springer-Verlag, Berlin, pp. 136–145 (1988).CrossRefGoogle Scholar
  4. 4.
    W. van Heeswijk, M. Rabenberg, H.V. Westerhoff and D. Kahn, The genes of the glutamine synthetase adenylylation cascade are not regulated by nitrogen in Escherichia coli, Mol. Microbiol. ,in press.Google Scholar
  5. 5.
    H.S. Son and S.G. Rhee, 1987, Cascade control of Escherichia coli glutamine synthetase. Purification and properties of PII protein and nucleotide sequence of its structural gene, J. Biol. Chem. 262:8690– 8695 (1987).PubMedGoogle Scholar
  6. 6.
    P.R. Jensen, H.V. Westerhoff and O. Michelsen, On the use of loc-type promoters in control analysis, Eur. J. Biochem. ,in press.Google Scholar
  7. 7.
    J. Collado-Vides, B. Magasanik and J.D. Gralla, Control site location and transcriptional regulation in Escherichia coli, Microbiol. Rev. 55:371–394 (1991).PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Wally C. van Heeswijk
    • 1
    • 2
  • Hans V. Westerhoff
    • 1
    • 2
  • Daniel Kahn
    • 2
  1. 1.E.C. Slater InstituteUniversity of AmsterdamAmsterdamThe Netherlands
  2. 2.Division of Molecular BiologyThe Netherlands Cancer Institute (H5)AmsterdamThe Netherlands

Personalised recommendations