The Redox State of Cytochromes as a New Tool to Assess the Thermodynamic State of Mitochondria
In the present work we have followed the changes of the absorption spectra of mitochondrial suspensions during transitions from anaerobiosis to steady state and after addition of uncouplers. From these spectra we have selected proper wavelengths to follow the kinetics of the spectroscopic changes of the various cytochrome components of the redox chain. We focused our attention to a group of cytochromes, those of the cc1, providing a response similar in significance to that of the electrodes or of the dyes assessing the membrane potential.
KeywordsSucrose Depression Titration Respiration Catalase
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